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RUNX2 elisa kit :: Human Runt-related transcription factor 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS766006
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
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 Product Name   

Runt-related transcription factor 2 (RUNX2), ELISA Kit

★Popular Item★
 Also Known As   

Human Runt-related transcription factor 2 ELISA Kit

 Product Synonym Names    RUNX2/CBFA1/Acute myeloid leukemia 3 protein/AML3CCD/Core-binding factor subunit alpha-1/PEA2-alpha A/PEBP2A/PEBP2A1CCD1/PEBP2aA1/PEBP2-alpha A/runt domain; alpha subunit 1/runt-related transcription factor 2/SL3,AKV core-binding factor alpha A subunit/SL3-3 enhancer factor 1 alpha A subunit
 Product Gene Name   

RUNX2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS766006 QC Report    QC Report PDF
 OMIM    119600
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of RUNX2. No significant cross-reactivity or interference between RUNX2 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.313-20ng/ml
 Sensitivity    0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RUNX2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RUNX2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RUNX2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RUNX2 purchase    MBS766006 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Runt-related transcription factor 2 (RUNX2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/ml in biological research samples containing RUNX2, with an estimated sensitivity of 0.188ng/ml. The ELISA analytical biochemical technique of the MBS766006 kit is based on RUNX2 antibody-RUNX2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RUNX2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RUNX2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RUNX2 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- RUNX2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- RUNX2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RUNX2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RUNX2 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of RUNX2 elisa kit    RUNX2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS766006. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RUNX2. It may not necessarily be applicable to this product.
 NCBI GI #    226442783
 NCBI GeneID    860
 NCBI Accession #    NP_001019801.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001024630.3 [Other Products]
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 UniProt Secondary Accession #    O14614; O14615; O95181 [Other Products]
 UniProt Related Accession #    Q13950 [Other Products]
 Molecular Weight    54,249 Da
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 NCBI Official Full Name    runt-related transcription factor 2 isoform a
 NCBI Official Synonym Full Names    runt related transcription factor 2
 NCBI Official Symbol    RUNX2 [Similar Products]
 NCBI Official Synonym Symbols   
CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
[Similar Products]
 NCBI Protein Information    runt-related transcription factor 2
 UniProt Protein Name    Runt-related transcription factor 2
 UniProt Synonym Protein Names   
Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
 Protein Family    Runt-related transcription factor
 UniProt Gene Name    RUNX2 [Similar Products]
 UniProt Synonym Gene Names    AML3; CBFA1; OSF2; PEBP2A; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A [Similar Products]
 UniProt Entry Name    RUNX2_HUMAN
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 NCBI Summary for RUNX2    This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008]
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 UniProt Comments for RUNX2    AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; transcription factor complex

Molecular Function: ATP binding; bHLH transcription factor binding; chromatin binding; histone deacetylase binding; protein binding; protein domain specific binding; transcription factor activity

Biological Process: BMP signaling pathway; cell maturation; chondrocyte development; chondrocyte differentiation; embryonic cranial skeleton morphogenesis; embryonic forelimb morphogenesis; endochondral ossification; gene expression; hemopoiesis; negative regulation of smoothened signaling pathway; negative regulation of transcription, DNA-dependent; neuron differentiation; odontogenesis of dentine-containing teeth; ossification; osteoblast development; osteoblast differentiation; osteoblast fate commitment; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription, DNA-dependent; regulation of cell differentiation; regulation of fibroblast growth factor receptor signaling pathway; regulation of odontogenesis of dentine-containing teeth; regulation of transcription from RNA polymerase II promoter; stem cell differentiation; T cell differentiation; transcription initiation from RNA polymerase II promoter

Disease: Cleidocranial Dysplasia; Metaphyseal Dysplasia With Maxillary Hypoplasia With Or Without Brachydactyly
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 Research Articles on RUNX2    1. A family with an affected mother and three affected children with isolated oligo/hypodontia caused by RUNX2 duplication has been described.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RUNX2 elisa kitPathways associated with RUNX2 elisa kit
 Reference Product  PubMed Publications
 BMP2 elisa kit  >455 publications with RUNX2 and BMP2
 SOX9 elisa kit  >276 publications with RUNX2 and SOX9
 SP7 elisa kit  >164 publications with RUNX2 and SP7
 BGLAP elisa kit  >57 publications with RUNX2 and BGLAP
 SMAD3 elisa kit  >47 publications with RUNX2 and SMAD3
 SMAD5 elisa kit  >42 publications with RUNX2 and SMAD5
 SMAD4 elisa kit  >32 publications with RUNX2 and SMAD4
 HES1 elisa kit  >17 publications with RUNX2 and HES1
 EP300 elisa kit  >7 publications with RUNX2 and EP300
 UBC elisa kit  >2 publications with RUNX2 and UBC
 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 BMP Signalling And Regulation Pathway antibodies  BMP Signalling And Regulation Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Interleukin-11 Signaling Pathway antibodies  Interleukin-11 Signaling Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
 Regulation Of Nuclear SMAD2/3 Signaling Pathway antibodies  Regulation Of Nuclear SMAD2/3 Signaling Pathway Diagram
 Regulation Of Retinoblastoma Protein Pathway antibodies  Regulation Of Retinoblastoma Protein Pathway Diagram
Diseases associated with RUNX2 elisa kitOrgans/Tissues associated with RUNX2 elisa kit
 Disease Name  Pubmed Publications
 Cleidocranial Dysplasia Antibodies  >175 publications with RUNX2 and Cleidocranial Dysplasia
 Craniofacial Abnormalities Antibodies  >170 publications with RUNX2 and Craniofacial Abnormalities
 Necrosis Antibodies  >133 publications with RUNX2 and Necrosis
 Inflammation Antibodies  >128 publications with RUNX2 and Inflammation
 Hypertrophy Antibodies  >122 publications with RUNX2 and Hypertrophy
 Osteosarcoma Antibodies  >117 publications with RUNX2 and Osteosarcoma
 Cardiovascular Diseases Antibodies  >113 publications with RUNX2 and Cardiovascular Diseases
 Osteoarthritis Antibodies  >105 publications with RUNX2 and Osteoarthritis
 Kidney Diseases Antibodies  >58 publications with RUNX2 and Kidney Diseases
 Breast Neoplasms Antibodies  >56 publications with RUNX2 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >3236 publications with RUNX2 and Bone
 Bone Marrow Antibodies  >762 publications with RUNX2 and Bone Marrow
 Mouth Antibodies  >288 publications with RUNX2 and Mouth
 Muscle Antibodies  >256 publications with RUNX2 and Muscle
 Embryonic Tissue Antibodies  >231 publications with RUNX2 and Embryonic Tissue
 Heart Antibodies  >71 publications with RUNX2 and Heart
 Brain Antibodies  >51 publications with RUNX2 and Brain
 Liver Antibodies  >47 publications with RUNX2 and Liver
 Lung Antibodies  >40 publications with RUNX2 and Lung
 Skin Antibodies  >38 publications with RUNX2 and Skin
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