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S100A12 elisa kit :: Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS455912
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $360 +1 FREE 8GB USB
96-Strip-Wells  /  $470 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,935 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

S100 Calcium Binding Protein A12 (S100A12), ELISA Kit

 Also Known As   

Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit

 Product Synonym Names    CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6
 Product Gene Name   

S100A12 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    D49549 mRNA
 3D Structure    ModBase 3D Structure for P80511
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of S100A12. No significant cross-reactivity or interference between S100A12 and analogues was observed.
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 Samples    Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    78.1-5000pg/mL
 Sensitivity    <34pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100A12 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100A12 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of S100A12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for S100A12 purchase    MBS455912 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S100 Calcium Binding Protein A12 (S100A12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78.1-5000pg/mL in biological research samples containing S100A12, with an estimated sensitivity of <34pg/mL. The ELISA analytical biochemical technique of the MBS455912 kit is based on S100A12 antibody-S100A12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S100A12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S100A12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for S100A12 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100A12 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100A12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to S100A12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100A12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100A12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of S100A12 elisa kit    S100A12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS455912
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NCBI/Uniprot data below describe general gene information for S100A12. It may not necessarily be applicable to this product.
 NCBI GI #    5032059
 NCBI GeneID    6283
 NCBI Accession #    NP_005612.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005621.1 [Other Products]
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 UniProt Primary Accession #    P80511 [Other Products]
 UniProt Secondary Accession #    P83219; Q5SY66; Q7M4R1 [Other Products]
 UniProt Related Accession #    P80511 [Other Products]
 Molecular Weight    10,575 Da
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 NCBI Official Full Name    protein S100-A12
 NCBI Official Synonym Full Names    S100 calcium binding protein A12
 NCBI Official Symbol    S100A12 [Similar Products]
 NCBI Official Synonym Symbols   
p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
[Similar Products]
 NCBI Protein Information    protein S100-A12
 UniProt Protein Name    Protein S100-A12
 UniProt Synonym Protein Names   
CGRP; Calcium-binding protein in amniotic fluid 1; CAAF1
 Protein Family    Protein
 UniProt Gene Name    S100A12 [Similar Products]
 UniProt Synonym Gene Names    CAAF1; CAGC; EN-RAGE; MRP-6; p6 [Similar Products]
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 NCBI Summary for S100A12    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014]
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 UniProt Comments for S100A12    S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn2+ from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.
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 Research Articles on S100A12    1. Findings suggest that S100 proteins S100A12 and S100A8/A9 are secreted independently either via distinct mechanisms of secretion or following the activation of different signal transduction pathways.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with S100A12 elisa kitPathways associated with S100A12 elisa kit
 Reference Product  PubMed Publications
 HMGB1 elisa kit  >23 publications with S100A12 and HMGB1
 S100A7 elisa kit  >15 publications with S100A12 and S100A7
 AGER elisa kit  >8 publications with S100A12 and AGER
 CACYBP elisa kit  >2 publications with S100A12 and CACYBP
 NOX1 elisa kit  >1 publications with S100A12 and NOX1
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 MyD88 Cascade Initiated On Plasma Membrane Pathway antibodies  MyD88 Cascade Initiated On Plasma Membrane Pathway Diagram
 MyD88 Dependent Cascade Initiated On Endosome Pathway antibodies  MyD88 Dependent Cascade Initiated On Endosome Pathway Diagram
 MyD88-independent TLR3/TLR4 Cascade Pathway antibodies  MyD88-independent TLR3/TLR4 Cascade Pathway Diagram
 MyD88:Mal Cascade Initiated On Plasma Membrane Pathway antibodies  MyD88:Mal Cascade Initiated On Plasma Membrane Pathway Diagram
Diseases associated with S100A12 elisa kitOrgans/Tissues associated with S100A12 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >225 publications with S100A12 and Inflammation
 Immune System Diseases Antibodies  >64 publications with S100A12 and Immune System Diseases
 Cardiovascular Diseases Antibodies  >61 publications with S100A12 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >51 publications with S100A12 and Vascular Diseases
 Necrosis Antibodies  >28 publications with S100A12 and Necrosis
 Atherosclerosis Antibodies  >26 publications with S100A12 and Atherosclerosis
 Colitis Antibodies  >23 publications with S100A12 and Colitis
 Nervous System Diseases Antibodies  >21 publications with S100A12 and Nervous System Diseases
 Diabetes Mellitus Antibodies  >18 publications with S100A12 and Diabetes Mellitus
 Kidney Diseases Antibodies  >17 publications with S100A12 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >207 publications with S100A12 and Blood
 Kidney Antibodies  >20 publications with S100A12 and Kidney
 Intestine Antibodies  >19 publications with S100A12 and Intestine
 Heart Antibodies  >11 publications with S100A12 and Heart
 Liver Antibodies  >8 publications with S100A12 and Liver
 Adipose Tissue Antibodies  >3 publications with S100A12 and Adipose Tissue
 Spleen Antibodies  >3 publications with S100A12 and Spleen
 Bone Marrow Antibodies  >3 publications with S100A12 and Bone Marrow
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