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S100A4 elisa kit :: Human S100 calcium binding protein A4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS915435
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

S100 calcium binding protein A4 (S100A4), ELISA Kit

★Popular Item★
 Also Known As   

Human Protein S100-A4, S100A4 ELISA Kit

 Product Synonym Names    Human Protein S100-A4 (S100A4) ELISA kit; 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; OTTHUMP00000015469; OTTHUMP00000032895; S100 calcium binding protein A4 (calcium protein; calvasculin; metastasin; murine placental homolog) ; S100 calcium-bind; S100 calcium binding protein A4
 Product Gene Name   

S100A4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS915435 Testing Data    Testing Data PDF
 OMIM    114210
 3D Structure    ModBase 3D Structure for P26447
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human S100A4. No significant cross-reactivity or interference between human S100A4 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    0.9 ng/ml-60 ng/ml.
 Sensitivity    0.225 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of S100A4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for S100A4 purchase    MBS915435 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S100 calcium binding protein A4 (S100A4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.9 ng/ml-60 ng/ml in biological research samples containing S100A4, with an estimated sensitivity of 0.225 ng/ml. The ELISA analytical biochemical technique of the MBS915435 kit is based on S100A4 antibody-S100A4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S100A4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S100A4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for S100A4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of S100A4 elisa kit    S100A4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS915435. Click to request current manual
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NCBI/Uniprot data below describe general gene information for S100A4. It may not necessarily be applicable to this product.
 NCBI GI #    4506765
 NCBI GeneID    6275
 NCBI Accession #    NP_002952.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002961.2 [Other Products]
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 UniProt Primary Accession #    P26447 [Other Products]
 UniProt Secondary Accession #    Q6ICP8; A8K7R8; D3DV46 [Other Products]
 UniProt Related Accession #    P26447 [Other Products]
 Molecular Weight    11,729 Da
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 NCBI Official Full Name    protein S100-A4
 NCBI Official Synonym Full Names    S100 calcium binding protein A4
 NCBI Official Symbol    S100A4 [Similar Products]
 NCBI Official Synonym Symbols   
42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
[Similar Products]
 NCBI Protein Information    protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
 UniProt Protein Name    Protein S100-A4
 UniProt Synonym Protein Names   
Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4
 Protein Family    Protein
 UniProt Gene Name    S100A4 [Similar Products]
 UniProt Synonym Gene Names    CAPL; MTS1 [Similar Products]
 UniProt Entry Name    S10A4_HUMAN
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 NCBI Summary for S100A4    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
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 UniProt Comments for S100A4    S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; neuron projection; perinuclear region of cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; RAGE receptor binding; calcium ion binding; actin binding; calcium-dependent protein binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; epithelial to mesenchymal transition
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 Research Articles on S100A4    1. S100A4 in endothelial cells is involved in tube formation, and suggest its potential as a molecular target for inhibiting tumor angiogenesis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with S100A4 elisa kitDiseases associated with S100A4 elisa kit
 Reference Product  PubMed Publications
 S100A1 elisa kit  >35 publications with S100A4 and S100A1
 S100P elisa kit  >22 publications with S100A4 and S100P
 TP53 elisa kit  >9 publications with S100A4 and TP53
 MYH9 elisa kit  >5 publications with S100A4 and MYH9
 NOV elisa kit  >3 publications with S100A4 and NOV
 METAP2 elisa kit  >2 publications with S100A4 and METAP2
 ANXA2 elisa kit  >2 publications with S100A4 and ANXA2
 SEPT6 elisa kit  >1 publications with S100A4 and SEPT6
 SEPT2 elisa kit  >1 publications with S100A4 and SEPT2
 PPFIBP1 elisa kit  >1 publications with S100A4 and PPFIBP1
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >366 publications with S100A4 and Neoplasms
 Carcinoma Antibodies  >186 publications with S100A4 and Carcinoma
 Neoplasm Metastasis Antibodies  >180 publications with S100A4 and Neoplasm Metastasis
 Adenocarcinoma Antibodies  >90 publications with S100A4 and Adenocarcinoma
 Breast Neoplasms Antibodies  >73 publications with S100A4 and Breast Neoplasms
 Skin Diseases Antibodies  >70 publications with S100A4 and Skin Diseases
 Disease Models, Animal Antibodies  >69 publications with S100A4 and Disease Models, Animal
 Fibrosis Antibodies  >67 publications with S100A4 and Fibrosis
 Lung Diseases Antibodies  >54 publications with S100A4 and Lung Diseases
 Neoplasms, Experimental Antibodies  >47 publications with S100A4 and Neoplasms, Experimental
Organs/Tissues associated with S100A4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >95 publications with S100A4 and Muscle
 Vascular Antibodies  >77 publications with S100A4 and Vascular
 Blood Antibodies  >76 publications with S100A4 and Blood
 Lung Antibodies  >65 publications with S100A4 and Lung
 Bone Antibodies  >54 publications with S100A4 and Bone
 Kidney Antibodies  >50 publications with S100A4 and Kidney
 Lymph Node Antibodies  >46 publications with S100A4 and Lymph Node
 Liver Antibodies  >46 publications with S100A4 and Liver
 Embryonic Tissue Antibodies  >34 publications with S100A4 and Embryonic Tissue
 Brain Antibodies  >32 publications with S100A4 and Brain
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