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SFRP5 elisa kit :: Human SFRP5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS8248400
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $490 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SFRP5, ELISA Kit

★Popular Item★
 Also Known As   

Human SFRP5 ELISA Kit

 Product Synonym Names    FRP1B; SARP3; Secreted frizzled-related protein 5; sFRP-5; Frizzled-related protein 1b; FRP-1b; Secreted apoptosis-related protein 3; SARP-3
 Product Gene Name   

SFRP5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    AF017988 mRNA
 3D Structure    ModBase 3D Structure for Q5T4F7
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 Species Reactivity    Human
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 Specificity    The Human SFRP5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human SFRP5 proteins within the range of 156 pg/ml - 10000 pg/ml.
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 Samples    Cell Culture Supernates, Serum, Plasma
 Sensitivity    <15 pg/mL
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SFRP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SFRP5 purchase    MBS8248400 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SFRP5, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SFRP5, with an estimated sensitivity of <15 pg/mL. The ELISA analytical biochemical technique of the MBS8248400 kit is based on SFRP5 antibody-SFRP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SFRP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SFRP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernates, Serum, Plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SFRP5 elisa kit

   Principle of the Assay: MDS Human SFRP5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SFRP5 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human SFRP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and SFRP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SFRP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SFRP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
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 Typical Testing Data/Standard Curve (for reference only) of SFRP5 elisa kit    SFRP5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS8248400. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SFRP5. It may not necessarily be applicable to this product.
 NCBI GI #    188528609
 NCBI GeneID    6425
 NCBI Accession #    NP_003006.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003015.3 [Other Products]
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 UniProt Primary Accession #    Q5T4F7 [Other Products]
 UniProt Secondary Accession #    O14780; Q86TH7 [Other Products]
 UniProt Related Accession #    Q5T4F7 [Other Products]
 Molecular Weight    35,563 Da
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 NCBI Official Full Name    secreted frizzled-related protein 5
 NCBI Official Synonym Full Names    secreted frizzled related protein 5
 NCBI Official Symbol    SFRP5 [Similar Products]
 NCBI Official Synonym Symbols   
SARP3
[Similar Products]
 NCBI Protein Information    secreted frizzled-related protein 5
 UniProt Protein Name    Secreted frizzled-related protein 5
 UniProt Synonym Protein Names   
Frizzled-related protein 1b; FRP-1b; Secreted apoptosis-related protein 3; SARP-3
 Protein Family    Secreted frizzled-related protein
 UniProt Gene Name    SFRP5 [Similar Products]
 UniProt Synonym Gene Names    FRP1B; SARP3; sFRP-5; FRP-1b; SARP-3 [Similar Products]
 UniProt Entry Name    SFRP5_HUMAN
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 NCBI Summary for SFRP5    Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SFRP5    SFRP5: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: extracellular space; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt receptor activity; Wnt-protein binding

Biological Process: anatomical structure morphogenesis; apoptosis; establishment and/or maintenance of cell polarity; negative regulation of cell proliferation; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; signal transduction; visual perception
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 Research Articles on SFRP5    1. Decreased first trimester serum Sfrp-5 levels are significantly associated with the increased risk of gestational diabetes mellitus.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SFRP5 elisa kitPathways associated with SFRP5 elisa kit
 Reference Product  PubMed Publications
 WNT5A elisa kit  >24 publications with SFRP5 and WNT5A
 WNT3A elisa kit  >6 publications with SFRP5 and WNT3A
 WNT4 elisa kit  >4 publications with SFRP5 and WNT4
 WNT2B elisa kit  >3 publications with SFRP5 and WNT2B
 WNT10B elisa kit  >3 publications with SFRP5 and WNT10B
 WNT8A elisa kit  >3 publications with SFRP5 and WNT8A
 WNT11 elisa kit  >3 publications with SFRP5 and WNT11
 LHX5 elisa kit  >1 publications with SFRP5 and LHX5
 Products by Pathway  Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
Diseases associated with SFRP5 elisa kitOrgans/Tissues associated with SFRP5 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >43 publications with SFRP5 and Carcinoma
 Inflammation Antibodies  >21 publications with SFRP5 and Inflammation
 Colorectal Neoplasms Antibodies  >20 publications with SFRP5 and Colorectal Neoplasms
 Colonic Neoplasms Antibodies  >11 publications with SFRP5 and Colonic Neoplasms
 Diabetes Mellitus, Type 2 Antibodies  >10 publications with SFRP5 and Diabetes Mellitus, Type 2
 Carcinoma, Hepatocellular Antibodies  >8 publications with SFRP5 and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >8 publications with SFRP5 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >7 publications with SFRP5 and Breast Neoplasms
 Fatty Liver Antibodies  >6 publications with SFRP5 and Fatty Liver
 Necrosis Antibodies  >6 publications with SFRP5 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >69 publications with SFRP5 and Eye
 Intestine Antibodies  >9 publications with SFRP5 and Intestine
 Brain Antibodies  >7 publications with SFRP5 and Brain
 Pancreas Antibodies  >5 publications with SFRP5 and Pancreas
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