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SMARCA4 elisa kit :: Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS909083
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), ELISA Kit

 Also Known As   

Human Transcription activator BRG1, SMARCA4 ELISA Kit

 Product Synonym Names    Human Transcription activator BRG1 (SMARCA4) ELISA kit; BAF190; BRG1; FLJ39786; SNF2; SNF2-BETA; SNF2L4; SNF2LB; SWI2; hSNF2b; ATP-dependent helicase SMARCA4; BRM/SWI2-related gene 1; SNF2-like 4; SWI/SNF-related matrix-associated actin-dependent regulator; SWI/SNF related; matrix associated; actin dependent regulator of chromatin; subfamily a; member 4
 Product Gene Name   

SMARCA4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS909083 Testing Data    Testing Data PDF
 OMIM    614609
 3D Structure    ModBase 3D Structure for P51532
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human SMARCA4. No significant cross-reactivity or interference between human SMARCA4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    25 pg/ml -1600 pg/ml.
 Sensitivity    The minimum detectable dose of human SMARCA4 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SMARCA4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SMARCA4 purchase    MBS909083 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml -1600 pg/ml in biological research samples containing SMARCA4, with an estimated sensitivity of The minimum detectable dose of human SMARCA4 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS909083 kit is based on SMARCA4 antibody-SMARCA4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SMARCA4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SMARCA4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SMARCA4 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SMARCA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SMARCA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMARCA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMARCA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SMARCA4 elisa kit    SMARCA4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS909083. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SMARCA4. It may not necessarily be applicable to this product.
 NCBI GI #    192807312
 NCBI GeneID    6597
 NCBI Accession #    NP_001122316.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001128844.1 [Other Products]
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 UniProt Primary Accession #    P51532 [Other Products]
 UniProt Secondary Accession #    O95052; Q9HBD3; B1A8Z4; B1A8Z5; B1A8Z6; B1A8Z7; E9PBR8 [Other Products]
 UniProt Related Accession #    P51532 [Other Products]
 Molecular Weight    184,646 Da
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 NCBI Official Full Name    transcription activator BRG1 isoform B
 NCBI Official Synonym Full Names    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
 NCBI Official Symbol    SMARCA4 [Similar Products]
 NCBI Official Synonym Symbols   
BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
[Similar Products]
 NCBI Protein Information    transcription activator BRG1; SNF2-beta; SNF2-like 4; protein BRG-1; nuclear protein GRB1; brahma protein-like 1; BRM/SWI2-related gene 1; homeotic gene regulator; protein brahma homolog 1; BRG1-associated factor 190A; sucrose nonfermenting-like 4; ATP-dependent helicase SMARCA4; mitotic growth and transcription activator; global transcription activator homologous sequence
 UniProt Protein Name    Transcription activator BRG1
 UniProt Synonym Protein Names   
ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
 Protein Family    Transcription activator
 UniProt Gene Name    SMARCA4 [Similar Products]
 UniProt Synonym Gene Names    BAF190A; BRG1; SNF2B; SNF2L4; BAF190A [Similar Products]
 UniProt Entry Name    SMCA4_HUMAN
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 NCBI Summary for SMARCA4    The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
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 UniProt Comments for SMARCA4    SMARCA4: a member of the SWI/SNF nucleosome remodeling complex with ATP-dependent DNA helicase activity. SWI/SNF complexes are required for mammalian development and are mutated in ~20% of all human primary tumors. Mutated in 35% (13/37) of NSCLC and 5% (1/19) of SCLC human lung cancer cell lines . A component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. Belongs to the neural progenitor-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). Component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Transcriptional coactivator of nuclear hormone receptors. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription, and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. SMARCA4, in consort with ZEB1, represses the transcription of E-cadherin and promotes the epithelial-to-mesenchymal transisition (EMT). SMARCA4 is required for stem cell maintenance in the mouse intestinal epithelium. Its loss inhibits aberrant Wnt-signalling and prevents tumorigenesis in the mouse small intestine. SMARCA4 mutations are the cause of a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood. Belongs to the SNF2/RAD54 helicase family. The human protein includes 5 isoforms produced by alternative splicing.

Protein type: Cell cycle regulation; Helicase; EC 3.6.1.-; Tumor suppressor; Transcription, coactivator/corepressor; EC 3.6.4.-; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; heterochromatin; SWI/SNF complex; extracellular space; protein complex; membrane; perichromatin fibrils; nuclear chromatin; nucleolus; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; nucleosomal DNA binding; androgen receptor binding; Tat protein binding; p53 binding; transcription coactivator activity; protein N-terminus binding; transcription corepressor activity; helicase activity; ATP binding

Biological Process: extracellular matrix organization and biogenesis; establishment and/or maintenance of chromatin architecture; glial cell fate determination; negative regulation of transcription from RNA polymerase II promoter, mitotic; positive regulation of transcription, DNA-dependent; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; embryonic organ morphogenesis; epidermis morphogenesis; blastocyst hatching; forebrain development; blastocyst growth; vasculogenesis; positive regulation of Wnt receptor signaling pathway; positive regulation of DNA binding; hindbrain development; transcription, DNA-dependent; stem cell maintenance; liver development; regulation of transcription from RNA polymerase II promoter; keratinocyte differentiation; chromatin remodeling; cytosine methylation within a CG sequence; methylation-dependent chromatin silencing; nucleosome disassembly; ATP-dependent chromatin remodeling; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent

Disease: Mental Retardation, Autosomal Dominant 16; Rhabdoid Tumor Predisposition Syndrome 2
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 Research Articles on SMARCA4    1. SMARCA4 rs11879293 presented consistently significant associations with the risk of hepatocellular carcinoma
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SMARCA4 elisa kitPathways associated with SMARCA4 elisa kit
 Reference Product  PubMed Publications
 SMARCB1 elisa kit  >29 publications with SMARCA4 and SMARCB1
 SMARCC1 elisa kit  >17 publications with SMARCA4 and SMARCC1
 ARID1A elisa kit  >16 publications with SMARCA4 and ARID1A
 SMARCE1 elisa kit  >9 publications with SMARCA4 and SMARCE1
 BRCA1 elisa kit  >8 publications with SMARCA4 and BRCA1
 CTNNB1 elisa kit  >8 publications with SMARCA4 and CTNNB1
 CDKN1A elisa kit  >7 publications with SMARCA4 and CDKN1A
 ARID1B elisa kit  >5 publications with SMARCA4 and ARID1B
 ACTL6A elisa kit  >4 publications with SMARCA4 and ACTL6A
 PAX6 elisa kit  >4 publications with SMARCA4 and PAX6
 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 Regulation Of Retinoblastoma Protein Pathway antibodies  Regulation Of Retinoblastoma Protein Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram
Diseases associated with SMARCA4 elisa kitOrgans/Tissues associated with SMARCA4 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >100 publications with SMARCA4 and Neoplasms
 Carcinoma Antibodies  >37 publications with SMARCA4 and Carcinoma
 Adenocarcinoma Antibodies  >15 publications with SMARCA4 and Adenocarcinoma
 Lung Neoplasms Antibodies  >14 publications with SMARCA4 and Lung Neoplasms
 Leukemia Antibodies  >13 publications with SMARCA4 and Leukemia
 Breast Neoplasms Antibodies  >13 publications with SMARCA4 and Breast Neoplasms
 Nervous System Diseases Antibodies  >12 publications with SMARCA4 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >11 publications with SMARCA4 and Cardiovascular Diseases
 Death Antibodies  >11 publications with SMARCA4 and Death
 Cell Transformation, Neoplastic Antibodies  >10 publications with SMARCA4 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >42 publications with SMARCA4 and Blood
 Embryonic Tissue Antibodies  >25 publications with SMARCA4 and Embryonic Tissue
 Lung Antibodies  >23 publications with SMARCA4 and Lung
 Brain Antibodies  >23 publications with SMARCA4 and Brain
 Muscle Antibodies  >21 publications with SMARCA4 and Muscle
 Heart Antibodies  >18 publications with SMARCA4 and Heart
 Prostate Antibodies  >11 publications with SMARCA4 and Prostate
 Liver Antibodies  >10 publications with SMARCA4 and Liver
 Testis Antibodies  >8 publications with SMARCA4 and Testis
 Vascular Antibodies  >8 publications with SMARCA4 and Vascular
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