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SEMA4D elisa kit :: Human Semaphorin 4D (SEMA4D) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2703296 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $210 +1 FREE 8GB USB
48-Strip-Wells  /  $345 +1 FREE 8GB USB
96-Strip-Wells  /  $460 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,910 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,530 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Semaphorin 4D (SEMA4D), ELISA Kit

★Popular Item★
 Also Known As   

Human Semaphorin 4D (SEMA4D) ELISA Kit

 Product Synonym Names    CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D
 Product Gene Name   

SEMA4D elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    U60800 mRNA
 3D Structure    ModBase 3D Structure for Q92854
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Semaphorin 4D (SEMA4D).
No significant cross-reactivity or interference between Semaphorin 4D (SEMA4D) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
 Detection Range    1.56-100ng/mL
 Sensitivity    < 13.5pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SEMA4D elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SEMA4D purchase    MBS2703296 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Semaphorin 4D (SEMA4D) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56-100ng/mL in biological research samples containing SEMA4D, with an estimated sensitivity of < 13.5pg/mL. The ELISA analytical biochemical technique of the MBS2703296 kit is based on SEMA4D antibody-SEMA4D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA4D antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA4D. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SEMA4D elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4D (SEMA4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4D (SEMA4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4D (SEMA4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 4D (SEMA4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of SEMA4D elisa kit    SEMA4D elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2703296. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SEMA4D. It may not necessarily be applicable to this product.
 NCBI GI #    214010218
 NCBI GeneID    10507
 NCBI Accession #    NP_006369.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_006378.3 [Other Products]
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 UniProt Primary Accession #    Q92854 [Other Products]
 UniProt Secondary Accession #    Q7Z5S4; Q8N8B0; B2RPM6 [Other Products]
 UniProt Related Accession #    Q92854 [Other Products]
 Molecular Weight    82,164 Da
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 NCBI Official Full Name    semaphorin-4D isoform 1
 NCBI Official Synonym Full Names    semaphorin 4D
 NCBI Official Symbol    SEMA4D [Similar Products]
 NCBI Official Synonym Symbols   
CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G
[Similar Products]
 NCBI Protein Information    semaphorin-4D
 UniProt Protein Name    Semaphorin-4D
 UniProt Synonym Protein Names   
A8; BB18; GR3; CD_antigen: CD100
 Protein Family    Semaphorin
 UniProt Gene Name    SEMA4D [Similar Products]
 UniProt Synonym Gene Names    C9orf164; CD100; SEMAJ [Similar Products]
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 UniProt Comments for SEMA4D    SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral component of plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane receptor activity

Biological Process: cell adhesion; immune response; negative chemotaxis; negative regulation of apoptosis; negative regulation of cell adhesion; negative regulation of osteoblast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell migration; positive regulation of collateral sprouting; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein phosphorylation; regulation of cell projection organization; regulation of cell shape; regulation of dendrite morphogenesis
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 Research Articles on SEMA4D    1. The positive expression of both Sema4D and PlexinB1 was found to be an independent risk factor for a worse survival in colorectal cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SEMA4D elisa kitPathways associated with SEMA4D elisa kit
 Reference Product  PubMed Publications
 PLXNB1 elisa kit  >57 publications with SEMA4D and PLXNB1
 CD72 elisa kit  >31 publications with SEMA4D and CD72
 MET elisa kit  >17 publications with SEMA4D and MET
 ERBB2 elisa kit  >6 publications with SEMA4D and ERBB2
 PTPRC elisa kit  >5 publications with SEMA4D and PTPRC
 RND1 elisa kit  >4 publications with SEMA4D and RND1
 RRAS elisa kit  >3 publications with SEMA4D and RRAS
 ARHGEF12 elisa kit  >2 publications with SEMA4D and ARHGEF12
 ARHGEF11 elisa kit  >2 publications with SEMA4D and ARHGEF11
 GRLF1 elisa kit  >1 publications with SEMA4D and GRLF1
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Other Semaphorin Interactions Pathway antibodies  Other Semaphorin Interactions Pathway Diagram
 Sema4D In Semaphorin Signaling Pathway antibodies  Sema4D In Semaphorin Signaling Pathway Diagram
 Sema4D Induced Cell Migration And Growth-cone Collapse Pathway antibodies  Sema4D Induced Cell Migration And Growth-cone Collapse Pathway Diagram
 Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway antibodies  Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway Diagram
 Semaphorin Interactions Pathway antibodies  Semaphorin Interactions Pathway Diagram
Diseases associated with SEMA4D elisa kitOrgans/Tissues associated with SEMA4D elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >31 publications with SEMA4D and Neoplasms
 Nervous System Diseases Antibodies  >19 publications with SEMA4D and Nervous System Diseases
 Brain Diseases Antibodies  >9 publications with SEMA4D and Brain Diseases
 Inflammation Antibodies  >8 publications with SEMA4D and Inflammation
 Disease Models, Animal Antibodies  >6 publications with SEMA4D and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >6 publications with SEMA4D and Cardiovascular Diseases
 Kidney Diseases Antibodies  >4 publications with SEMA4D and Kidney Diseases
 Necrosis Antibodies  >3 publications with SEMA4D and Necrosis
 Nerve Degeneration Antibodies  >2 publications with SEMA4D and Nerve Degeneration
 Cerebral Infarction Antibodies  >2 publications with SEMA4D and Cerebral Infarction
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >83 publications with SEMA4D and Nerve
 Blood Antibodies  >64 publications with SEMA4D and Blood
 Brain Antibodies  >22 publications with SEMA4D and Brain
 Embryonic Tissue Antibodies  >15 publications with SEMA4D and Embryonic Tissue
 Kidney Antibodies  >7 publications with SEMA4D and Kidney
 Skin Antibodies  >5 publications with SEMA4D and Skin
 Lymph Node Antibodies  >5 publications with SEMA4D and Lymph Node
 Lung Antibodies  >4 publications with SEMA4D and Lung
 Bone Marrow Antibodies  >4 publications with SEMA4D and Bone Marrow
 Spinal Cord Antibodies  >4 publications with SEMA4D and Spinal Cord
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