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PLK4 elisa kit :: Human Serine/threonine-protein kinase PLK4, PLK4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2602717
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Serine/threonine-protein kinase PLK4, PLK4, ELISA Kit

★Popular Item★
 Also Known As   

Human Serine/threonine-protein kinase PLK4, PLK4 ELISA Kit

 Product Gene Name   

PLK4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605031
 3D Structure    ModBase 3D Structure for O00444
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PLK4 concentration
 Assay Type    Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PLK4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PLK4 purchase    MBS2602717 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Serine/threonine-protein kinase PLK4, PLK4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing PLK4, with an estimated sensitivity of 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS2602717 kit is based on PLK4 antibody-PLK4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLK4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLK4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PLK4 concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PLK4 elisa kit

   Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PLK4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of PLK4 elisa kit    PLK4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2602717. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PLK4. It may not necessarily be applicable to this product.
 NCBI GI #    299829210
 NCBI GeneID    10733
 NCBI Accession #    NP_001177728.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001190799.1 [Other Products]
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 UniProt Primary Accession #    O00444 [Other Products]
 UniProt Secondary Accession #    Q8IYF0; Q96Q95; Q9UD84; Q9UDE2; B2RAL0; B7Z837; B7Z8G7 [Other Products]
 UniProt Related Accession #    O00444 [Other Products]
 Molecular Weight    108,972 Da
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 NCBI Official Full Name    serine/threonine-protein kinase PLK4 isoform 2
 NCBI Official Synonym Full Names    polo-like kinase 4
 NCBI Official Symbol    PLK4 [Similar Products]
 NCBI Official Synonym Symbols   
SAK; STK18
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase PLK4; PLK-4; Snk akin kinase; serine/threonine kinase 18; serine/threonine-protein kinase 18; serine/threonine protein kinase SAK; serine/threonine-protein kinase Sak
 UniProt Protein Name    Serine/threonine-protein kinase PLK4
 UniProt Synonym Protein Names   
Polo-like kinase 4; PLK-4; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase Sak
 UniProt Gene Name    PLK4 [Similar Products]
 UniProt Synonym Gene Names    SAK; STK18; PLK-4 [Similar Products]
 UniProt Entry Name    PLK4_HUMAN
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 NCBI Summary for PLK4    This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
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 UniProt Comments for PLK4    PLK4: a kinase of the PLK family. Localizes to centrosomes and the cleavage furrow during cytokinesis. Interacts with the protein kinase Tec.

Protein type: Kinase, protein; Nucleolus; Protein kinase, Other; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: centriole; centrosome; nucleolus; cytosol; cleavage furrow

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: organelle organization and biogenesis; positive regulation of centriole replication; centriole replication; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation

Disease: Microcephaly And Chorioretinopathy, Autosomal Recessive, 2
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 Research Articles on PLK4    1. PLK4 is a new NFkappaB target gene, providing a direct link between NFkappaB activity and centrosome duplication, with implications for the role of these transcription factors in tumorigenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PLK4 elisa kitPathways associated with PLK4 elisa kit
 Reference Product  PubMed Publications
 TEC elisa kit  >1 publications with PLK4 and TEC
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram
 G2/M Transition Pathway antibodies  G2/M Transition Pathway Diagram
 Loss Of Nlp From Mitotic Centrosomes Pathway antibodies  Loss Of Nlp From Mitotic Centrosomes Pathway Diagram
 Loss Of Proteins Required For Interphase Microtubule Organization From The Centrosome Pathway antibodies  Loss Of Proteins Required For Interphase Microtubule Organization From The Centrosome Pathway Diagram
 Mitotic G2-G2/M Phases Pathway antibodies  Mitotic G2-G2/M Phases Pathway Diagram
 PLK2 And PLK4 Events Pathway antibodies  PLK2 And PLK4 Events Pathway Diagram
 Polo-like Kinase Signaling Events In The Cell Cycle Pathway antibodies  Polo-like Kinase Signaling Events In The Cell Cycle Pathway Diagram
 Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway antibodies  Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway Diagram
Diseases associated with PLK4 elisa kitOrgans/Tissues associated with PLK4 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >20 publications with PLK4 and Neoplasms
 Liver Neoplasms Antibodies  >4 publications with PLK4 and Liver Neoplasms
 Nervous System Diseases Antibodies  >3 publications with PLK4 and Nervous System Diseases
 Skin Diseases Antibodies  >3 publications with PLK4 and Skin Diseases
 Liver Diseases Antibodies  >3 publications with PLK4 and Liver Diseases
 Breast Neoplasms Antibodies  >3 publications with PLK4 and Breast Neoplasms
 Lung Neoplasms Antibodies  >2 publications with PLK4 and Lung Neoplasms
 Carcinoma Antibodies  >2 publications with PLK4 and Carcinoma
 Adenocarcinoma Antibodies  >2 publications with PLK4 and Adenocarcinoma
 Brain Diseases Antibodies  >2 publications with PLK4 and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >5 publications with PLK4 and Embryonic Tissue
 Lung Antibodies  >4 publications with PLK4 and Lung
 Kidney Antibodies  >4 publications with PLK4 and Kidney
 Testis Antibodies  >4 publications with PLK4 and Testis
 Bone Antibodies  >2 publications with PLK4 and Bone
 Placenta Antibodies  >2 publications with PLK4 and Placenta
 Brain Antibodies  >2 publications with PLK4 and Brain
 Trachea Antibodies  >1 publications with PLK4 and Trachea
 Stomach Antibodies  >1 publications with PLK4 and Stomach
 Thymus Antibodies  >1 publications with PLK4 and Thymus
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