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SIRPa elisa kit :: Human Signal Regulatory Protein Alpha (SIRPa) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019546 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $255 +1 FREE 8GB USB
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $610 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Signal Regulatory Protein Alpha (SIRPa), ELISA Kit

★Popular Item★
 Also Known As   

Signal Regulatory Protein Alpha (SIRPa) ELISA Kit

 Product Synonym Names    CD172a; SIRP-A; P84; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; Macrophage fusion receptor
 Product Gene Name   

SIRPa elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602461
 3D Structure    ModBase 3D Structure for P78324
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Signal Regulatory Protein Alpha (SIRPa).
No significant cross-reactivity or interference between Signal Regulatory Protein Alpha (SIRPa) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.056ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Signal Regulatory Protein Alpha (SIRPa) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Signal Regulatory Protein Alpha (SIRPa) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SIRPa elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SIRPa purchase    MBS2019546 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Signal Regulatory Protein Alpha (SIRPa) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing SIRPa, with an estimated sensitivity of < 0.056ng/mL. The ELISA analytical biochemical technique of the MBS2019546 kit is based on SIRPa antibody-SIRPa antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SIRPa antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SIRPa. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SIRPa elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Alpha (SIRPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Alpha (SIRPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Alpha (SIRPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Regulatory Protein Alpha (SIRPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for SIRPa elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of SIRPa elisa kit    SIRPa elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019546. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SIRPa. It may not necessarily be applicable to this product.
 NCBI GI #    49899261
 NCBI GeneID    140885
 NCBI Accession #    AAH75849.1 [Other Products]
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 UniProt Primary Accession #    P78324 [Other Products]
 UniProt Secondary Accession #    O00683; O43799; Q8N517; Q8TAL8; Q9H0Z2; Q9UDX2; Q9UIJ6; Q9Y4U9; A2A2E1; A8K411; B2R6C3 [Other Products]
 UniProt Related Accession #    P78324 [Other Products]
 Molecular Weight    54,852 Da
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 NCBI Official Full Name    Signal-regulatory protein alpha
 NCBI Official Synonym Full Names    signal-regulatory protein alpha
 NCBI Official Symbol    SIRPA [Similar Products]
 NCBI Official Synonym Symbols   
BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
[Similar Products]
 NCBI Protein Information    tyrosine-protein phosphatase non-receptor type substrate 1
 UniProt Protein Name    Tyrosine-protein phosphatase non-receptor type substrate 1
 UniProt Synonym Protein Names   
Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84; CD_antigen: CD172a
 Protein Family    Tyrosine-protein phosphatase non-receptor
 UniProt Gene Name    SIRPA [Similar Products]
 UniProt Synonym Gene Names    BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3 [Similar Products]
 UniProt Entry Name    SHPS1_HUMAN
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 NCBI Summary for SIRPa    The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SIRPa    SHPS1: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Motility/polarity/chemotaxis; Cell adhesion; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: SH3 domain binding

Biological Process: cell adhesion; blood coagulation; leukocyte migration
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 Research Articles on SIRPa    1. Velcro" engineering of high affinity CD47 ectodomain as signal regulatory protein alpha (SIRPalpha) antagonists that enhance antibody-dependent cellular phagocytosis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SIRPa elisa kitDiseases associated with SIRPa elisa kit
 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL-1 Signaling Pathway antibodies  IL-1 Signaling Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Prolactin Signaling Pathway antibodies  Prolactin Signaling Pathway Diagram
 Signal Regulatory Protein (SIRP) Family Interactions Pathway antibodies  Signal Regulatory Protein (SIRP) Family Interactions Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >23 publications with SIRPa and Inflammation
 Cardiovascular Diseases Antibodies  >16 publications with SIRPa and Cardiovascular Diseases
 Diabetes Mellitus Antibodies  >15 publications with SIRPa and Diabetes Mellitus
 Breast Neoplasms Antibodies  >9 publications with SIRPa and Breast Neoplasms
 Disease Models, Animal Antibodies  >8 publications with SIRPa and Disease Models, Animal
 Respiratory Tract Diseases Antibodies  >8 publications with SIRPa and Respiratory Tract Diseases
 Necrosis Antibodies  >8 publications with SIRPa and Necrosis
 Lung Diseases Antibodies  >7 publications with SIRPa and Lung Diseases
 Kidney Diseases Antibodies  >5 publications with SIRPa and Kidney Diseases
 Infertility, Male Antibodies  >4 publications with SIRPa and Infertility, Male
Organs/Tissues associated with SIRPa elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >80 publications with SIRPa and Blood
 Vascular Antibodies  >22 publications with SIRPa and Vascular
 Bone Antibodies  >19 publications with SIRPa and Bone
 Muscle Antibodies  >18 publications with SIRPa and Muscle
 Prostate Antibodies  >13 publications with SIRPa and Prostate
 Eye Antibodies  >10 publications with SIRPa and Eye
 Lung Antibodies  >10 publications with SIRPa and Lung
 Heart Antibodies  >9 publications with SIRPa and Heart
 Bone Marrow Antibodies  >9 publications with SIRPa and Bone Marrow
 Skin Antibodies  >8 publications with SIRPa and Skin
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