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SPJ elisa kit :: Human Surfactant Protein J ELISA Kit

Scan QR to view Datasheet Catalog #    MBS720330
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $470 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $470 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $675 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,850 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,850 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,340 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,340 +6 FREE 8GB USB
 
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 Product Name   

Surfactant Protein J (SPJ), ELISA Kit

 Also Known As   

Human Surfactant Protein J ELISA Kit

 Product Gene Name   

SPJ elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Human
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 Assay Type    Competitive or Sandwich
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Sensitivity    0.1 ng/mL.
 Intended Uses    This SPJ ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SPJ. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of SPJ elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SPJ purchase    MBS720330 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Surfactant Protein J (SPJ) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPJ, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS720330 kit is based on SPJ antibody-SPJ antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPJ antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPJ. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for SPJ elisa kit

   Principle of the Assay: SPJ ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SPJ. Standards or samples are then added to the microtiter plate wells and SPJ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SPJ present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for SPJ are added to each well to "sandwich" the SPJ immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SPJ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPJ concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for SPJ elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of SPJ elisa kit    SPJ elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS720330
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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