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TAF elisa kit :: Human TAF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2504326
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TAF, ELISA Kit

★Popular Item★
 Also Known As   

Human TAF (TATA Box Binding Protein/TBP-Associated Factors) ELISA Kit

 Product Gene Name   

TAF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609514
 3D Structure    ModBase 3D Structure for Q7Z7C8
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human TAF. No significant cross-reactivity or interference between Human TAF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human TAF and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Min: 0.188ng/mL; Max: 20ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TAF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TAF purchase    MBS2504326 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TAF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing TAF, with an estimated sensitivity of Min: 0.188ng/mL; Max: 20ng/mL. The ELISA analytical biochemical technique of the MBS2504326 kit is based on TAF antibody-TAF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TAF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TAF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TAF elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TAF in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TAF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human TAF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human TAF. You can calculate the concentration of Human TAF in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TAF elisa kit    TAF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2504326. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TAF. It may not necessarily be applicable to this product.
 NCBI GI #    104485446
 NCBI GeneID    129685
 NCBI Accession #    NP_612639.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_138572.2 [Other Products]
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 UniProt Primary Accession #    Q7Z7C8 [Other Products]
 UniProt Secondary Accession #    Q5T0K1; Q8N4R9; Q96M52 [Other Products]
 UniProt Related Accession #    Q7Z7C8 [Other Products]
 Molecular Weight    37,411 Da
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 NCBI Official Full Name    transcription initiation factor TFIID subunit 8
 NCBI Official Synonym Full Names    TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa
 NCBI Official Symbol    TAF8 [Similar Products]
 NCBI Official Synonym Symbols   
43; II; TAF; TBN; TAFII43; TAFII-43
[Similar Products]
 NCBI Protein Information    transcription initiation factor TFIID subunit 8; TAF(II)43; TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 45/50kDa; TBP-associated factor 43 kDa; TBP-associated factor 8; TBP-associated factor TAFII43; TBP-associated factor, RNA polymerase II, 43 kD; hTAFII43; protein taube nuss; taube nuss homolog; transcription initiation factor TFIID 43 kDa subunit
 UniProt Protein Name    Transcription initiation factor TFIID subunit 8
 UniProt Synonym Protein Names   
Protein taube nuss; TBP-associated factor 43 kDa; TBP-associated factor 8; Transcription initiation factor TFIID 43 kDa subunit; TAFII-43; TAFII43; hTAFII43
 Protein Family    Tafazzin
 UniProt Gene Name    TAF8 [Similar Products]
 UniProt Synonym Gene Names    TAFII43; TBN; TAFII-43; TAFII43; hTAFII43 [Similar Products]
 UniProt Entry Name    TAF8_HUMAN
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 NCBI Summary for TAF    This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TAF    TBN: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo. Belongs to the TAF8 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; transcription factor TFIID complex; perinuclear region of cytoplasm; nucleus

Molecular Function: protein binding; protein heterodimerization activity

Biological Process: transcription, DNA-dependent; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; maintenance of protein localization in nucleus; cell differentiation; inner cell mass cell proliferation
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 Research Articles on TAF    1. Observational study of gene-disease association. (HuGE Navigator)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TAF elisa kitPathways associated with TAF elisa kit
 Reference Product  PubMed Publications
 TBP elisa kit  >7 publications with TAF and TBP
 TAF10 elisa kit  >4 publications with TAF and TAF10
 TAF4 elisa kit  >1 publications with TAF and TAF4
 TAF2 elisa kit  >1 publications with TAF and TAF2
 TAF12 elisa kit  >1 publications with TAF and TAF12
 TAF5 elisa kit  >1 publications with TAF and TAF5
 Products by Pathway  Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
Organs/Tissues associated with TAF elisa kit
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1 publications with TAF and Eye
 Salivary Gland Antibodies  >1 publications with TAF and Salivary Gland
 Prostate Antibodies  >1 publications with TAF and Prostate
 Mouth Antibodies  >1 publications with TAF and Mouth
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