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TGFA elisa kit :: Human TGF alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175792
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1x96 Wells  /  $440 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TGF alpha (TGFA), ELISA Kit

 Also Known As   

Human TGF alpha PicoKine ELISA Kit

 Product Synonym Names    Protransforming growth factor alpha; transforming growth factor, alpha; EGF like TGF; ETGF; OTTHUMP00000203300; OTTHUMP00000203301; OTTHUMP00000203345; OTTHUMP00000203352; Protransforming growth factor alpha; RATTGFAA; TFGA; TGF A; TGF type 1; TGFA; Transforming growth factor alpha; Transforming growth factor alpha precursor; Wa1; Waved 1
 Product Gene Name   

TGFA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    190170
 3D Structure    ModBase 3D Structure for P01135
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 Species Reactivity    Human
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 Specificity    Natural and recombinant human TGFalpha
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 Samples    Cell culture supernates, serum, plasma (heparin, EDTA) and human milk
 Assay Type    Sandwich
 Detection Range    15.6pg/ml-1000pg/ml
 Sensitivity    < 1pg/ml
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TGFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TGFA purchase    MBS175792 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TGF alpha (TGFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6pg/ml-1000pg/ml in biological research samples containing TGFA, with an estimated sensitivity of < 1pg/ml. The ELISA analytical biochemical technique of the MBS175792 kit is based on TGFA antibody-TGFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum, plasma (heparin, EDTA) and human milk. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TGFA elisa kit

   Principle of the assay: human TGFalpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFalpha has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFalpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGFalpha amount of sample captured in plate.
Background: Transforming growth factor alpha (TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain.1Transforming growth factor alpha gene (TGFA) maps to human chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma.2 Synthetic TGF-alpha was as active as murine epidermal growth factor inbinding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.3
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 Typical Testing Data/Standard Curve (for reference only) of TGFA elisa kit    TGFA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175792. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TGFA. It may not necessarily be applicable to this product.
 NCBI GI #    135689
 NCBI GeneID    7039
 NCBI Accession #    P01135.1 [Other Products]
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 UniProt Primary Accession #    P01135 [Other Products]
 UniProt Secondary Accession #    Q15577; Q53SK7; Q9BS56; Q9UEI3; Q9UKM1; Q9UKM2; Q9UKM3; A8K286 [Other Products]
 UniProt Related Accession #    P01135 [Other Products]
 Molecular Weight    17,329 Da
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 NCBI Official Full Name    Protransforming growth factor alpha
 NCBI Official Synonym Full Names    transforming growth factor, alpha
 NCBI Official Symbol    TGFA [Similar Products]
 NCBI Official Synonym Symbols   
TFGA
[Similar Products]
 NCBI Protein Information    protransforming growth factor alpha; TGF-alpha
 UniProt Protein Name    Protransforming growth factor alpha
 UniProt Synonym Protein Names   
EGF-like TGF; ETGF; TGF type 1
 Protein Family    Transforming growth factor
 UniProt Gene Name    TGFA [Similar Products]
 UniProt Synonym Gene Names    TGF-alpha; ETGF [Similar Products]
 UniProt Entry Name    TGFA_HUMAN
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 NCBI Summary for TGFA    This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
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 UniProt Comments for TGFA    TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: extracellular space; cell surface; integral to plasma membrane; perinuclear region of cytoplasm; basolateral plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus

Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding

Biological Process: response to drug; epidermal growth factor receptor signaling pathway; cell proliferation; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; wound healing; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
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 Research Articles on TGFA    1. SPC18 contributes to malignant progression through promotion of TGF-alpha secretion in gastric cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TGFA elisa kitPathways associated with TGFA elisa kit
 Reference Product  PubMed Publications
 EGFR elisa kit  >41 publications with TGFA and EGFR
 TNF elisa kit  >7 publications with TGFA and TNF
 ERBB2 elisa kit  >7 publications with TGFA and ERBB2
 IL6 elisa kit  >5 publications with TGFA and IL6
 ERBB4 elisa kit  >3 publications with TGFA and ERBB4
 TP53 elisa kit  >3 publications with TGFA and TP53
 ERBB3 elisa kit  >2 publications with TGFA and ERBB3
 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 ErbB Receptor Signaling Network Pathway antibodies  ErbB Receptor Signaling Network Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 FOXM1 Transcription Factor Network Pathway antibodies  FOXM1 Transcription Factor Network Pathway Diagram
 Glioma Pathway antibodies  Glioma Pathway Diagram
 Glioma Pathway antibodies  Glioma Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
Diseases associated with TGFA elisa kitOrgans/Tissues associated with TGFA elisa kit
 Disease Name  Pubmed Publications
 Genetic Predisposition to Disease Antibodies  >20 publications with TGFA and Genetic Predisposition to Disease
 Fatty Liver Antibodies  >16 publications with TGFA and Fatty Liver
 Liver Diseases Antibodies  >10 publications with TGFA and Liver Diseases
 Liver Neoplasms Antibodies  >10 publications with TGFA and Liver Neoplasms
 Head and Neck Neoplasms Antibodies  >9 publications with TGFA and Head and Neck Neoplasms
 Breast Neoplasms Antibodies  >9 publications with TGFA and Breast Neoplasms
 Inflammation Antibodies  >7 publications with TGFA and Inflammation
 Nervous System Diseases Antibodies  >7 publications with TGFA and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >7 publications with TGFA and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >7 publications with TGFA and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >40 publications with TGFA and Blood
 Muscle Antibodies  >11 publications with TGFA and Muscle
 Intestine Antibodies  >10 publications with TGFA and Intestine
 Lung Antibodies  >8 publications with TGFA and Lung
 Mouth Antibodies  >7 publications with TGFA and Mouth
 Stomach Antibodies  >7 publications with TGFA and Stomach
 Kidney Antibodies  >7 publications with TGFA and Kidney
 Skin Antibodies  >6 publications with TGFA and Skin
 Nerve Antibodies  >4 publications with TGFA and Nerve
 Thyroid Antibodies  >4 publications with TGFA and Thyroid
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