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TRAP1 elisa kit :: Human TNF receptor-associated protein 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS706000
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TNF receptor-associated protein 1 (TRAP1), ELISA Kit

★Popular Item★
 Also Known As   

Human Heat shock protein 75 kDa, mitochondrial, TRAP1/HSP75 ELISA Kit

 Product Synonym Names    Human Heat shock protein 75 kDa; mitochondrial (TRAP1/HSP75) ELISA kit; HSP75; HSP90L; heat shock protein 75; tumor necrosis factor type 1 receptor associated protein; TNF receptor-associated protein 1
 Product Gene Name   

TRAP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS706000 Testing Data    Testing Data PDF
 OMIM    606219
 3D Structure    ModBase 3D Structure for Q12931
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human TRAP1. No significant cross-reactivity or interference between human TRAP1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.6 25 ng/ml -40 ng/ml.
 Sensitivity    The minimum detectable dose of human TRAP1 is typically less than 0.156 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TRAP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TRAP1 purchase    MBS706000 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TNF receptor-associated protein 1 (TRAP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.6 25 ng/ml -40 ng/ml in biological research samples containing TRAP1, with an estimated sensitivity of The minimum detectable dose of human TRAP1 is typically less than 0.156 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS706000 kit is based on TRAP1 antibody-TRAP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRAP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRAP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TRAP1 - heat shock protein 75 - is a mitochondrial localized homolog of HSP 90. Publications have shown this protein interacts with tumor necrosis factor like receptor and the transcription factor Rb activating inflammatory pathways. Quantitation of cell samples or large sample arrays are made simple with a TRAP 1 ELISA assay. Available in pre-coated multi wells (48) or micrometer plate kits, these ELISA kits show excellent sensitivity and dynamic range for detection of human HSP 75 kDa. All required reagents are provided in this product, including primary antibody, secondary antibodies, wash products and proteins for standard curve generation. Because of high homology within the TNF receptor factor family, such as TNF receptor protein, soluble TNF receptor and TNF receptor II, any assay kit for mitochondrial TRAP1 requires the high level of specificity and lack of cross reaction available in this TRAP1 ELISA kit.
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Related Product Information for TRAP1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TRAP1 elisa kit    TRAP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS706000. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TRAP1. It may not necessarily be applicable to this product.
 NCBI GI #    440309857
 NCBI GeneID    10131
 NCBI Accession #    NP_001258978.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001272049.1 [Other Products]
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 UniProt Primary Accession #    Q12931 [Other Products]
 UniProt Secondary Accession #    O43642; O75235; Q9UHL5; B4DR68; D3DUC8; F5H897 [Other Products]
 UniProt Related Accession #    Q12931 [Other Products]
 Molecular Weight    80,110 Da
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 NCBI Official Full Name    heat shock protein 75 kDa, mitochondrial isoform 2
 NCBI Official Synonym Full Names    TNF receptor-associated protein 1
 NCBI Official Symbol    TRAP1 [Similar Products]
 NCBI Official Synonym Symbols   
HSP75; HSP 75; HSP90L; TRAP-1
[Similar Products]
 NCBI Protein Information    heat shock protein 75 kDa, mitochondrial; TNFR-associated protein 1; tumor necrosis factor type 1 receptor-associated protein
 UniProt Protein Name    Heat shock protein 75 kDa, mitochondrial
 UniProt Synonym Protein Names   
TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1
 Protein Family    TNF receptor-associated protein
 UniProt Gene Name    TRAP1 [Similar Products]
 UniProt Synonym Gene Names    HSP75; HSP 75; TRAP-1 [Similar Products]
 UniProt Entry Name    TRAP1_HUMAN
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 NCBI Summary for TRAP1    This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
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 UniProt Comments for TRAP1    Function: Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.3 PublicationsManual assertion based on experiment in:Ref.12
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 Research Articles on TRAP1    1. study shows that TRAP1 was overexpressed in most patients with ESCC, and caused an increase in antiapoptosis potency
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TRAP1 elisa kitPathways associated with TRAP1 elisa kit
 Reference Product  PubMed Publications
 PINK1 elisa kit  >9 publications with TRAP1 and PINK1
 Products by Pathway  Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
Diseases associated with TRAP1 elisa kitOrgans/Tissues associated with TRAP1 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >47 publications with TRAP1 and Necrosis
 Carcinoma Antibodies  >16 publications with TRAP1 and Carcinoma
 Cardiovascular Diseases Antibodies  >7 publications with TRAP1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >6 publications with TRAP1 and Disease Models, Animal
 Disease Progression Antibodies  >6 publications with TRAP1 and Disease Progression
 Adenocarcinoma Antibodies  >5 publications with TRAP1 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >5 publications with TRAP1 and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >4 publications with TRAP1 and Neoplasms, Experimental
 Inflammation Antibodies  >3 publications with TRAP1 and Inflammation
 Liver Neoplasms, Experimental Antibodies  >2 publications with TRAP1 and Liver Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >15 publications with TRAP1 and Blood
 Vascular Antibodies  >7 publications with TRAP1 and Vascular
 Muscle Antibodies  >7 publications with TRAP1 and Muscle
 Lung Antibodies  >5 publications with TRAP1 and Lung
 Heart Antibodies  >5 publications with TRAP1 and Heart
 Brain Antibodies  >5 publications with TRAP1 and Brain
 Prostate Antibodies  >3 publications with TRAP1 and Prostate
 Kidney Antibodies  >3 publications with TRAP1 and Kidney
 Liver Antibodies  >3 publications with TRAP1 and Liver
 Embryonic Tissue Antibodies  >2 publications with TRAP1 and Embryonic Tissue
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