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TNNT1 elisa kit :: Human TNNT1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2513112
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   


★Popular Item★
 Also Known As   

Human TNNT1 (Troponin T Type 1, Slow Skeletal) ELISA Kit

 Product Gene Name   

TNNT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    191041
 3D Structure    ModBase 3D Structure for P13805
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human TNNT1. No significant cross-reactivity or interference between Human TNNT1 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
 Intended Uses    This ELISA kit applies to the in vitro quantitative determination of Human TNNT1 concentrations in serum, plasma and other biological fluids.
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNNT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNNT1 purchase    MBS2513112 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TNNT1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing TNNT1, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2513112 kit is based on TNNT1 antibody-TNNT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNNT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNNT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNNT1 elisa kit

   Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNNT1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNNT1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNNT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TNNT1. You can calculate the concentration of TNNT1 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TNNT1 elisa kit    TNNT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2513112. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNNT1. It may not necessarily be applicable to this product.
 NCBI GI #    187173288
 NCBI GeneID    7138
 NCBI Accession #    NP_003274.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_003283.5 [Other Products]
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 UniProt Primary Accession #    P13805 [Other Products]
 UniProt Secondary Accession #    O95472; Q16061; Q5U0E1 [Other Products]
 UniProt Related Accession #    P13805 [Other Products]
 Molecular Weight    31,242 Da
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 NCBI Official Full Name    troponin T, slow skeletal muscle isoform a
 NCBI Official Synonym Full Names    troponin T type 1 (skeletal, slow)
 NCBI Official Symbol    TNNT1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    troponin T, slow skeletal muscle; slow skeletal muscle troponin T; troponin-T1, skeletal, slow
 UniProt Protein Name    Troponin T, slow skeletal muscle
 UniProt Synonym Protein Names   
Slow skeletal muscle troponin T; sTnT
 Protein Family    Troponin
 UniProt Gene Name    TNNT1 [Similar Products]
 UniProt Synonym Gene Names    TNT; TnTs; sTnT [Similar Products]
 UniProt Entry Name    TNNT1_HUMAN
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 NCBI Summary for TNNT1    This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TNNT1    TNNT1: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT1 are the cause of nemaline myopathy type 5 (NEM5); also known as Amish nemaline myopathy (ANM). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 5 is a severe and progressive form common among Old Order Amish. Affected infants display tremors with hypotonia and mild contractures of the shoulders and hips. Proximal contractures progressively weaken and a pectus carinatum deformity develops before children die of respiratory insufficiency, usually in the second year. Belongs to the troponin T family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: troponin complex; cytosol

Molecular Function: troponin T binding; tropomyosin binding

Biological Process: skeletal muscle contraction; slow-twitch skeletal muscle fiber contraction; negative regulation of muscle contraction; muscle filament sliding

Disease: Nemaline Myopathy 5
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 Research Articles on TNNT1    1. Troponin T1 blood levels had a positive association with increased risk for hypertrophic cardiomyopathy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNNT1 elisa kitPathways associated with TNNT1 elisa kit
 Reference Product  PubMed Publications
 NEB elisa kit  >10 publications with TNNT1 and NEB
 TPM2 elisa kit  >10 publications with TNNT1 and TPM2
 TNNI2 elisa kit  >3 publications with TNNT1 and TNNI2
 TNNI3 elisa kit  >2 publications with TNNT1 and TNNI3
 TNNC1 elisa kit  >1 publications with TNNT1 and TNNC1
 TNNI1 elisa kit  >1 publications with TNNT1 and TNNI1
 TPM1 elisa kit  >1 publications with TNNT1 and TPM1
 Products by Pathway  Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
Diseases associated with TNNT1 elisa kitOrgans/Tissues associated with TNNT1 elisa kit
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >3 publications with TNNT1 and Brain Diseases
 Disease Models, Animal Antibodies  >2 publications with TNNT1 and Disease Models, Animal
 Neurotoxicity Syndromes Antibodies  >1 publications with TNNT1 and Neurotoxicity Syndromes
 Neoplasm Metastasis Antibodies  >1 publications with TNNT1 and Neoplasm Metastasis
 Weight Loss Antibodies  >1 publications with TNNT1 and Weight Loss
 Inflammation Antibodies  >1 publications with TNNT1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >25 publications with TNNT1 and Muscle
 Heart Antibodies  >5 publications with TNNT1 and Heart
 Brain Antibodies  >4 publications with TNNT1 and Brain
 Lung Antibodies  >2 publications with TNNT1 and Lung
 Bone Antibodies  >1 publications with TNNT1 and Bone
 Uncharacterized Tissue Antibodies  >1 publications with TNNT1 and Uncharacterized Tissue
 Nerve Antibodies  >1 publications with TNNT1 and Nerve
 Embryonic Tissue Antibodies  >1 publications with TNNT1 and Embryonic Tissue
 Eye Antibodies  >1 publications with TNNT1 and Eye
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