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TNC elisa kit :: Human Tenascin-C/TNC PicoKine ELISA Kit

Scan QR to view Datasheet Catalog #    MBS177649
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1x96 Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Tenascin-C/TNC PicoKine, ELISA Kit

 Also Known As   

Human Tenascin-C/TNC PicoKine ELISA Kit

 Product Synonym Names    Tenascin; 150 225; Cytotactin; Glioma associated extracellular matrix antigen; Glioma-associated-extracellular matrix antigen; GMEM; GP 150 225; GP 150-225 GP; Hexabrachion; HXB; JI; MGC167029; Miotendinous antigen; Myotendinous antigen; Neuronectin; TENA_HUMAN; Tenascin; Tenascin-C; Tenascin-C isoform 14/AD1/16; TenascinC; TN; TN C; TN-C; TNC ; tenascin C
 Product Gene Name   

TNC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    187380
 3D Structure    ModBase 3D Structure for P24821
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 Species Reactivity    Human
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 Specificity    Natural and recombinant human Tenascin-C
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 Samples    Cell culture supernates, serum, plasma (heparin, EDTA) and urine
 Assay Type    Quantitative Sandwich
 Detection Range    93.7pg/ml-6000pg/ml
 Sensitivity    <10pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of TNC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNC purchase    MBS177649 is a ready-to-use microwell, strip-or-full plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tenascin-C/TNC PicoKine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 93.7pg/ml-6000pg/ml in biological research samples containing TNC, with an estimated sensitivity of <10pg/ml. The ELISA analytical biochemical technique of the MBS177649 kit is based on TNC antibody-TNC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum, plasma (heparin, EDTA) and urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TNC elisa kit

   Principle of the Assay: The human Tenascin-C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tenascin-C has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: G23-P625) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tenascin-C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution The density of yellow is proportional to the human Tenascin-C amount of sample captured in plate.

Background: Tenascln C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.
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 Typical Testing Data/Standard Curve (for reference only) of TNC elisa kit    TNC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS177649
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NCBI/Uniprot data below describe general gene information for TNC. It may not necessarily be applicable to this product.
 NCBI GI #    153946395
 NCBI GeneID    3371
 NCBI Accession #    NP_002151.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002160.3 [Other Products]
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 UniProt Primary Accession #    P24821 [Other Products]
 UniProt Secondary Accession #    Q14583; Q15567; Q5T7S3; C9IYT7; C9J575; C9J6D9; C9J848 [Other Products]
 UniProt Related Accession #    P24821 [Other Products]
 Molecular Weight    171,360 Da [Similar Products]
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 NCBI Official Full Name    tenascin
 NCBI Official Synonym Full Names    tenascin C
 NCBI Official Symbol    TNC [Similar Products]
 NCBI Official Synonym Symbols   
GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225
[Similar Products]
 NCBI Protein Information    tenascin
 UniProt Protein Name    Tenascin
 UniProt Synonym Protein Names   
Cytotactin; GMEM; GP 150-225; Glioma-associated-extracellular matrix antigen; Hexabrachion; JI; Myotendinous antigen; Neuronectin; Tenascin-C; TN-C
 Protein Family    Tenascin
 UniProt Gene Name    TNC [Similar Products]
 UniProt Synonym Gene Names    HXB; TN; TN-C [Similar Products]
 UniProt Entry Name    TENA_HUMAN
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 NCBI Summary for TNC    This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
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 UniProt Comments for TNC    TNC: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. Belongs to the tenascin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: basement membrane; extracellular matrix; extracellular region; extracellular space; focal adhesion; membrane

Molecular Function: syndecan binding

Biological Process: axon regeneration in the peripheral nervous system; cell adhesion; extracellular matrix organization and biogenesis; negative regulation of cell adhesion; neuromuscular junction development; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of cell proliferation; response to ethanol; response to mechanical stimulus; response to wounding; wound healing

Disease: Deafness, Autosomal Dominant 56
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 Research Articles on TNC    1. Serum TNC levels are significantly raised and correlate with various clinical and laboratory variables of disease activity in children with enthesitis-related arthritis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNC elisa kitPathways associated with TNC elisa kit
 Reference Product  PubMed Publications
 EGFR elisa kit  >14 publications with TNC and EGFR
 RHOA elisa kit  >9 publications with TNC and RHOA
 MMP3 elisa kit  >9 publications with TNC and MMP3
 FGF2 elisa kit  >7 publications with TNC and FGF2
 PAX6 elisa kit  >2 publications with TNC and PAX6
 NPSR1 elisa kit  >1 publications with TNC and NPSR1
 CNTN1 elisa kit  >1 publications with TNC and CNTN1
 PTK2 elisa kit  >1 publications with TNC and PTK2
 SDC4 elisa kit  >1 publications with TNC and SDC4
 Products by Pathway  Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
Diseases associated with TNC elisa kitOrgans/Tissues associated with TNC elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >109 publications with TNC and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >97 publications with TNC and Nervous System Diseases
 Heart Diseases Antibodies  >77 publications with TNC and Heart Diseases
 Disease Models, Animal Antibodies  >59 publications with TNC and Disease Models, Animal
 Brain Diseases Antibodies  >56 publications with TNC and Brain Diseases
 Inflammation Antibodies  >52 publications with TNC and Inflammation
 Lung Diseases Antibodies  >30 publications with TNC and Lung Diseases
 Necrosis Antibodies  >27 publications with TNC and Necrosis
 Breast Neoplasms Antibodies  >24 publications with TNC and Breast Neoplasms
 Fibrosis Antibodies  >23 publications with TNC and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >621 publications with TNC and Muscle
 Heart Antibodies  >247 publications with TNC and Heart
 Brain Antibodies  >139 publications with TNC and Brain
 Bone Antibodies  >133 publications with TNC and Bone
 Bone Marrow Antibodies  >102 publications with TNC and Bone Marrow
 Lung Antibodies  >51 publications with TNC and Lung
 Embryonic Tissue Antibodies  >50 publications with TNC and Embryonic Tissue
 Skin Antibodies  >26 publications with TNC and Skin
 Liver Antibodies  >24 publications with TNC and Liver
 Connective Tissue Antibodies  >24 publications with TNC and Connective Tissue
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