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TK1 elisa kit :: Human Thymidine Kinase 1, Soluble (TK1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021703 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
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 Product Name   

Thymidine Kinase 1, Soluble (TK1), ELISA Kit

★Popular Item★
 Also Known As   

Thymidine Kinase 1, Soluble (TK1) ELISA Kit

 Product Synonym Names    TK2; Thymidine kinase, cytosolic
 Product Gene Name   

TK1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    188300
 3D Structure    ModBase 3D Structure for P04183
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Thymidine Kinase 1, Soluble (TK1).
No significant cross-reactivity or interference between Thymidine Kinase 1, Soluble (TK1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    78-5,000pg/mL
 Sensitivity    < 29pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TK1 purchase    MBS2021703 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thymidine Kinase 1, Soluble (TK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5,000pg/mL in biological research samples containing TK1, with an estimated sensitivity of < 29pg/mL. The ELISA analytical biochemical technique of the MBS2021703 kit is based on TK1 antibody-TK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TK1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidine Kinase 1, Soluble (TK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidine Kinase 1, Soluble (TK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidine Kinase 1, Soluble (TK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymidine Kinase 1, Soluble (TK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for TK1 elisa kit    Enzyme & Kinase
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 Typical Testing Data/Standard Curve (for reference only) of TK1 elisa kit    TK1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021703. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TK1. It may not necessarily be applicable to this product.
 NCBI GI #    52632392
 NCBI GeneID    7083
 NCBI Accession #    AAH06484.1 [Other Products]
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 UniProt Primary Accession #    P04183 [Other Products]
 UniProt Secondary Accession #    Q969V0; Q9UMG9; B2RC58 [Other Products]
 UniProt Related Accession #    P04183 [Other Products]
 Molecular Weight    25,469 Da
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 NCBI Official Full Name    Thymidine kinase 1, soluble
 NCBI Official Synonym Full Names    thymidine kinase 1, soluble
 NCBI Official Symbol    TK1 [Similar Products]
 NCBI Official Synonym Symbols   
TK2
[Similar Products]
 NCBI Protein Information    thymidine kinase, cytosolic
 UniProt Protein Name    Thymidine kinase, cytosolic
 Protein Family    Thymidine kinase
 UniProt Gene Name    TK1 [Similar Products]
 UniProt Entry Name    KITH_HUMAN
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 UniProt Comments for TK1    TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Protein type: EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Kinase, other; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 17q23.2-q25.3

Cellular Component: cytosol

Molecular Function: identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding

Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; deoxyribonucleoside monophosphate biosynthetic process; protein homotetramerization; fetal process involved in parturition; response to cortisol stimulus; pyrimidine base metabolic process; gut development; response to copper ion; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine nucleoside salvage; thymidine metabolic process; phosphorylation
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 Research Articles on TK1    1. A regression analysis showed that only TK1 levels were significant (relative risk (RR)=1.03 for each unit, confidence interval (CI)=1-1.05; p=0.015) for diagnosing a true transformation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TK1 elisa kitPathways associated with TK1 elisa kit
 Reference Product  PubMed Publications
 TYMS elisa kit  >5 publications with TK1 and TYMS
 RRM2 elisa kit  >3 publications with TK1 and RRM2
 DCTD elisa kit  >2 publications with TK1 and DCTD
 CDK1 elisa kit  >2 publications with TK1 and CDK1
 CDC20 elisa kit  >2 publications with TK1 and CDC20
 E2F1 elisa kit  >2 publications with TK1 and E2F1
 UBE2C elisa kit  >1 publications with TK1 and UBE2C
 BIRC5 elisa kit  >1 publications with TK1 and BIRC5
 TYMP elisa kit  >1 publications with TK1 and TYMP
 Products by Pathway  Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 Fluoropyrimidine Activity Pathway antibodies  Fluoropyrimidine Activity Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
Diseases associated with TK1 elisa kitOrgans/Tissues associated with TK1 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >48 publications with TK1 and Carcinoma
 Breast Neoplasms Antibodies  >30 publications with TK1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >26 publications with TK1 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >23 publications with TK1 and Lung Neoplasms
 Lung Diseases Antibodies  >20 publications with TK1 and Lung Diseases
 Adenocarcinoma Antibodies  >19 publications with TK1 and Adenocarcinoma
 Disease Models, Animal Antibodies  >15 publications with TK1 and Disease Models, Animal
 Colorectal Neoplasms Antibodies  >14 publications with TK1 and Colorectal Neoplasms
 Brain Diseases Antibodies  >10 publications with TK1 and Brain Diseases
 Nervous System Diseases Antibodies  >9 publications with TK1 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >101 publications with TK1 and Blood
 Lung Antibodies  >33 publications with TK1 and Lung
 Brain Antibodies  >19 publications with TK1 and Brain
 Liver Antibodies  >18 publications with TK1 and Liver
 Lymph Antibodies  >16 publications with TK1 and Lymph
 Lymph Node Antibodies  >16 publications with TK1 and Lymph Node
 Bone Antibodies  >12 publications with TK1 and Bone
 Muscle Antibodies  >10 publications with TK1 and Muscle
 Bone Marrow Antibodies  >8 publications with TK1 and Bone Marrow
 Kidney Antibodies  >7 publications with TK1 and Kidney
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