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ADPN elisa kit :: Human Total Adiponectin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS720699
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $470 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $470 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $675 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,850 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,850 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,340 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,340 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Total Adiponectin (ADPN), ELISA Kit

 Also Known As   

Human Total Adiponectin ELISA Kit

 Product Gene Name   

ADPN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sandwich ELISA Manual Insert    Sandwich ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ADP. No significant cross-reactivity or interference between ADP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADP and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich (Quantitative)
 Sensitivity    0.1mug/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADPN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADPN purchase    MBS720699 is a ready-to-use microwell, strip plate Competitive or Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Total Adiponectin (ADPN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADPN, with an estimated sensitivity of 0.1mug/mL. The ELISA analytical biochemical technique of the MBS720699 kit is based on ADPN antibody-ADPN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADPN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADPN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ADPN elisa kit

   Intended Uses: This ADP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ADP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADP. Standards or samples are then added to the microtiter plate wells and ADP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADP are added to each well to "sandwich" the ADP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADP concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ADPN elisa kit    ADPN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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