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TFAM elisa kit :: Human Transcription Factor A, Mitochondrial (TFAM) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020891 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $380 +1 FREE 8GB USB
48-Strip-Wells  /  $565 +1 FREE 8GB USB
96-Strip-Wells  /  $765 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,130 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,670 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Transcription Factor A, Mitochondrial (TFAM), ELISA Kit

★Popular Item★
 Also Known As   

Transcription Factor A, Mitochondrial (TFAM) ELISA Kit

 Product Synonym Names    MtTF1; TCF6; TCF6L2; mtTFA; Mitochondrial transcription factor 1; Transcription factor 6-like 2
 Product Gene Name   

TFAM elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600438
 3D Structure    ModBase 3D Structure for Q00059
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of TFAM. No significant cross-reactivity or interference between TFAM and analogues was observed.
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 Samples    Human tissue homogenates, cell lysates and other biological fluids.
 Assay Type    Sandwich (Quantitative)
 Detection Range    0.312-20ng/mL.
 Sensitivity    0.121ng/mL.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TFAM were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TFAM were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TFAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TFAM purchase    MBS2020891 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transcription Factor A, Mitochondrial (TFAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing TFAM, with an estimated sensitivity of 0.121ng/mL. The ELISA analytical biochemical technique of the MBS2020891 kit is based on TFAM antibody-TFAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TFAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TFAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TFAM elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TFAM in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TFAM. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TFAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TFAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TFAM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TFAM elisa kit    TFAM elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020891. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TFAM. It may not necessarily be applicable to this product.
 NCBI GI #    4507401
 NCBI GeneID    7019
 NCBI Accession #    NP_003192.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003201.2 [Other Products]
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 UniProt Primary Accession #    Q00059 [Other Products]
 UniProt Secondary Accession #    Q5U0C6; A8MRB2; A9QXC6; B5BU05 [Other Products]
 UniProt Related Accession #    Q00059 [Other Products]
 Molecular Weight    25,466 Da
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 NCBI Official Full Name    transcription factor A, mitochondrial isoform 1
 NCBI Official Synonym Full Names    transcription factor A, mitochondrial
 NCBI Official Symbol    TFAM [Similar Products]
 NCBI Official Synonym Symbols   
TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
[Similar Products]
 NCBI Protein Information    transcription factor A, mitochondrial
 UniProt Protein Name    Transcription factor A, mitochondrial
 UniProt Synonym Protein Names   
Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2
 Protein Family    Transcription factor
 UniProt Gene Name    TFAM [Similar Products]
 UniProt Synonym Gene Names    TCF6; TCF6L2; mtTFA; MtTF1; TCF-6 [Similar Products]
 UniProt Entry Name    TFAM_HUMAN
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 NCBI Summary for TFAM    This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
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 UniProt Comments for TFAM    TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.

Protein type: DNA-binding; Transcription regulation

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: mitochondrion; mitochondrial matrix; nucleus; cytosol

Molecular Function: protein binding; heat shock protein binding; chromatin binding; DNA bending activity; transcription factor activity

Biological Process: chromatin remodeling; mitochondrial respiratory chain complex assembly; mitochondrion organization and biogenesis; regulation of transcription from RNA polymerase I promoter; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; gene expression; DNA-dependent DNA replication; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter
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 Research Articles on TFAM    1. expression of PGC1alpha and TFAM varies between ovarian carcinoma subtypes; clear-cell carcinoma consists of undetectability of PGC1alpha/TFAM, and low ERalpha/Ki-67. high-grade serous carcinomas had a converse state of PGC1alpha/TFAM, ERalpha positivity and high Ki-67 index
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TFAM elisa kitPathways associated with TFAM elisa kit
 Reference Product  PubMed Publications
 NRF1 elisa kit  >107 publications with TFAM and NRF1
 TFB2M elisa kit  >41 publications with TFAM and TFB2M
 TFB1M elisa kit  >26 publications with TFAM and TFB1M
 POLRMT elisa kit  >18 publications with TFAM and POLRMT
 GABPA elisa kit  >12 publications with TFAM and GABPA
 GABPB1 elisa kit  >1 publications with TFAM and GABPB1
 TXN2 elisa kit  >1 publications with TFAM and TXN2
 Products by Pathway  Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Mitochondrial Gene Expression Pathway antibodies  Mitochondrial Gene Expression Pathway Diagram
 Mitochondrial Biogenesis Pathway antibodies  Mitochondrial Biogenesis Pathway Diagram
 Mitochondrial Transcription Initiation Pathway antibodies  Mitochondrial Transcription Initiation Pathway Diagram
 Organelle Biogenesis And Maintenance Pathway antibodies  Organelle Biogenesis And Maintenance Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 Transcription From Mitochondrial Promoters Pathway antibodies  Transcription From Mitochondrial Promoters Pathway Diagram
Diseases associated with TFAM elisa kitOrgans/Tissues associated with TFAM elisa kit
 Disease Name  Pubmed Publications
 Mitochondrial Diseases Antibodies  >101 publications with TFAM and Mitochondrial Diseases
 Nervous System Diseases Antibodies  >87 publications with TFAM and Nervous System Diseases
 Disease Models, Animal Antibodies  >54 publications with TFAM and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >49 publications with TFAM and Cardiovascular Diseases
 Insulin Resistance Antibodies  >41 publications with TFAM and Insulin Resistance
 Heart Diseases Antibodies  >34 publications with TFAM and Heart Diseases
 Inflammation Antibodies  >32 publications with TFAM and Inflammation
 Necrosis Antibodies  >27 publications with TFAM and Necrosis
 Liver Diseases Antibodies  >19 publications with TFAM and Liver Diseases
 Hyperglycemia Antibodies  >17 publications with TFAM and Hyperglycemia
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >204 publications with TFAM and Muscle
 Heart Antibodies  >86 publications with TFAM and Heart
 Blood Antibodies  >78 publications with TFAM and Blood
 Brain Antibodies  >77 publications with TFAM and Brain
 Liver Antibodies  >75 publications with TFAM and Liver
 Connective Tissue Antibodies  >31 publications with TFAM and Connective Tissue
 Vascular Antibodies  >22 publications with TFAM and Vascular
 Lung Antibodies  >18 publications with TFAM and Lung
 Kidney Antibodies  >15 publications with TFAM and Kidney
 Thyroid Antibodies  >15 publications with TFAM and Thyroid
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