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GLI3 elisa kit :: Human Transcriptional activator GLI3 (GLI3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7250877
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
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 Product Name   

Transcriptional activator GLI3 (GLI3), ELISA Kit

★Popular Item★
 Also Known As   

Human Transcriptional activator GLI3 (GLI3) ELISA Kit

 Product Gene Name   

GLI3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    175700
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of GLI3. No significant cross-reactivity or interference between GLI3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLI3 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GLI3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GLI3 purchase    MBS7250877 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transcriptional activator GLI3 (GLI3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing GLI3, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7250877 kit is based on GLI3 antibody-GLI3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLI3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLI3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GLI3 elisa kit

   Intended Uses: This GLI3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of human GLI3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||GLI3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GLI3 antibody and an GLI3-HRP conjugate. The assay sample and buffer are incubated together with GLI3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLI3 concentration since GLI3 from samples and GLI3-HRP conjugate compete for the anti-GLI3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GLI3 from the sample, fewer sites are left to bind GLI3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLI3 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for GLI3 elisa kit    Epigenetics and Nuclear Signaling
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 Typical Testing Data/Standard Curve of GLI3 elisa kit    GLI3 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7250877. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GLI3. It may not necessarily be applicable to this product.
 NCBI GI #    119393899
 NCBI GeneID    2737
 NCBI Accession #    NP_000159.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000168.5 [Other Products]
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 UniProt Secondary Accession #    O75219; Q17RW4; Q75MT0; Q75MU9; Q9UDT5; Q9UJ39; A4D1W1 [Other Products]
 UniProt Related Accession #    P10071 [Other Products]
 Molecular Weight    169,863 Da
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 NCBI Official Full Name    transcriptional activator GLI3
 NCBI Official Synonym Full Names    GLI family zinc finger 3
 NCBI Official Symbol    GLI3 [Similar Products]
 NCBI Official Synonym Symbols   
PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190
[Similar Products]
 NCBI Protein Information    transcriptional activator GLI3; oncogene GLI3; zinc finger protein GLI3; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3
 UniProt Protein Name    Transcriptional activator GLI3
 UniProt Synonym Protein Names   
GLI3 form of 190 kDa; GLI3-190; GLI3 full length protein; GLI3FL
 Protein Family    Transcriptional activator
 UniProt Gene Name    GLI3 [Similar Products]
 UniProt Synonym Gene Names    GLI3-190; GLI3FL; GLI3-83 [Similar Products]
 UniProt Entry Name    GLI3_HUMAN
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 NCBI Summary for GLI3    This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GLI3    GLI3: Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. Defects in GLI3 are the cause of Greig cephalo-poly- syndactyly syndrome (GCPS). GCPS is an autosomal dominant disorder affecting limb and craniofacial development. It is characterized by pre- and postaxial polydactyly, syndactyly of fingers and toes, macrocephaly and hypertelorism. Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). PHS is characterized by a wide range of clinical manifestations. It mainly associates central or postaxial polydactyly, syndactyly, and hypothalamic hamartoma. Malformations are frequent in the viscera, e.g. anal atresia, bifid uvula, congenital heart malformations, pulmonary or renal dysplasia. It is an autosomal dominant disorder. Defects in GLI3 are a cause of polydactyly postaxial type A1 (PAPA1). A trait characterized by an extra digit in the ulnar and/or fibular side of the upper and/or lower extremities. The extra digit is well formed and articulates with the fifth, or extra, metacarpal/metatarsal, and thus it is usually functional. Defects in GLI3 are a cause of polydactyly postaxial type B polydactyly (PAPB). A trait characterized by an extra digit in the ulnar and/or fibular side of the upper and/or lower extremities. The extra digit is not well formed and is frequently in the form of a skin. Defects in GLI3 are a cause of polydactyly preaxial type 4 (POP4). Polydactyly preaxial type 4 (i.e. polydactyly on the radial/tibial side of the hand/foot) covers a heterogeneous group of entities. In preaxial polydactyly type IV, the thumb shows only the mildest degree of duplication, and syndactyly of various degrees affects fingers 3 and 4. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: nucleoplasm; transcriptional repressor complex; cytoplasm; nuclear speck; Srb-mediator complex; cytosol; nucleus; cilium

Molecular Function: histone acetyltransferase binding; protein binding; histone deacetylase binding; metal ion binding; beta-catenin binding; chromatin binding; transcription factor activity

Biological Process: developmental growth; radial glial cell differentiation in the forebrain; heart development; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; anterior/posterior pattern formation; embryonic digestive tract morphogenesis; oligodendrocyte differentiation; lateral ganglionic eminence cell proliferation; embryonic gut development; positive regulation of neuroblast proliferation; transcription, DNA-dependent; optic nerve morphogenesis; negative thymic T cell selection; negative regulation of neuron differentiation; positive regulation of chondrocyte differentiation; camera-type eye morphogenesis; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; metanephros development; smoothened signaling pathway involved in spinal cord motor neuron cell fate specification; negative regulation of apoptosis; tongue development; axon guidance; wound healing; forebrain dorsal/ventral pattern formation; negative regulation of transcription from RNA polymerase II promoter; palate development; nose morphogenesis; negative regulation of cell proliferation; melanocyte differentiation; smoothened signaling pathway involved in ventral spinal cord interneuron specification; proximal/distal pattern formation; smoothened signaling pathway; in utero embryonic development; hippocampus development; limb morphogenesis; embryonic skeletal morphogenesis; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; positive regulation of protein import into nucleus; cerebral cortex radial glia guided migration; ureteric bud branching; negative regulation of smoothened signaling pathway; protein processing; hindgut morphogenesis; positive regulation of alpha-beta T cell differentiation; negative regulation of alpha-beta T cell differentiation; lung development

Disease: Polydactyly, Postaxial, Type A1; Hypothalamic Hamartomas; Polydactyly, Preaxial Iv; Pallister-hall Syndrome; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Greig Cephalopolysyndactyly Syndrome
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 Research Articles on GLI3    1. The findings of this study indicated that neuronal differentiation is associated with Gli3 expression in medulloblastoma cells, and that this feature predicts a favorable outcome for patients with medulloblastoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GLI3 elisa kitPathways associated with GLI3 elisa kit
 Reference Product  PubMed Publications
 SHH elisa kit  >295 publications with GLI3 and SHH
 SMO elisa kit  >66 publications with GLI3 and SMO
 SUFU elisa kit  >29 publications with GLI3 and SUFU
 FGF10 elisa kit  >9 publications with GLI3 and FGF10
 RAB23 elisa kit  >6 publications with GLI3 and RAB23
 SPOP elisa kit  >4 publications with GLI3 and SPOP
 IFT172 elisa kit  >3 publications with GLI3 and IFT172
 STK36 elisa kit  >1 publications with GLI3 and STK36
 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Events Mediated By Gli Proteins Pathway antibodies  Hedgehog Signaling Events Mediated By Gli Proteins Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 CAMP Signaling Pathway antibodies  CAMP Signaling Pathway Diagram
 CAMP Signaling Pathway antibodies  CAMP Signaling Pathway Diagram
Diseases associated with GLI3 elisa kitOrgans/Tissues associated with GLI3 elisa kit
 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >187 publications with GLI3 and Congenital Abnormalities
 Polydactyly Antibodies  >129 publications with GLI3 and Polydactyly
 Nervous System Diseases Antibodies  >75 publications with GLI3 and Nervous System Diseases
 Greig cephalopolysyndactyly syndrome Antibodies  >53 publications with GLI3 and Greig cephalopolysyndactyly syndrome
 Pallister-Hall Syndrome Antibodies  >52 publications with GLI3 and Pallister-Hall Syndrome
 Neural Tube Defects Antibodies  >28 publications with GLI3 and Neural Tube Defects
 Chromosome Aberrations Antibodies  >24 publications with GLI3 and Chromosome Aberrations
 Disease Models, Animal Antibodies  >24 publications with GLI3 and Disease Models, Animal
 Polydactyly, preaxial 4 Antibodies  >12 publications with GLI3 and Polydactyly, preaxial 4
 Adenocarcinoma Antibodies  >11 publications with GLI3 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >204 publications with GLI3 and Embryonic Tissue
 Brain Antibodies  >113 publications with GLI3 and Brain
 Bone Antibodies  >105 publications with GLI3 and Bone
 Eye Antibodies  >33 publications with GLI3 and Eye
 Muscle Antibodies  >25 publications with GLI3 and Muscle
 Lung Antibodies  >25 publications with GLI3 and Lung
 Connective Tissue Antibodies  >25 publications with GLI3 and Connective Tissue
 Skin Antibodies  >23 publications with GLI3 and Skin
 Heart Antibodies  >19 publications with GLI3 and Heart
 Blood Antibodies  >19 publications with GLI3 and Blood
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