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TRAM1 elisa kit :: Human Translocation Associated Membrane Protein 1 (TRAM1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024152 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Translocation Associated Membrane Protein 1 (TRAM1), ELISA Kit

 Also Known As   

Translocation Associated Membrane Protein 1 (TRAM1) ELISA Kit

 Product Synonym Names    TRAM; TRAMP; Translocating chain-associated membrane protein 1
 Product Gene Name   

TRAM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605190
 3D Structure    ModBase 3D Structure for Q15629
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Translocation Associated Membrane Protein 1 (TRAM1).
No significant cross-reactivity or interference between Translocation Associated Membrane Protein 1 (TRAM1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.059ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Translocation Associated Membrane Protein 1 (TRAM1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Translocation Associated Membrane Protein 1 (TRAM1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TRAM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TRAM1 purchase    MBS2024152 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Translocation Associated Membrane Protein 1 (TRAM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing TRAM1, with an estimated sensitivity of < 0.059ng/mL. The ELISA analytical biochemical technique of the MBS2024152 kit is based on TRAM1 antibody-TRAM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRAM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRAM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TRAM1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocation Associated Membrane Protein 1 (TRAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Translocation Associated Membrane Protein 1 (TRAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocation Associated Membrane Protein 1 (TRAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Translocation Associated Membrane Protein 1 (TRAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for TRAM1 elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of TRAM1 elisa kit    TRAM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024152. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TRAM1. It may not necessarily be applicable to this product.
 NCBI GI #    22902167
 NCBI GeneID    23471
 NCBI Accession #    AAH37738.1 [Other Products]
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 UniProt Primary Accession #    Q15629 [Other Products]
 UniProt Secondary Accession #    B4E0K2 [Other Products]
 UniProt Related Accession #    Q15629 [Other Products]
 Molecular Weight    39,683 Da
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 NCBI Official Full Name    Translocation associated membrane protein 1
 NCBI Official Synonym Full Names    translocation associated membrane protein 1
 NCBI Official Symbol    TRAM1 [Similar Products]
 NCBI Official Synonym Symbols   
TRAM; PNAS8; TRAMP
[Similar Products]
 NCBI Protein Information    translocating chain-associated membrane protein 1
 UniProt Protein Name    Translocating chain-associated membrane protein 1
 Protein Family    Translocating chain-associated membrane protein
 UniProt Gene Name    TRAM1 [Similar Products]
 UniProt Synonym Gene Names    TRAM [Similar Products]
 UniProt Entry Name    TRAM1_HUMAN
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 NCBI Summary for TRAM1    This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq, Oct 2009]
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 UniProt Comments for TRAM1    TRAM1: Stimulatory or required for the translocation of secretory proteins across the ER membrane. Belongs to the TRAM family.

Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; receptor activity

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; viral reproduction; translation; gene expression; cotranslational protein targeting to membrane
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 Research Articles on TRAM1    1. TRAM1 protect HepG2 cells from palmitate-induced insulin resistance. The protection of TRAM1 to HepG2 cells is through endoplasmic reticulum stress-JNK pathway.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TRAM1 elisa kitDiseases associated with TRAM1 elisa kit
 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 SRP-dependent Cotranslational Protein Targeting To Membrane Pathway antibodies  SRP-dependent Cotranslational Protein Targeting To Membrane Pathway Diagram
 Translation Pathway antibodies  Translation Pathway Diagram
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >2 publications with TRAM1 and Liver Diseases
 Breast Neoplasms Antibodies  >2 publications with TRAM1 and Breast Neoplasms
 Fatty Liver Antibodies  >2 publications with TRAM1 and Fatty Liver
 Inflammation Antibodies  >2 publications with TRAM1 and Inflammation
 Necrosis Antibodies  >2 publications with TRAM1 and Necrosis
 Insulin Resistance Antibodies  >1 publications with TRAM1 and Insulin Resistance
 Carcinoma Antibodies  >1 publications with TRAM1 and Carcinoma
 Chromosome Aberrations Antibodies  >1 publications with TRAM1 and Chromosome Aberrations
 Adenocarcinoma Antibodies  >1 publications with TRAM1 and Adenocarcinoma
 Kidney Diseases Antibodies  >1 publications with TRAM1 and Kidney Diseases
Organs/Tissues associated with TRAM1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4 publications with TRAM1 and Liver
 Thyroid Antibodies  >2 publications with TRAM1 and Thyroid
 Kidney Antibodies  >2 publications with TRAM1 and Kidney
 Bone Antibodies  >1 publications with TRAM1 and Bone
 Umbilical Cord Antibodies  >1 publications with TRAM1 and Umbilical Cord
 Mouth Antibodies  >1 publications with TRAM1 and Mouth
 Blood Antibodies  >1 publications with TRAM1 and Blood
 Stomach Antibodies  >1 publications with TRAM1 and Stomach
 Vascular Antibodies  >1 publications with TRAM1 and Vascular
 Prostate Antibodies  >1 publications with TRAM1 and Prostate
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