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TREM2 elisa kit :: Human Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022102 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $380 +1 FREE 8GB USB
48-Strip-Wells  /  $565 +1 FREE 8GB USB
96-Strip-Wells  /  $765 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,130 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,670 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Triggering Receptor Expressed On Myeloid Cells 2 (TREM2), ELISA Kit

 Also Known As   

Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit

 Product Synonym Names    Trem2a; Trem2b; Trem2c; Triggering receptor expressed on monocytes 2
 Product Gene Name   

TREM2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    221770
 3D Structure    ModBase 3D Structure for Q9NZC2
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Triggering Receptor Expressed On Myeloid Cells 2 (TREM2).
No significant cross-reactivity or interference between Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates and other biological fluids
 Assay Type    Sandwich
 Detection Range    62.5-4000pg/mL
 Sensitivity    Typically less than 25.5pg/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of TREM2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TREM2 purchase    MBS2022102 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/mL in biological research samples containing TREM2, with an estimated sensitivity of Typically less than 25.5pg/mL. The ELISA analytical biochemical technique of the MBS2022102 kit is based on TREM2 antibody-TREM2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TREM2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TREM2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TREM2 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triggering Receptor Expressed On Myeloid Cells 2 (TREM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Triggering Receptor Expressed On Myeloid Cells 2 (TREM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Triggering Receptor Expressed On Myeloid Cells 2 (TREM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TREM2 elisa kit    TREM2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022102
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NCBI/Uniprot data below describe general gene information for TREM2. It may not necessarily be applicable to this product.
 NCBI GI #    9507203
 NCBI GeneID    54209
 NCBI Accession #    NP_061838.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_018965.3 [Other Products]
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 UniProt Primary Accession #    Q9NZC2 [Other Products]
 UniProt Secondary Accession #    Q8N5H8; Q8WYN6 [Other Products]
 UniProt Related Accession #    Q9NZC2 [Other Products]
 Molecular Weight    24,669 Da
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 NCBI Official Full Name    triggering receptor expressed on myeloid cells 2 isoform 1
 NCBI Official Synonym Full Names    triggering receptor expressed on myeloid cells 2
 NCBI Official Symbol    TREM2 [Similar Products]
 NCBI Official Synonym Symbols   
TREM-2; Trem2a; Trem2b; Trem2c
[Similar Products]
 NCBI Protein Information    triggering receptor expressed on myeloid cells 2
 UniProt Protein Name    Triggering receptor expressed on myeloid cells 2
 UniProt Synonym Protein Names   
Triggering receptor expressed on monocytes 2
 Protein Family    Triggering receptor expressed on myeloid cells
 UniProt Gene Name    TREM2 [Similar Products]
 UniProt Synonym Gene Names    TREM-2 [Similar Products]
 UniProt Entry Name    TREM2_HUMAN
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 NCBI Summary for TREM2    This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
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 UniProt Comments for TREM2    TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: intracellular membrane-bound organelle; plasma membrane; extracellular region; integral to membrane

Molecular Function: peptidoglycan binding; lipopolysaccharide binding; receptor activity

Biological Process: axon guidance; positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; detection of peptidoglycan; innate immune response; positive regulation of calcium-mediated signaling; humoral immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy
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 Research Articles on TREM2    1. The results of this study suggested that the p.R47H is a risk factor for AD, but not frontotemporal dementia or prion disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TREM2 elisa kitPathways associated with TREM2 elisa kit
 Reference Product  PubMed Publications
 TYROBP elisa kit  >49 publications with TREM2 and TYROBP
 SYK elisa kit  >8 publications with TREM2 and SYK
 PLXNA1 elisa kit  >2 publications with TREM2 and PLXNA1
 VAV3 elisa kit  >1 publications with TREM2 and VAV3
 SEMA6D elisa kit  >1 publications with TREM2 and SEMA6D
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Other Semaphorin Interactions Pathway antibodies  Other Semaphorin Interactions Pathway Diagram
 Semaphorin Interactions Pathway antibodies  Semaphorin Interactions Pathway Diagram
Diseases associated with TREM2 elisa kitOrgans/Tissues associated with TREM2 elisa kit
 Disease Name  Pubmed Publications
 Dementia Antibodies  >89 publications with TREM2 and Dementia
 Nervous System Diseases Antibodies  >80 publications with TREM2 and Nervous System Diseases
 Alzheimer Disease Antibodies  >62 publications with TREM2 and Alzheimer Disease
 Inflammation Antibodies  >54 publications with TREM2 and Inflammation
 Polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy Antibodies  >23 publications with TREM2 and Polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy
 Disease Models, Animal Antibodies  >14 publications with TREM2 and Disease Models, Animal
 Necrosis Antibodies  >12 publications with TREM2 and Necrosis
 Skin Diseases Antibodies  >10 publications with TREM2 and Skin Diseases
 Lung Diseases Antibodies  >8 publications with TREM2 and Lung Diseases
 Memory Disorders Antibodies  >4 publications with TREM2 and Memory Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >74 publications with TREM2 and Brain
 Lung Antibodies  >12 publications with TREM2 and Lung
 Eye Antibodies  >2 publications with TREM2 and Eye
 Ovary Antibodies  >2 publications with TREM2 and Ovary
 Kidney Antibodies  >1 publications with TREM2 and Kidney
 Adipose Tissue Antibodies  >1 publications with TREM2 and Adipose Tissue
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