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TNNT1 elisa kit :: Human Troponin T, slow skeletal muscle ELISA Kit

Scan QR to view Datasheet Catalog #    MBS764955
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Troponin T, slow skeletal muscle (TNNT1), ELISA Kit

★Popular Item★
 Also Known As   

Human Troponin T, slow skeletal muscle ELISA Kit

 Product Synonym Names    TNNT1 (Troponin T Type 1; Slow Skeletal)/TNT/TnTs/sTnT/Slow skeletal muscle troponin T/Troponin T; slow skeletal muscle
 Product Gene Name   

TNNT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS764955 COA    COA PDF
 MBS764955 QC Report    QC Report PDF
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of TNNT1 . No significant cross-reactivity or interference between TNNT1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/ml
 Sensitivity    <9.375pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNNT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNNT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNNT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNNT1 purchase    MBS764955 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Troponin T, slow skeletal muscle (TNNT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/ml in biological research samples containing TNNT1, with an estimated sensitivity of <9.375pg/ml. The ELISA analytical biochemical technique of the MBS764955 kit is based on TNNT1 antibody-TNNT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNNT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNNT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNNT1 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TNNT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TNNT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNNT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNNT1 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of TNNT1 elisa kit    TNNT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS764955. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNNT1. It may not necessarily be applicable to this product.
 NCBI GI #    471270250
 NCBI GeneID    171409
 NCBI Accession #    NP_001264191.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001277262.2 [Other Products]
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 UniProt Secondary Accession #    Q7TNA9; Q7TNB0; Q7TNB1; Q923S7 [Other Products]
 UniProt Related Accession #    Q7TNB2 [Other Products]
 Molecular Weight    30,010 Da
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 NCBI Official Full Name    troponin T, slow skeletal muscle isoform 4
 NCBI Official Synonym Full Names    troponin T type 1 (skeletal, slow)
 NCBI Official Symbol    Tnnt1 [Similar Products]
 NCBI Official Synonym Symbols   
sTnT; tnTs; Fang2
[Similar Products]
 NCBI Protein Information    troponin T, slow skeletal muscle
 UniProt Protein Name    Troponin T, slow skeletal muscle
 UniProt Synonym Protein Names   
Slow skeletal muscle troponin T; sTnT
 Protein Family    Troponin
 UniProt Gene Name    Tnnt1 [Similar Products]
 UniProt Synonym Gene Names    Fang2; TnTs; sTnT [Similar Products]
 UniProt Entry Name    TNNT1_RAT
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 NCBI Summary for TNNT1    human homolog plays a role in the calcium sensitivity of contraction in skeletal muscle [RGD, Feb 2006]
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 UniProt Comments for TNNT1    TNNT1: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT1 are the cause of nemaline myopathy type 5 (NEM5); also known as Amish nemaline myopathy (ANM). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 5 is a severe and progressive form common among Old Order Amish. Affected infants display tremors with hypotonia and mild contractures of the shoulders and hips. Proximal contractures progressively weaken and a pectus carinatum deformity develops before children die of respiratory insufficiency, usually in the second year. Belongs to the troponin T family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Motility/polarity/chemotaxis

Cellular Component: troponin complex

Molecular Function: tropomyosin binding; troponin T binding

Biological Process: negative regulation of muscle contraction; regulation of muscle contraction; skeletal muscle contraction; slow-twitch skeletal muscle fiber contraction; transition between fast and slow fiber
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNNT1 elisa kitPathways associated with TNNT1 elisa kit
 Reference Product  PubMed Publications
 Tpm2 elisa kit  >11 publications with TNNT1 and Tpm2
 Tnnt2 elisa kit  >3 publications with TNNT1 and Tnnt2
 Tnni2 elisa kit  >3 publications with TNNT1 and Tnni2
 Tnni3 elisa kit  >2 publications with TNNT1 and Tnni3
 Myl3 elisa kit  >1 publications with TNNT1 and Myl3
 Tnnc1 elisa kit  >1 publications with TNNT1 and Tnnc1
 Tpm1 elisa kit  >1 publications with TNNT1 and Tpm1
 Tnni1 elisa kit  >1 publications with TNNT1 and Tnni1
 Products by Pathway  Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
Diseases associated with TNNT1 elisa kitOrgans/Tissues associated with TNNT1 elisa kit
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >4 publications with TNNT1 and Brain Diseases
 Disease Models, Animal Antibodies  >4 publications with TNNT1 and Disease Models, Animal
 Nemaline myopathy 5 Antibodies  >2 publications with TNNT1 and Nemaline myopathy 5
 Neurotoxicity Syndromes Antibodies  >1 publications with TNNT1 and Neurotoxicity Syndromes
 Heart Diseases Antibodies  >1 publications with TNNT1 and Heart Diseases
 Neoplasm Metastasis Antibodies  >1 publications with TNNT1 and Neoplasm Metastasis
 Weight Loss Antibodies  >1 publications with TNNT1 and Weight Loss
 Inflammation Antibodies  >1 publications with TNNT1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >30 publications with TNNT1 and Muscle
 Brain Antibodies  >5 publications with TNNT1 and Brain
 Embryonic Tissue Antibodies  >2 publications with TNNT1 and Embryonic Tissue
 Uncharacterized Tissue Antibodies  >1 publications with TNNT1 and Uncharacterized Tissue
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