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ULBP4 elisa kit :: Human UL16 Binding protein 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS744275
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

UL16 Binding protein 4 (ULBP4), ELISA Kit

 Also Known As   

Human UL16 Binding protein 4 ELISA Kit

 Product Gene Name   

ULBP4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609243
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of UL16BP4. No significant cross-reactivity or interference between UL16BP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between UL16BP4 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ULBP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ULBP4 purchase    MBS744275 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the UL16 Binding protein 4 (ULBP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ULBP4, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS744275 kit is based on ULBP4 antibody-ULBP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ULBP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ULBP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ULBP4 elisa kit

   Intended Uses: This UL16BP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human UL16BP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||UL16BP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UL16BP4 antibody and an UL16BP4-HRP conjugate. The assay sample and buffer are incubated together with UL16BP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UL16BP4 concentration since UL16BP4 from samples and UL16BP4-HRP conjugate compete for the anti-UL16BP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by UL16BP4 from the sample, fewer sites are left to bind UL16BP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UL16BP4 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ULBP4 elisa kit    Immunology
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 Typical Testing Data/Standard Curve of ULBP4 elisa kit    ULBP4 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS744275. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ULBP4. It may not necessarily be applicable to this product.
 NCBI GI #    31322668
 NCBI GeneID    135250
 NCBI Accession #    AAP15166.1 [Other Products]
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 UniProt Secondary Accession #    Q5VYB7; Q5VYB8; Q8TEZ2; Q96L41; A6YF59 [Other Products]
 UniProt Related Accession #    Q8TD07 [Other Products]
 Molecular Weight    23,978 Da
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 NCBI Official Full Name    ULBP4
 NCBI Official Synonym Full Names    retinoic acid early transcript 1E
 NCBI Official Symbol    RAET1E [Similar Products]
 NCBI Official Synonym Symbols   
RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
[Similar Products]
 NCBI Protein Information    NKG2D ligand 4; RAE-1-like transcript 4; lymphocyte effector toxicity activation ligand
 UniProt Protein Name    NKG2D ligand 4
 UniProt Synonym Protein Names   
Lymphocyte effector toxicity activation ligand; RAE-1-like transcript 4; RL-4; Retinoic acid early transcript 1E
 UniProt Gene Name    RAET1E [Similar Products]
 UniProt Synonym Gene Names    LETAL; N2DL4; ULBP4; UNQ1867/PRO4303; N2DL-4; NKG2DL4; RL-4 [Similar Products]
 UniProt Entry Name    N2DL4_HUMAN
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 NCBI Summary for ULBP4    This gene belong to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3. This and RAET1G protein differ from other RAET1 proteins in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. This protein functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
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 UniProt Comments for ULBP4    RAET1E: Ligand for the NKG2D receptor. Delivers activating signals to NK cells and promotes tumor immune surveillance by inducing the expansion of anti-tumor cytotoxic lymphocytes. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: extracellular region; plasma membrane; integral to membrane

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity; T cell mediated cytotoxicity
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 Research Articles on ULBP4    1. genetic polymorphism among the ethnic groups
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ULBP4 elisa kitPathways associated with ULBP4 elisa kit
 Reference Product  PubMed Publications
 KLRK1 elisa kit  >13 publications with ULBP4 and KLRK1
 RAET1G elisa kit  >8 publications with ULBP4 and RAET1G
 ULBP2 elisa kit  >7 publications with ULBP4 and ULBP2
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
Diseases associated with ULBP4 elisa kitOrgans/Tissues associated with ULBP4 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >2 publications with ULBP4 and Cardiovascular Diseases
 Brain Diseases Antibodies  >1 publications with ULBP4 and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with ULBP4 and Carcinoma, Hepatocellular
 Nervous System Diseases Antibodies  >1 publications with ULBP4 and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with ULBP4 and Liver Diseases
 Liver Neoplasms Antibodies  >1 publications with ULBP4 and Liver Neoplasms
 Necrosis Antibodies  >1 publications with ULBP4 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with ULBP4 and Liver
 Brain Antibodies  >1 publications with ULBP4 and Brain
 Skin Antibodies  >1 publications with ULBP4 and Skin
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