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UBE3A elisa kit :: Human Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2018879 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Standard Curve/Testing Data
Unit / Price
24-Strip-Wells  /  $260 +1 FREE 8GB USB
48-Strip-Wells  /  $475 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,590 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,670 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ubiquitin Protein Ligase E3A (UBE3A), ELISA Kit

★Popular Item★
 Also Known As   

Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit

 Product Synonym Names    ANCR; AS; E6-AP; EPVE6AP; HPVE6A; Papilloma Virus E6-Associated,Angelman Syndrome; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54
 Product Gene Name   

UBE3A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9VTH1
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Ubiquitin Protein Ligase E3A (UBE3A).
No significant cross-reactivity or interference between Ubiquitin Protein Ligase E3A (UBE3A) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.119ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Ubiquitin Protein Ligase E3A (UBE3A) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Ubiquitin Protein Ligase E3A (UBE3A) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of UBE3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for UBE3A purchase    MBS2018879 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing UBE3A, with an estimated sensitivity of < 0.119ng/mL. The ELISA analytical biochemical technique of the MBS2018879 kit is based on UBE3A antibody-UBE3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UBE3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UBE3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for UBE3A elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3A (UBE3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3A (UBE3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Protein Ligase E3A (UBE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Protein Ligase E3A (UBE3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for UBE3A elisa kit    Enzyme & Kinase; Developmental science
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 Typical Standard Curve/Testing Data of UBE3A elisa kit    UBE3A elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2018879. Click to request current manual
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NCBI/Uniprot data below describe general gene information for UBE3A. It may not necessarily be applicable to this product.
 NCBI GI #    21356487
 NCBI GeneID    39266
 NCBI Accession #    NP_648452.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_140195.4 [Other Products]
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 UniProt Primary Accession #    Q9VTH1 [Other Products]
 UniProt Related Accession #    Q9VTH1 [Other Products]
 Molecular Weight    107,590 Da
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 NCBI Official Full Name    ubiquitin protein ligase E3A
 NCBI Official Synonym Full Names    Ubiquitin protein ligase E3A
 NCBI Official Symbol    Ube3a [Similar Products]
 NCBI Official Synonym Symbols   
As; AS; CG6190; das; DmelCG6190; dube3a; dube3A; dUBE3A; Dube3a; ube3a; UBE3A
[Similar Products]
 NCBI Protein Information    CG6190 gene product from transcript CG6190-RA
 UniProt Protein Name    LD21888p
 UniProt Synonym Protein Names   
LD21888p
 Protein Family    Ubiquitin-protein ligase
 UniProt Gene Name    Ube3a [Similar Products]
 UniProt Entry Name    Q9VTH1_DROME
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 Research Articles on UBE3A    1. Data indicate that E3 ligase called ube3 (dube3a) is required for brain development as mutants have defective mushroom bodies.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with UBE3A elisa kitPathways associated with UBE3A elisa kit
 Reference Product  PubMed Publications
 p53 elisa kit  >71 publications with UBE3A and p53
 Pros54 elisa kit  >1 publications with UBE3A and Pros54
 pbl elisa kit  >1 publications with UBE3A and pbl
 Products by Pathway  Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
Diseases associated with UBE3A elisa kitOrgans/Tissues associated with UBE3A elisa kit
 Disease Name  Pubmed Publications
 Angelman Syndrome Antibodies  >281 publications with UBE3A and Angelman Syndrome
 Neurobehavioral Manifestations Antibodies  >98 publications with UBE3A and Neurobehavioral Manifestations
 Epilepsy Antibodies  >45 publications with UBE3A and Epilepsy
 Autistic Disorder Antibodies  >34 publications with UBE3A and Autistic Disorder
 Uterine Cervical Neoplasms Antibodies  >21 publications with UBE3A and Uterine Cervical Neoplasms
 Learning Disorders Antibodies  >10 publications with UBE3A and Learning Disorders
 Cognition Disorders Antibodies  >8 publications with UBE3A and Cognition Disorders
 Adenocarcinoma Antibodies  >7 publications with UBE3A and Adenocarcinoma
 Breast Neoplasms Antibodies  >6 publications with UBE3A and Breast Neoplasms
 Nervous System Malformations Antibodies  >5 publications with UBE3A and Nervous System Malformations
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with UBE3A and Blood
 Fat Body Antibodies  >3 publications with UBE3A and Fat Body
 Testis Antibodies  >2 publications with UBE3A and Testis
 Uncharacterized Tissue Antibodies  >1 publications with UBE3A and Uncharacterized Tissue
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