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ISG15 elisa kit :: Human Ubiquitin-like modifier ELISA Kit

Scan QR to view Datasheet Catalog #    MBS283284 ISG15 elisa kit
Unit / Price
48-Strip-Wells  /  $465 +1 FREE 8GB USB
96-Strip-Wells  /  $790 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,500 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,655 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ubiquitin-like modifier (ISG15), ELISA Kit

★Popular Item★
 Also Known As   

Human Ubiquitin-like modifier (ISG15) ELISA Kit

 Product Gene Name   

ISG15 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    M13755 mRNA
 3D Structure    ModBase 3D Structure for P05161
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ISG15. No significant cross-reactivity or interference between Human ISG15 and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ISG15 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ISG15 purchase    MBS283284 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ubiquitin-like modifier (ISG15) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ISG15. The ELISA analytical biochemical technique of the MBS283284 kit is based on ISG15 antibody-ISG15 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ISG15 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ISG15. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ISG15 elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ISG15 in samples. An antibody specific for ISG15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyISG15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ISG15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ISG15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for ISG15. It may not necessarily be applicable to this product.
 NCBI GI #    4826774
 NCBI GeneID    9636
 NCBI Accession #    NP_005092.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005101.3 [Other Products]
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 UniProt Primary Accession #    P05161 [Other Products]
 UniProt Secondary Accession #    Q5SVA4; Q7Z2G2; Q96GF0 [Other Products]
 UniProt Related Accession #    P05161 [Other Products]
 Molecular Weight    17,888 Da
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 NCBI Official Full Name    ubiquitin-like protein ISG15
 NCBI Official Synonym Full Names    ISG15 ubiquitin-like modifier
 NCBI Official Symbol    ISG15 [Similar Products]
 NCBI Official Synonym Symbols   
G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
[Similar Products]
 NCBI Protein Information    ubiquitin-like protein ISG15
 UniProt Protein Name    Ubiquitin-like protein ISG15
 UniProt Synonym Protein Names   
Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP
 Protein Family    Ubiquitin-like protein
 UniProt Gene Name    ISG15 [Similar Products]
 UniProt Synonym Gene Names    G1P2; UCRP; IP17; hUCRP [Similar Products]
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 NCBI Summary for ISG15    The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]
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 UniProt Comments for ISG15    ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytosol; extracellular region; nucleoplasm

Molecular Function: protein binding; protein tag

Biological Process: bypass DNA synthesis; defense response to bacterium; defense response to virus; ISG15-protein conjugation; modification-dependent protein catabolic process; negative regulation of protein ubiquitination; negative regulation of type I interferon production; negative regulation of viral genome replication; positive regulation of bone mineralization; positive regulation of erythrocyte differentiation; regulation of interferon-gamma production; viral process

Disease: Immunodeficiency 38
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 Research Articles on ISG15    1. findings revealed that MERS-CoV PLpro chiefly engages human ISG15 through its C-terminal domain; structure of MERS-CoV PLpro was solved to 2.4 A in complex with the C-terminal domain of hISG15; structure of MERS-CoV PLpro in complex with this domain exposed the interface between these two entities
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ISG15 elisa kitPathways associated with ISG15 elisa kit
 Reference Product  PubMed Publications
 MX1 elisa kit  >41 publications with ISG15 and MX1
 IRF7 elisa kit  >25 publications with ISG15 and IRF7
 IFIT1 elisa kit  >22 publications with ISG15 and IFIT1
 HERC5 elisa kit  >22 publications with ISG15 and HERC5
 DDX58 elisa kit  >13 publications with ISG15 and DDX58
 IFI6 elisa kit  >8 publications with ISG15 and IFI6
 IFIT2 elisa kit  >8 publications with ISG15 and IFIT2
 IFIT3 elisa kit  >6 publications with ISG15 and IFIT3
 ISG20 elisa kit  >5 publications with ISG15 and ISG20
 UBA7 elisa kit  >1 publications with ISG15 and UBA7
 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Interferon Alpha/beta Signaling Pathway antibodies  Interferon Alpha/beta Signaling Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
Diseases associated with ISG15 elisa kitOrgans/Tissues associated with ISG15 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >70 publications with ISG15 and Neoplasms
 Liver Diseases Antibodies  >45 publications with ISG15 and Liver Diseases
 Inflammation Antibodies  >25 publications with ISG15 and Inflammation
 Influenza, Human Antibodies  >25 publications with ISG15 and Influenza, Human
 Necrosis Antibodies  >24 publications with ISG15 and Necrosis
 Kidney Diseases Antibodies  >15 publications with ISG15 and Kidney Diseases
 Breast Neoplasms Antibodies  >12 publications with ISG15 and Breast Neoplasms
 Disease Models, Animal Antibodies  >12 publications with ISG15 and Disease Models, Animal
 Lung Diseases Antibodies  >11 publications with ISG15 and Lung Diseases
 Cell Transformation, Neoplastic Antibodies  >10 publications with ISG15 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >103 publications with ISG15 and Blood
 Liver Antibodies  >49 publications with ISG15 and Liver
 Kidney Antibodies  >33 publications with ISG15 and Kidney
 Lung Antibodies  >31 publications with ISG15 and Lung
 Uterus Antibodies  >25 publications with ISG15 and Uterus
 Brain Antibodies  >24 publications with ISG15 and Brain
 Bone Antibodies  >16 publications with ISG15 and Bone
 Placenta Antibodies  >11 publications with ISG15 and Placenta
 Skin Antibodies  >9 publications with ISG15 and Skin
 Embryonic Tissue Antibodies  >8 publications with ISG15 and Embryonic Tissue
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