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USH2A elisa kit :: Human Usherin, USH2A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2602743
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Usherin, USH2A, ELISA Kit

★Popular Item★
 Also Known As   

Human Usherin, USH2A ELISA Kit

 Product Gene Name   

USH2A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 613809
 3D Structure    ModBase 3D Structure for O75445
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    human serum, plasma or cell culture supernatant
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/mL
 Intra-assay Precision    ? 8%
 Inter-assay Precision    ? 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of USH2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for USH2A purchase    MBS2602743 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Usherin, USH2A, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing USH2A, with an estimated sensitivity of 0.06 ng/mL. The ELISA analytical biochemical technique of the MBS2602743 kit is based on USH2A antibody-USH2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect USH2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, USH2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as human serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for USH2A elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human USH2A monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of USH2A elisa kit    USH2A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2602743. Click to request current manual
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NCBI/Uniprot data below describe general gene information for USH2A. It may not necessarily be applicable to this product.
 NCBI GI #    219842259
 NCBI GeneID    7399
 NCBI Accession #    NP_009054.5 [Other Products]
 NCBI GenBank Nucleotide #    NM_007123.5 [Other Products]
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 UniProt Primary Accession #    O75445 [Other Products]
 UniProt Secondary Accession #    Q5VVM9; Q6S362; Q9NS27 [Other Products]
 UniProt Related Accession #    O75445 [Other Products]
 Molecular Weight    575,600 Da
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 NCBI Official Full Name    usherin isoform A
 NCBI Official Synonym Full Names    Usher syndrome 2A (autosomal recessive, mild)
 NCBI Official Symbol    USH2A [Similar Products]
 NCBI Official Synonym Symbols   
US2; RP39; USH2; dJ1111A8.1
[Similar Products]
 NCBI Protein Information    usherin; usher syndrome type-2A protein; usher syndrome type IIa protein
 UniProt Protein Name    Usherin
 UniProt Synonym Protein Names   
Usher syndrome type IIa protein; Usher syndrome type-2A protein
 Protein Family    Usherin
 UniProt Gene Name    USH2A [Similar Products]
 UniProt Entry Name    USH2A_HUMAN
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 NCBI Summary for USH2A    This gene encodes a protein that contains laminin EGF motifs, a pentaxin domain, and many fibronectin type III motifs. The protein is found in the basement membrane, and may be important in development and homeostasis of the inner ear and retina. Mutations within this gene have been associated with Usher syndrome type IIa and retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
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 UniProt Comments for USH2A    USH2A: Involved in hearing and vision. Defects in USH2A are the cause of Usher syndrome type 2A (USH2A). USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH2 is characterized by congenital mild hearing impairment with normal vestibular responses. Defects in USH2A are the cause of retinitis pigmentosa type 39 (RP39). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP39 inheritance is autosomal recessive. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: photoreceptor inner segment; stereocilium bundle; cytoplasm; apical plasma membrane; integral to membrane; basement membrane; photoreceptor connecting cilium

Molecular Function: collagen binding; protein binding; myosin binding

Biological Process: sensory perception of sound; visual perception; inner ear receptor cell differentiation; sensory perception of light stimulus; photoreceptor cell maintenance; response to stimulus; hair cell differentiation; maintenance of organ identity

Disease: Retinitis Pigmentosa 39; Usher Syndrome, Type Iia
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 Research Articles on USH2A    1. Here, we report an effect of the common c.2299delG mutation on splicing of exons 12 and 13 of USH2A.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with USH2A elisa kitOrgans/Tissues associated with USH2A elisa kit
 Disease Name  Pubmed Publications
 Retinitis Pigmentosa Antibodies  >102 publications with USH2A and Retinitis Pigmentosa
 Usher syndrome, type 2A Antibodies  >4 publications with USH2A and Usher syndrome, type 2A
 Optic Nerve Diseases Antibodies  >2 publications with USH2A and Optic Nerve Diseases
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >32 publications with USH2A and Eye
 Brain Antibodies  >5 publications with USH2A and Brain
 Liver Antibodies  >2 publications with USH2A and Liver
 Testis Antibodies  >2 publications with USH2A and Testis
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