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RELB elisa kit :: Human V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022354 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $355 +1 FREE 8GB USB
48-Strip-Wells  /  $540 +1 FREE 8GB USB
96-Strip-Wells  /  $730 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,985 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,410 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB), ELISA Kit

 Also Known As   

V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) ELISA Kit

 Product Synonym Names    REL-B; I-REL; Transcription Factor RelB; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3
 Product Gene Name   

RELB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    604758
 3D Structure    ModBase 3D Structure for Q01201
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB).
No significant cross-reactivity or interference between V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) and analogues was observed.
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 Samples    Tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Typically less than 0.063ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RELB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RELB purchase    MBS2022354 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing RELB, with an estimated sensitivity of Typically less than 0.063ng/mL. The ELISA analytical biochemical technique of the MBS2022354 kit is based on RELB antibody-RELB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RELB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RELB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for RELB elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of RELB elisa kit    RELB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022354. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RELB. It may not necessarily be applicable to this product.
 NCBI GI #    20380825
 NCBI GeneID    5971
 NCBI Accession #    AAH28013.1 [Other Products]
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 UniProt Primary Accession #    Q01201 [Other Products]
 UniProt Secondary Accession #    Q6GTX7; Q9UEI7 [Other Products]
 UniProt Related Accession #    Q01201 [Other Products]
 Molecular Weight    62,134 Da
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 NCBI Official Full Name    V-rel reticuloendotheliosis viral oncogene homolog B
 NCBI Official Synonym Full Names    v-rel avian reticuloendotheliosis viral oncogene homolog B
 NCBI Official Symbol    RELB [Similar Products]
 NCBI Official Synonym Symbols   
IREL; I-REL; REL-B
[Similar Products]
 NCBI Protein Information    transcription factor RelB
 UniProt Protein Name    Transcription factor RelB
 UniProt Synonym Protein Names   
I-Rel
 Protein Family    Antitoxin
 UniProt Gene Name    RELB [Similar Products]
 UniProt Entry Name    RELB_HUMAN
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 UniProt Comments for RELB    RELB: a transcription factor of the nuclear factor-kappaB (NFkB) transcription complex. Stimulates promoter activity in the presence NFkB1/NFkB-p50. Plays role in regulation of apoptosis in hepatocellular carcinoma through activation of downstream target genes. Does not associate with DNA or RelA/NFkB-p65.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center; cytosol

Molecular Function: protein binding; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; I-kappaB kinase/NF-kappaB cascade; antigen processing and presentation; response to cytokine stimulus; T-helper 1 cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell differentiation; circadian regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; inflammatory response
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 Research Articles on RELB    1. RelB may represent a novel marker of health outcomes
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RELB elisa kitPathways associated with RELB elisa kit
 Reference Product  PubMed Publications
 NFKB2 elisa kit  >100 publications with RELB and NFKB2
 IKBKB elisa kit  >76 publications with RELB and IKBKB
 CHUK elisa kit  >32 publications with RELB and CHUK
 NFKB1 elisa kit  >32 publications with RELB and NFKB1
 BCL3 elisa kit  >8 publications with RELB and BCL3
 NFKBIA elisa kit  >7 publications with RELB and NFKBIA
 MAP3K14 elisa kit  >3 publications with RELB and MAP3K14
 JAK2 elisa kit  >2 publications with RELB and JAK2
 RAC1 elisa kit  >1 publications with RELB and RAC1
 Products by Pathway  Pathway Diagram
 Alternative NF-kappaB Pathway antibodies  Alternative NF-kappaB Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 NF-kappa B Signaling Pathway antibodies  NF-kappa B Signaling Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
Diseases associated with RELB elisa kitOrgans/Tissues associated with RELB elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >200 publications with RELB and Necrosis
 Inflammation Antibodies  >147 publications with RELB and Inflammation
 Breast Neoplasms Antibodies  >33 publications with RELB and Breast Neoplasms
 Disease Models, Animal Antibodies  >32 publications with RELB and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >21 publications with RELB and Cell Transformation, Neoplastic
 Cardiovascular Diseases Antibodies  >19 publications with RELB and Cardiovascular Diseases
 Lung Diseases Antibodies  >18 publications with RELB and Lung Diseases
 Kidney Diseases Antibodies  >14 publications with RELB and Kidney Diseases
 Liver Diseases Antibodies  >14 publications with RELB and Liver Diseases
 Neoplasms, Experimental Antibodies  >12 publications with RELB and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >291 publications with RELB and Blood
 Bone Marrow Antibodies  >73 publications with RELB and Bone Marrow
 Spleen Antibodies  >51 publications with RELB and Spleen
 Thymus Antibodies  >40 publications with RELB and Thymus
 Lung Antibodies  >37 publications with RELB and Lung
 Vascular Antibodies  >34 publications with RELB and Vascular
 Lymph Node Antibodies  >31 publications with RELB and Lymph Node
 Kidney Antibodies  >31 publications with RELB and Kidney
 Lymph Antibodies  >30 publications with RELB and Lymph
 Muscle Antibodies  >28 publications with RELB and Muscle
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