Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow VSIG4 rightarrow Human VSIG4  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Proteasome Subunit Beta Type 6 (PSMbeta6) ELISA Kit
 • Interleukin 8 (IL-8) Antibody
 • ABCG2 Antibody
 • Neurofascin (NFASC) Recombinant Protein
 • Vaccinia Virus Antibody
 • Anti Growth Hormone Antibody (GH) ELISA Kit
 • pre-B-cell leukemia homeobox 1 (PBX1) ELISA Kit
 • Antithrombin (AT) ELISA Kit
 • Egl nine homolog 1 (EGLN1) Antibody
 • ATG7 Antibody
 • GPIHBP1 Antibody
 • endothelin converting enzyme 1 (ECE1) ELISA Kit
 • Fibrillin 1 (FBN1) ELISA Kit
 • Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

VSIG4 elisa kit :: Human V-set and immunoglobulin domain containing 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS925833
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

V-set and immunoglobulin domain containing 4 (VSIG4), ELISA Kit

★Popular Item★
 Also Known As   

Human V-set and immunoglobulin domain-containing protein 4, VSIG4 ELISA Kit

 Product Synonym Names    Human V-set and immunoglobulin domain-containing protein 4 (VSIG4) ELISA kit; RP6-159A1.1; CRIg; Z39IG; Ig superfamily protein; complement receptor of the immunoglobulin superfamily; V-set and immunoglobulin domain containing 4
 Product Gene Name   

VSIG4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS925833 Testing Data    Testing Data PDF
 OMIM    300353
 3D Structure    ModBase 3D Structure for Q9Y279
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of human VSIG4. No significant cross-reactivity or interference between human VSIG4 and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of human VSIG4 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VSIG4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VSIG4 purchase    MBS925833 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the V-set and immunoglobulin domain containing 4 (VSIG4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing VSIG4, with an estimated sensitivity of The minimum detectable dose of human VSIG4 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS925833 kit is based on VSIG4 antibody-VSIG4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VSIG4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VSIG4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for VSIG4 elisa kit

  
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VSIG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VSIG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VSIG4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VSIG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of VSIG4 elisa kit    VSIG4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS925833. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for VSIG4. It may not necessarily be applicable to this product.
 NCBI GI #    154426280
 NCBI GeneID    11326
 NCBI Accession #    NP_001093901.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001100431.1 [Other Products]
Section Bar
 UniProt Primary Accession #    Q9Y279 [Other Products]
 UniProt Secondary Accession #    Q6UXI4 [Other Products]
 UniProt Related Accession #    Q9Y279 [Other Products]
 Molecular Weight    43,987 Da
Table BarTOPTable Bar
 NCBI Official Full Name    V-set and immunoglobulin domain-containing protein 4 isoform 2
 NCBI Official Synonym Full Names    V-set and immunoglobulin domain containing 4
 NCBI Official Symbol    VSIG4 [Similar Products]
 NCBI Official Synonym Symbols   
CRIg; Z39IG
[Similar Products]
 NCBI Protein Information    V-set and immunoglobulin domain-containing protein 4; protein Z39Ig; Ig superfamily protein; complement receptor of the immunoglobulin superfamily
 UniProt Protein Name    V-set and immunoglobulin domain-containing protein 4
 UniProt Synonym Protein Names   
Protein Z39Ig
 Protein Family    V-set and immunoglobulin domain-containing protein
 UniProt Gene Name    VSIG4 [Similar Products]
 UniProt Synonym Gene Names    CRIg; Z39IG; UNQ317/PRO362 [Similar Products]
 UniProt Entry Name    VSIG4_HUMAN
Section Bar
 NCBI Summary for VSIG4    This gene encodes a v-set and immunoglobulin-domain containing protein that is structurally related to the B7 family of immune regulatory proteins. The encoded protein may be a negative regulator of T-cell responses. This protein is also a receptor for the complement component 3 fragments C3b and iC3b. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
Section Bar
 UniProt Comments for VSIG4    VSIG4: Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq12-q13.3

Cellular Component: integral to membrane

Molecular Function: protein binding

Biological Process: negative regulation of T cell proliferation; complement activation, alternative pathway; negative regulation of interleukin-2 production
Table BarTOPTable Bar
 Research Articles on VSIG4    1. we showed that a complement receptor of the Ig superfamily (CRIg, also known as Z39Ig), a receptor for complement fragments (C3b and iC3b), was expressed on a subset of intestinal macrophages in murine and human large intestine
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Diseases associated with VSIG4 elisa kitOrgans/Tissues associated with VSIG4 elisa kit
 Disease Name  Pubmed Publications
 Arthritis, Rheumatoid Antibodies  >3 publications with VSIG4 and Arthritis, Rheumatoid
 Inflammation Antibodies  >3 publications with VSIG4 and Inflammation
 Cardiovascular Diseases Antibodies  >2 publications with VSIG4 and Cardiovascular Diseases
 Hepatitis Antibodies  >2 publications with VSIG4 and Hepatitis
 Kidney Diseases Antibodies  >2 publications with VSIG4 and Kidney Diseases
 Liver Diseases Antibodies  >2 publications with VSIG4 and Liver Diseases
 Arteriosclerosis Antibodies  >1 publications with VSIG4 and Arteriosclerosis
 Aneuploidy Antibodies  >1 publications with VSIG4 and Aneuploidy
 Neoplasms Antibodies  >1 publications with VSIG4 and Neoplasms
 Cardiomyopathies Antibodies  >1 publications with VSIG4 and Cardiomyopathies
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with VSIG4 and Blood
 Liver Antibodies  >3 publications with VSIG4 and Liver
 Placenta Antibodies  >3 publications with VSIG4 and Placenta
 Kidney Antibodies  >2 publications with VSIG4 and Kidney
 Lung Antibodies  >2 publications with VSIG4 and Lung
 Embryonic Tissue Antibodies  >1 publications with VSIG4 and Embryonic Tissue
 Umbilical Cord Antibodies  >1 publications with VSIG4 and Umbilical Cord
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions