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VCAM1 elisa kit :: Human VCAM-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175995
 Typical Testing Data/Standard Curve
Unit / Price
96-Strip-Wells  /  $360 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VCAM-1 (VCAM1), ELISA Kit

★Popular Item★
 Also Known As   

Human VCAM-1 PicoKine ELISA Kit

 Product Synonym Names    Vascular cell adhesion protein 1; vascular cell adhesion molecule 1; CD106; CD106 antigen; DKFZp779G2333; INCAM 100; INCAM-100; INCAM100; L1CAM; MGC99561; V CAM 1; V-CAM 1; Vascular cell adhesion molecule 1; Vascular cell adhesion protein 1; VCAM 1; VCAM-1; VCAM1; VCAM1_HUMAN
 Product Gene Name   

VCAM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    192225
 3D Structure    ModBase 3D Structure for P19320
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 Species Reactivity    Human
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 Specificity    Natural and recombinant human VCAM-1
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 Samples    Cell culture supernates, serum and plasma (heparin, EDTA)
 Assay Type    Sandwich
 Detection Range    156pg/ml-10,000pg/ml
 Sensitivity    < 10pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VCAM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VCAM1 purchase    MBS175995 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the VCAM-1 (VCAM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 156pg/ml-10,000pg/ml in biological research samples containing VCAM1, with an estimated sensitivity of < 10pg/ml. The ELISA analytical biochemical technique of the MBS175995 kit is based on VCAM1 antibody-VCAM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCAM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCAM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for VCAM1 elisa kit

   Principle of the assay: human VCAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VCAM-1 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VCAM-1 amount of sample captured in plate.
Background: Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.1 Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions,making its expression and function a target for therapeutic intervention.2 Integrin alpha4beta1 (VLA-4) and VCAM-1facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.3The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.1 The standard product used in this kit is recombinant human VCAM-1,consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.
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  Typical Testing Data/Standard Curve of VCAM1 elisa kit    Human VCAM-1 PicoKine ELISA Kit standard curve
VCAM1 elisa kit  Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS175995. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VCAM1. It may not necessarily be applicable to this product.
 NCBI GI #    137560
 NCBI GeneID    7412
 NCBI Accession #    P19320.1 [Other Products]
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 UniProt Primary Accession #    P19320 [Other Products]
 UniProt Secondary Accession #    Q6NUP8; A8K6R7; B4DKS4; E9PDD1 [Other Products]
 UniProt Related Accession #    P19320 [Other Products]
 Molecular Weight    74,346 Da
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 NCBI Official Full Name    Vascular cell adhesion protein 1
 NCBI Official Synonym Full Names    vascular cell adhesion molecule 1
 NCBI Official Symbol    VCAM1 [Similar Products]
 NCBI Official Synonym Symbols   
CD106; INCAM-100
[Similar Products]
 NCBI Protein Information    vascular cell adhesion protein 1; CD106 antigen
 UniProt Protein Name    Vascular cell adhesion protein 1
 UniProt Synonym Protein Names   
INCAM-100; CD_antigen: CD106
 Protein Family    Vascular cell adhesion protein
 UniProt Gene Name    VCAM1 [Similar Products]
 UniProt Synonym Gene Names    L1CAM; V-CAM 1; VCAM-1 [Similar Products]
 UniProt Entry Name    VCAM1_HUMAN
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 NCBI Summary for VCAM1    This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
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 UniProt Comments for VCAM1    VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: Golgi apparatus; extracellular space; cell surface; microvillus; endoplasmic reticulum; early endosome; integral to membrane; apical part of cell; plasma membrane; podosome; sarcolemma; filopodium; external side of plasma membrane

Molecular Function: amine oxidase activity; integrin binding; cell adhesion molecule binding

Biological Process: response to nicotine; regulation of immune response; chronic inflammatory response; extracellular matrix organization and biogenesis; viral reproduction; heart development; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; B cell differentiation; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; amine metabolic process; response to ionizing radiation; cell adhesion; leukocyte tethering or rolling; acute inflammatory response; response to nutrient; aging
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 Research Articles on VCAM1    1. Serum ICAM and VCAM levels are higher in first episode mania than those in subsequent remission and healthy individuals.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VCAM1 elisa kitPathways associated with VCAM1 elisa kit
 Reference Product  PubMed Publications
 CD44 elisa kit  >14 publications with VCAM1 and CD44
 ITGA4 elisa kit  >10 publications with VCAM1 and ITGA4
 ITGB2 elisa kit  >6 publications with VCAM1 and ITGB2
 VEGFA elisa kit  >3 publications with VCAM1 and VEGFA
 ITGB1 elisa kit  >3 publications with VCAM1 and ITGB1
 SRC elisa kit  >2 publications with VCAM1 and SRC
 ITGAD elisa kit  >1 publications with VCAM1 and ITGAD
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cellular Roles Of Anthrax Toxin Pathway antibodies  Cellular Roles Of Anthrax Toxin Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
Diseases associated with VCAM1 elisa kitOrgans/Tissues associated with VCAM1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >76 publications with VCAM1 and Inflammation
 Cardiovascular Diseases Antibodies  >52 publications with VCAM1 and Cardiovascular Diseases
 Necrosis Antibodies  >47 publications with VCAM1 and Necrosis
 Atherosclerosis Antibodies  >38 publications with VCAM1 and Atherosclerosis
 Nervous System Diseases Antibodies  >19 publications with VCAM1 and Nervous System Diseases
 Kidney Diseases Antibodies  >13 publications with VCAM1 and Kidney Diseases
 Hypertension Antibodies  >12 publications with VCAM1 and Hypertension
 Heart Diseases Antibodies  >10 publications with VCAM1 and Heart Diseases
 Myocardial Ischemia Antibodies  >9 publications with VCAM1 and Myocardial Ischemia
 Anemia Antibodies  >8 publications with VCAM1 and Anemia
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >190 publications with VCAM1 and Vascular
 Blood Antibodies  >117 publications with VCAM1 and Blood
 Kidney Antibodies  >23 publications with VCAM1 and Kidney
 Embryonic Tissue Antibodies  >21 publications with VCAM1 and Embryonic Tissue
 Brain Antibodies  >17 publications with VCAM1 and Brain
 Liver Antibodies  >14 publications with VCAM1 and Liver
 Heart Antibodies  >14 publications with VCAM1 and Heart
 Lung Antibodies  >10 publications with VCAM1 and Lung
 Skin Antibodies  >8 publications with VCAM1 and Skin
 Lymph Node Antibodies  >7 publications with VCAM1 and Lymph Node
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