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VCAM1 elisa kit :: Human Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2701976
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $190 +1 FREE 8GB USB
48-Strip-Wells  /  $285 +1 FREE 8GB USB
96-Strip-Wells  /  $355 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,495 +1 FREE 8GB USB
10x96-Strip-Wells  /  $2,765 +3 FREE 8GB USB
 
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 Product Name   

Vascular Cell Adhesion Molecule 1 (VCAM1), ELISA Kit

★Popular Item★
 Also Known As   

Human Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

 Product Synonym Names    CD106; INCAM-100; L1CAM
 Product Gene Name   

VCAM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    M30257 mRNA
 3D Structure    ModBase 3D Structure for P19320
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1).
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
 Detection Range    0.78-50ng/mL
 Sensitivity    < 0.29ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VCAM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VCAM1 purchase    MBS2701976 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78-50ng/mL in biological research samples containing VCAM1, with an estimated sensitivity of < 0.29ng/mL. The ELISA analytical biochemical technique of the MBS2701976 kit is based on VCAM1 antibody-VCAM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCAM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCAM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for VCAM1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Cell Adhesion Molecule 1 (VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of VCAM1 elisa kit    VCAM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2701976. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VCAM1. It may not necessarily be applicable to this product.
 NCBI GI #    179886
 NCBI GeneID    7412
 NCBI Accession #    AAA51917.1 [Other Products]
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 UniProt Primary Accession #    P19320 [Other Products]
 UniProt Secondary Accession #    Q6NUP8; A8K6R7; B4DKS4; E9PDD1 [Other Products]
 UniProt Related Accession #    P19320 [Other Products]
 Molecular Weight    74,346 Da
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 NCBI Official Full Name    vascular cell adhesion molecule 1
 NCBI Official Synonym Full Names    vascular cell adhesion molecule 1
 NCBI Official Symbol    VCAM1 [Similar Products]
 NCBI Official Synonym Symbols   
CD106; INCAM-100
[Similar Products]
 NCBI Protein Information    vascular cell adhesion protein 1
 UniProt Protein Name    Vascular cell adhesion protein 1
 UniProt Synonym Protein Names   
INCAM-100; CD_antigen: CD106
 Protein Family    Vascular cell adhesion protein
 UniProt Gene Name    VCAM1 [Similar Products]
 UniProt Synonym Gene Names    V-CAM 1; VCAM-1 [Similar Products]
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 NCBI Summary for VCAM1    This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
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 UniProt Comments for VCAM1    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
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 Research Articles on VCAM1    1. VCAM1 protein expression increased in cartilage tissue from spinal tuberculosis patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VCAM1 elisa kitPathways associated with VCAM1 elisa kit
 Reference Product  PubMed Publications
 CD44 elisa kit  >14 publications with VCAM1 and CD44
 ITGA4 elisa kit  >10 publications with VCAM1 and ITGA4
 ITGB2 elisa kit  >6 publications with VCAM1 and ITGB2
 VEGFA elisa kit  >3 publications with VCAM1 and VEGFA
 ITGB1 elisa kit  >3 publications with VCAM1 and ITGB1
 SRC elisa kit  >2 publications with VCAM1 and SRC
 ITGAD elisa kit  >1 publications with VCAM1 and ITGAD
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cellular Roles Of Anthrax Toxin Pathway antibodies  Cellular Roles Of Anthrax Toxin Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
Diseases associated with VCAM1 elisa kitOrgans/Tissues associated with VCAM1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >76 publications with VCAM1 and Inflammation
 Cardiovascular Diseases Antibodies  >52 publications with VCAM1 and Cardiovascular Diseases
 Necrosis Antibodies  >47 publications with VCAM1 and Necrosis
 Atherosclerosis Antibodies  >38 publications with VCAM1 and Atherosclerosis
 Nervous System Diseases Antibodies  >19 publications with VCAM1 and Nervous System Diseases
 Kidney Diseases Antibodies  >13 publications with VCAM1 and Kidney Diseases
 Hypertension Antibodies  >12 publications with VCAM1 and Hypertension
 Heart Diseases Antibodies  >10 publications with VCAM1 and Heart Diseases
 Myocardial Ischemia Antibodies  >9 publications with VCAM1 and Myocardial Ischemia
 Anemia Antibodies  >8 publications with VCAM1 and Anemia
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >190 publications with VCAM1 and Vascular
 Blood Antibodies  >117 publications with VCAM1 and Blood
 Kidney Antibodies  >23 publications with VCAM1 and Kidney
 Embryonic Tissue Antibodies  >21 publications with VCAM1 and Embryonic Tissue
 Brain Antibodies  >17 publications with VCAM1 and Brain
 Liver Antibodies  >14 publications with VCAM1 and Liver
 Heart Antibodies  >14 publications with VCAM1 and Heart
 Lung Antibodies  >10 publications with VCAM1 and Lung
 Skin Antibodies  >8 publications with VCAM1 and Skin
 Lymph Node Antibodies  >7 publications with VCAM1 and Lymph Node
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