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VAChT elisa kit :: Human Vesicular Acetylcholine Transporter (VAChT) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024606 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $380 +1 FREE 8GB USB
48-Strip-Wells  /  $565 +1 FREE 8GB USB
96-Strip-Wells  /  $765 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,130 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,670 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Vesicular Acetylcholine Transporter (VAChT), ELISA Kit

 Also Known As   

Vesicular Acetylcholine Transporter (VAChT) ELISA Kit

 Product Synonym Names    SLC18A3; Solute Carrier Family 18 Member 3,Vesicular Acetylcholine
 Product Gene Name   

VAChT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    600336
 3D Structure    ModBase 3D Structure for Q16572
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of VAChT. No significant cross-reactivity or interference between VAChT and analogues was observed.
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 Samples    Human tissue homogenates and other biological fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    The minimum detectable dose of VAChT is typically less than 0.117ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VAChT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VAChT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
 Intended Uses    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VAChT in human tissue homogenates and other biological fluids.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of VAChT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VAChT purchase    MBS2024606 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vesicular Acetylcholine Transporter (VAChT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing VAChT, with an estimated sensitivity of The minimum detectable dose of VAChT is typically less than 0.117ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. The ELISA analytical biochemical technique of the MBS2024606 kit is based on VAChT antibody-VAChT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VAChT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VAChT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for VAChT elisa kit

   Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VAChT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VAChT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VAChT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VAChT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of VAChT elisa kit    VAChT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024606
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NCBI/Uniprot data below describe general gene information for VAChT. It may not necessarily be applicable to this product.
 NCBI GI #    118582257
 NCBI GeneID    6572
 NCBI Accession #    NP_003046.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003055.2 [Other Products]
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 UniProt Primary Accession #    Q16572 [Other Products]
 UniProt Secondary Accession #    B2R7S1 [Other Products]
 UniProt Related Accession #    Q16572 [Other Products]
 Molecular Weight    56,903 Da
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 NCBI Official Full Name    vesicular acetylcholine transporter
 NCBI Official Synonym Full Names    solute carrier family 18 (vesicular acetylcholine transporter), member 3
 NCBI Official Symbol    SLC18A3 [Similar Products]
 NCBI Official Synonym Symbols   
VACHT
[Similar Products]
 NCBI Protein Information    vesicular acetylcholine transporter
 UniProt Protein Name    Vesicular acetylcholine transporter
 UniProt Synonym Protein Names   
Solute carrier family 18 member 3
 Protein Family    Vesicular acetylcholine transporter
 UniProt Gene Name    SLC18A3 [Similar Products]
 UniProt Synonym Gene Names    VACHT; VAChT [Similar Products]
 UniProt Entry Name    VACHT_HUMAN
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 NCBI Summary for VAChT    This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for VAChT    SLC18A3: Involved in acetylcholine transport into synaptic vesicles. Belongs to the major facilitator superfamily. Vesicular transporter family.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: acetylcholine transmembrane transporter activity

Biological Process: synaptic transmission; acetylcholine transport; neurotransmitter secretion
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 Research Articles on VAChT    1. PCMC expression of ADAM29, FLRT2, and SLC18A3 could be assessed as part of a routine screen to help identify individuals at risk of severe Obstructive sleep apnea in Asian populations
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VAChT elisa kitPathways associated with VAChT elisa kit
 Reference Product  PubMed Publications
 CHAT elisa kit  >130 publications with VAChT and CHAT
 ACHE elisa kit  >23 publications with VAChT and ACHE
 RAB3A elisa kit  >2 publications with VAChT and RAB3A
 Products by Pathway  Pathway Diagram
 Acetylcholine Neurotransmitter Release Cycle Pathway antibodies  Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Release Cycle Pathway antibodies  Neurotransmitter Release Cycle Pathway Diagram
 Synaptic Vesicle Pathway antibodies  Synaptic Vesicle Pathway Diagram
 Synaptic Vesicle Cycle Pathway antibodies  Synaptic Vesicle Cycle Pathway Diagram
 Synaptic Vesicle Cycle Pathway antibodies  Synaptic Vesicle Cycle Pathway Diagram
 Transmission Across Chemical Synapses Pathway antibodies  Transmission Across Chemical Synapses Pathway Diagram
Diseases associated with VAChT elisa kitOrgans/Tissues associated with VAChT elisa kit
 Disease Name  Pubmed Publications
 Death Antibodies  >11 publications with VAChT and Death
 Cardiovascular Diseases Antibodies  >11 publications with VAChT and Cardiovascular Diseases
 Memory Disorders Antibodies  >10 publications with VAChT and Memory Disorders
 Cognition Disorders Antibodies  >9 publications with VAChT and Cognition Disorders
 Learning Disorders Antibodies  >9 publications with VAChT and Learning Disorders
 Inflammation Antibodies  >9 publications with VAChT and Inflammation
 Movement Disorders Antibodies  >7 publications with VAChT and Movement Disorders
 Congenital Abnormalities Antibodies  >5 publications with VAChT and Congenital Abnormalities
 Necrosis Antibodies  >4 publications with VAChT and Necrosis
 Immune System Diseases Antibodies  >3 publications with VAChT and Immune System Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >183 publications with VAChT and Brain
 Eye Antibodies  >21 publications with VAChT and Eye
 Liver Antibodies  >12 publications with VAChT and Liver
 Placenta Antibodies  >3 publications with VAChT and Placenta
 Testis Antibodies  >1 publications with VAChT and Testis
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