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RSAD2 elisa kit :: Human Virus Inhibitory Protein (Viperin) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023571 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $535 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,945 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Virus Inhibitory Protein (Viperin) (RSAD2), ELISA Kit

★Popular Item★
 Also Known As   

Virus Inhibitory Protein (Viperin) ELISA Kit

 Product Synonym Names    Cig5; RSAD2; vig1; Radical S-Adenosyl Methionine Domain Containing 2; Viperin,Endoplasmic Reticulum-Associated,Interferon-Inducible; Cytomegalovirus-induced gene 5
 Product Gene Name   

RSAD2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    607810
 3D Structure    ModBase 3D Structure for Q8WXG1
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Viperin. No significant cross-reactivity or interference between Viperin and analogues was observed.
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 Samples    Tissue homogenates, cell lysates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    <0.055ng/mL.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Viperin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Viperin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RSAD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RSAD2 purchase    MBS2023571 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Virus Inhibitory Protein (Viperin) (RSAD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing RSAD2, with an estimated sensitivity of <0.055ng/mL. The ELISA analytical biochemical technique of the MBS2023571 kit is based on RSAD2 antibody-RSAD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RSAD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RSAD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RSAD2 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Viperin in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Viperin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Viperin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Viperin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Viperin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for RSAD2 elisa kit    Signal transduction
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  Typical Testing Data/Standard Curve (for reference only) of RSAD2 elisa kit    RSAD2 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023571. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RSAD2. It may not necessarily be applicable to this product.
 NCBI GI #    17974550
 NCBI GeneID    91543
 NCBI Accession #    AAL50053.1 [Other Products]
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 UniProt Primary Accession #    Q8WXG1 [Other Products]
 UniProt Secondary Accession #    Q8WVI4 [Other Products]
 UniProt Related Accession #    Q8WXG1 [Other Products]
 Molecular Weight    42,170 Da [Similar Products]
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 NCBI Official Full Name    viperin
 NCBI Official Synonym Full Names    radical S-adenosyl methionine domain containing 2
 NCBI Official Symbol    RSAD2 [Similar Products]
 NCBI Official Synonym Symbols   
cig5; vig1; cig33; 2510004L01Rik
[Similar Products]
 NCBI Protein Information    radical S-adenosyl methionine domain-containing protein 2
 UniProt Protein Name    Radical S-adenosyl methionine domain-containing protein 2
 UniProt Synonym Protein Names   
Cytomegalovirus-induced gene 5 protein; Viperin; Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible
 Protein Family    Radical S-adenosyl methionine domain-containing protein
 UniProt Gene Name    RSAD2 [Similar Products]
 UniProt Entry Name    RSAD2_HUMAN
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 UniProt Comments for RSAD2    RSAD2: Interferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. Belongs to the radical SAM superfamily. RSAD2 family.

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; lipid particle

Molecular Function: protein binding; protein self-association; 4 iron, 4 sulfur cluster binding; metal ion binding; catalytic activity

Biological Process: positive regulation of toll-like receptor 7 signaling pathway; negative regulation of protein secretion; ossification; viral reproduction; negative regulation of viral genome replication; CD4-positive, alpha beta T cell differentiation; metabolic process; cytokine and chemokine mediated signaling pathway; response to virus; positive regulation of toll-like receptor 9 signaling pathway; regulation of ossification; defense response to virus
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 Research Articles on RSAD2    1. data suggested that viperin impaired respiratory syncytial virus (RSV) transmission by inhibiting virus filament formation, providing a basis for its anti-virus activity in RSV-infected cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RSAD2 elisa kitPathways associated with RSAD2 elisa kit
 Reference Product  PubMed Publications
 ISG15 elisa kit  >15 publications with RSAD2 and ISG15
 IFIT1 elisa kit  >9 publications with RSAD2 and IFIT1
 IRF7 elisa kit  >6 publications with RSAD2 and IRF7
 IFI44L elisa kit  >5 publications with RSAD2 and IFI44L
 IFIT2 elisa kit  >4 publications with RSAD2 and IFIT2
 IFIH1 elisa kit  >3 publications with RSAD2 and IFIH1
 IFIT3 elisa kit  >3 publications with RSAD2 and IFIT3
 CMPK2 elisa kit  >2 publications with RSAD2 and CMPK2
 RTP4 elisa kit  >1 publications with RSAD2 and RTP4
 XAF1 elisa kit  >1 publications with RSAD2 and XAF1
 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Interferon Alpha/beta Signaling Pathway antibodies  Interferon Alpha/beta Signaling Pathway Diagram
Diseases associated with RSAD2 elisa kitOrgans/Tissues associated with RSAD2 elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >11 publications with RSAD2 and Liver Diseases
 Nervous System Diseases Antibodies  >9 publications with RSAD2 and Nervous System Diseases
 Inflammation Antibodies  >7 publications with RSAD2 and Inflammation
 Influenza, Human Antibodies  >6 publications with RSAD2 and Influenza, Human
 Necrosis Antibodies  >4 publications with RSAD2 and Necrosis
 Carcinoma Antibodies  >4 publications with RSAD2 and Carcinoma
 Kidney Diseases Antibodies  >3 publications with RSAD2 and Kidney Diseases
 Atherosclerosis Antibodies  >3 publications with RSAD2 and Atherosclerosis
 Carcinoma, Hepatocellular Antibodies  >2 publications with RSAD2 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >2 publications with RSAD2 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >16 publications with RSAD2 and Blood
 Liver Antibodies  >10 publications with RSAD2 and Liver
 Uterus Antibodies  >6 publications with RSAD2 and Uterus
 Brain Antibodies  >4 publications with RSAD2 and Brain
 Connective Tissue Antibodies  >2 publications with RSAD2 and Connective Tissue
 Heart Antibodies  >2 publications with RSAD2 and Heart
 Eye Antibodies  >2 publications with RSAD2 and Eye
 Lung Antibodies  >2 publications with RSAD2 and Lung
 Pharynx Antibodies  >1 publications with RSAD2 and Pharynx
 Spleen Antibodies  >1 publications with RSAD2 and Spleen
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