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WISP2 elisa kit :: Human WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023164 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

WNT1 Inducible Signaling Pathway Protein 2 (WISP2), ELISA Kit

★Popular Item★
 Also Known As   

WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit

 Product Synonym Names    CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58
 Product Gene Name   

WISP2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603399
 3D Structure    ModBase 3D Structure for O76076
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of WISP2. No significant cross-reactivity or interference between WISP2 and analogues was observed.
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 Samples    Tissue homogenates, cell lysates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.312-20ng/mL
 Sensitivity    0.112ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WISP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WISP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of WISP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for WISP2 purchase    MBS2023164 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing WISP2, with an estimated sensitivity of 0.112ng/mL. The ELISA analytical biochemical technique of the MBS2023164 kit is based on WISP2 antibody-WISP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WISP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WISP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for WISP2 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WISP2 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to WISP2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WISP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WISP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of WISP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for WISP2 elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of WISP2 elisa kit    WISP2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023164. Click to request current manual
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NCBI/Uniprot data below describe general gene information for WISP2. It may not necessarily be applicable to this product.
 NCBI GI #    4507923
 NCBI GeneID    8839
 NCBI Accession #    NP_003872.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003881.2 [Other Products]
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 UniProt Primary Accession #    O76076 [Other Products]
 UniProt Secondary Accession #    Q6PEG3; B2R9N4; E1P612 [Other Products]
 UniProt Related Accession #    O76076 [Other Products]
 Molecular Weight    22,577 Da
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 NCBI Official Full Name    WNT1-inducible-signaling pathway protein 2
 NCBI Official Synonym Full Names    WNT1 inducible signaling pathway protein 2
 NCBI Official Symbol    WISP2 [Similar Products]
 NCBI Official Synonym Symbols   
CCN5; CT58; CTGF-L
[Similar Products]
 NCBI Protein Information    WNT1-inducible-signaling pathway protein 2
 UniProt Protein Name    WNT1-inducible-signaling pathway protein 2
 UniProt Synonym Protein Names   
CCN family member 5; Connective tissue growth factor-like protein; CTGF-L; Connective tissue growth factor-related protein 58
 Protein Family    WNT1-inducible-signaling pathway protein
 UniProt Gene Name    WISP2 [Similar Products]
 UniProt Synonym Gene Names    CCN5; CT58; CTGFL; WISP-2; CTGF-L [Similar Products]
 UniProt Entry Name    WISP2_HUMAN
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 NCBI Summary for WISP2    This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. [provided by RefSeq, Jul 2008]
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 UniProt Comments for WISP2    WISP2: May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production. Belongs to the CCN family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space; proteinaceous extracellular matrix; plasma membrane

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding

Biological Process: cell-cell signaling; regulation of cell growth; cell adhesion; signal transduction
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 Research Articles on WISP2    1. WISP2 has a role in regulating tumor cell susceptibility through EMT by inducing the TGF-beta signaling pathway, KLF-4 expression and miR-7 inhibition.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with WISP2 elisa kitDiseases associated with WISP2 elisa kit
 Reference Product  PubMed Publications
 IGF1 elisa kit  >2 publications with WISP2 and IGF1
 TGFB1 elisa kit  >2 publications with WISP2 and TGFB1
 HDAC1 elisa kit  >1 publications with WISP2 and HDAC1
 IGF2 elisa kit  >1 publications with WISP2 and IGF2
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >28 publications with WISP2 and Breast Neoplasms
 Fibrosis Antibodies  >8 publications with WISP2 and Fibrosis
 Inflammation Antibodies  >7 publications with WISP2 and Inflammation
 Neoplasm Invasiveness Antibodies  >7 publications with WISP2 and Neoplasm Invasiveness
 Cell Transformation, Neoplastic Antibodies  >6 publications with WISP2 and Cell Transformation, Neoplastic
 Hyperplasia Antibodies  >6 publications with WISP2 and Hyperplasia
 Liver Diseases Antibodies  >5 publications with WISP2 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with WISP2 and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >4 publications with WISP2 and Cardiovascular Diseases
 Liver Neoplasms Antibodies  >3 publications with WISP2 and Liver Neoplasms
Organs/Tissues associated with WISP2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >26 publications with WISP2 and Connective Tissue
 Bone Antibodies  >16 publications with WISP2 and Bone
 Muscle Antibodies  >14 publications with WISP2 and Muscle
 Liver Antibodies  >7 publications with WISP2 and Liver
 Blood Antibodies  >6 publications with WISP2 and Blood
 Lung Antibodies  >5 publications with WISP2 and Lung
 Uterus Antibodies  >3 publications with WISP2 and Uterus
 Pancreas Antibodies  >3 publications with WISP2 and Pancreas
 Lymph Node Antibodies  >2 publications with WISP2 and Lymph Node
 Ovary Antibodies  >2 publications with WISP2 and Ovary
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