Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow WNT3A rightarrow Human WNT3A  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • PPAR-delta ELISA Kit
 • Complement C3 Native Protein
 • MLN cDNA Clone
 • Chromogranin A-CHGA Antibody
 • B3GALTL Antibody
 • AMPD3 Antibody
 • Syntaxin 4 (STX4) Antibody
 • 5-LO ELISA Kit
 • PLACENTAL LACTOGEN (CSH1) Purified Protein
 • Histidine-rich glycoprotein (HRG) Recombinant Protein
 • Insulin Autoantibody (IAA) ELISA Kit
 • ED-B domain of fibronectin (EDB(-) FN) Antibody
 • SIRT7 Antibody
 • Bcl-10 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

WNT3A elisa kit :: Human Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2025504 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $535 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,945 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Wingless Type MMTV Integration Site Family, Member 3A (WNT3A), ELISA Kit

★Popular Item★
 Also Known As   

Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit

 Product Gene Name   

WNT3A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606359
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A).
No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and analogues was observed.
Section Bar
 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.055ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
Section Bar
 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of WNT3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for WNT3A purchase    MBS2025504 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing WNT3A, with an estimated sensitivity of < 0.055ng/mL. The ELISA analytical biochemical technique of the MBS2025504 kit is based on WNT3A antibody-WNT3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for WNT3A elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 3A (WNT3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for WNT3A elisa kit    Signal transduction
Section Bar
 Typical Testing Data/Standard Curve of WNT3A elisa kit    WNT3A elisa kit Typical Testing Data/Standard Curve image
Table BarTOPTable Bar
Sample Manual Insert of MBS2025504. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for WNT3A. It may not necessarily be applicable to this product.
 NCBI GI #    119590235
 NCBI GeneID    89780
 NCBI Accession #    EAW69829.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q3SY79; Q3SY80; Q969P2 [Other Products]
 UniProt Related Accession #    P56704 [Other Products]
 Molecular Weight    42,905 Da
Table BarTOPTable Bar
 NCBI Official Full Name    wingless-type MMTV integration site family, member 3A
 NCBI Official Synonym Full Names    wingless-type MMTV integration site family, member 3A
 NCBI Official Symbol    WNT3A [Similar Products]
 NCBI Protein Information    protein Wnt-3a
 UniProt Protein Name    Protein Wnt-3a
 UniProt Gene Name    WNT3A [Similar Products]
 UniProt Entry Name    WNT3A_HUMAN
Section Bar
 NCBI Summary for WNT3A    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for WNT3A    WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; early endosome membrane; Golgi lumen; endoplasmic reticulum lumen; extracellular region; plasma membrane

Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding

Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; cell proliferation in forebrain; positive regulation of receptor internalization; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; positive regulation of collateral sprouting in the absence of injury; palate development; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation
Table BarTOPTable Bar
 Research Articles on WNT3A    1. SNP rs752107 in WNT3A was strongly associated with decreased total hip BMD showing the highest significance under the recessive model
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with WNT3A elisa kitPathways associated with WNT3A elisa kit
 Reference Product  PubMed Publications
 CTNNB1 elisa kit  >788 publications with WNT3A and CTNNB1
 DKK1 elisa kit  >137 publications with WNT3A and DKK1
 LRP6 elisa kit  >105 publications with WNT3A and LRP6
 LRP5 elisa kit  >101 publications with WNT3A and LRP5
 FZD1 elisa kit  >21 publications with WNT3A and FZD1
 SFRP2 elisa kit  >17 publications with WNT3A and SFRP2
 DVL2 elisa kit  >17 publications with WNT3A and DVL2
 DVL3 elisa kit  >13 publications with WNT3A and DVL3
 FZD8 elisa kit  >8 publications with WNT3A and FZD8
 DVL1 elisa kit  >7 publications with WNT3A and DVL1
 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Canonical Wnt Signaling Pathway antibodies  Canonical Wnt Signaling Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Of Signal Transduction Pathway antibodies  Diseases Of Signal Transduction Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
Diseases associated with WNT3A elisa kitOrgans/Tissues associated with WNT3A elisa kit
 Disease Name  Pubmed Publications
 Death Antibodies  >52 publications with WNT3A and Death
 Breast Neoplasms Antibodies  >38 publications with WNT3A and Breast Neoplasms
 Embryo Loss Antibodies  >34 publications with WNT3A and Embryo Loss
 Necrosis Antibodies  >31 publications with WNT3A and Necrosis
 Inflammation Antibodies  >31 publications with WNT3A and Inflammation
 Fibrosis Antibodies  >27 publications with WNT3A and Fibrosis
 Liver Diseases Antibodies  >25 publications with WNT3A and Liver Diseases
 Liver Neoplasms Antibodies  >19 publications with WNT3A and Liver Neoplasms
 Brain Diseases Antibodies  >19 publications with WNT3A and Brain Diseases
 Neoplasms, Experimental Antibodies  >15 publications with WNT3A and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >58 publications with WNT3A and Connective Tissue
 Lung Antibodies  >42 publications with WNT3A and Lung
 Placenta Antibodies  >13 publications with WNT3A and Placenta
 Testis Antibodies  >6 publications with WNT3A and Testis
 Trachea Antibodies  >2 publications with WNT3A and Trachea
 Uncharacterized Tissue Antibodies  >1 publications with WNT3A and Uncharacterized Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions