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WNT5B elisa kit :: Human Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020878 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $535 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,945 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Wingless Type MMTV Integration Site Family, Member 5B (WNT5B), ELISA Kit

★Popular Item★
 Also Known As   

Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA Kit

 Product Gene Name   

WNT5B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606361
 3D Structure    ModBase 3D Structure for Q9H1J7
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B).
No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    1.56-100ng/mL
 Sensitivity    < 0.55ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of WNT5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for WNT5B purchase    MBS2020878 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56-100ng/mL in biological research samples containing WNT5B, with an estimated sensitivity of < 0.55ng/mL. The ELISA analytical biochemical technique of the MBS2020878 kit is based on WNT5B antibody-WNT5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for WNT5B elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 5B (WNT5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for WNT5B elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of WNT5B elisa kit    WNT5B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020878. Click to request current manual
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NCBI/Uniprot data below describe general gene information for WNT5B. It may not necessarily be applicable to this product.
 NCBI GI #    12804649
 NCBI GeneID    81029
 NCBI Accession #    AAH01749.1 [Other Products]
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 UniProt Primary Accession #    Q9H1J7 [Other Products]
 UniProt Secondary Accession #    Q96S49; Q9BV04; A8K315; D3DUP9 [Other Products]
 UniProt Related Accession #    Q9H1J7 [Other Products]
 Molecular Weight    40,323 Da
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 NCBI Official Full Name    Wingless-type MMTV integration site family, member 5B
 NCBI Official Synonym Full Names    wingless-type MMTV integration site family, member 5B
 NCBI Official Symbol    WNT5B [Similar Products]
 NCBI Protein Information    protein Wnt-5b
 UniProt Protein Name    Protein Wnt-5b
 UniProt Gene Name    WNT5B [Similar Products]
 UniProt Entry Name    WNT5B_HUMAN
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 NCBI Summary for WNT5B    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
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 UniProt Comments for WNT5B    WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region

Molecular Function: frizzled binding; receptor binding

Biological Process: fat cell differentiation; neuron differentiation; Wnt receptor signaling pathway; cell fate commitment; wound healing; positive regulation of fat cell differentiation; chondrocyte differentiation; positive regulation of cell migration
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 Research Articles on WNT5B    1. Report increased Wnt5b levels in adriamycin treated liver cancer stem cells within 4h of treatment.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with WNT5B elisa kitPathways associated with WNT5B elisa kit
 Reference Product  PubMed Publications
 FZD1 elisa kit  >10 publications with WNT5B and FZD1
 FZD6 elisa kit  >10 publications with WNT5B and FZD6
 FZD7 elisa kit  >10 publications with WNT5B and FZD7
 FZD4 elisa kit  >9 publications with WNT5B and FZD4
 FZD5 elisa kit  >8 publications with WNT5B and FZD5
 FZD10 elisa kit  >5 publications with WNT5B and FZD10
 SFRP2 elisa kit  >4 publications with WNT5B and SFRP2
 FZD8 elisa kit  >3 publications with WNT5B and FZD8
 SFRP5 elisa kit  >2 publications with WNT5B and SFRP5
 PORCN elisa kit  >1 publications with WNT5B and PORCN
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
Diseases associated with WNT5B elisa kitOrgans/Tissues associated with WNT5B elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6 publications with WNT5B and Inflammation
 Lung Neoplasms Antibodies  >5 publications with WNT5B and Lung Neoplasms
 Disease Models, Animal Antibodies  >5 publications with WNT5B and Disease Models, Animal
 Necrosis Antibodies  >4 publications with WNT5B and Necrosis
 Kidney Diseases Antibodies  >4 publications with WNT5B and Kidney Diseases
 Nervous System Diseases Antibodies  >4 publications with WNT5B and Nervous System Diseases
 Fibrosis Antibodies  >4 publications with WNT5B and Fibrosis
 Lung Diseases Antibodies  >3 publications with WNT5B and Lung Diseases
 Liver Diseases Antibodies  >2 publications with WNT5B and Liver Diseases
 Hypertrophy Antibodies  >2 publications with WNT5B and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >21 publications with WNT5B and Embryonic Tissue
 Bone Antibodies  >20 publications with WNT5B and Bone
 Muscle Antibodies  >14 publications with WNT5B and Muscle
 Brain Antibodies  >12 publications with WNT5B and Brain
 Lung Antibodies  >11 publications with WNT5B and Lung
 Kidney Antibodies  >10 publications with WNT5B and Kidney
 Liver Antibodies  >8 publications with WNT5B and Liver
 Eye Antibodies  >7 publications with WNT5B and Eye
 Heart Antibodies  >7 publications with WNT5B and Heart
 Intestine Antibodies  >6 publications with WNT5B and Intestine
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