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PEPD elisa kit :: Human Xaa-Pro dipeptidase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2883088
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $560 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,445 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 
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 Product Name   

Xaa-Pro dipeptidase (PEPD), ELISA Kit

★Popular Item★
 Also Known As   

Human Xaa-Pro dipeptidase ELISA Kit

 Product Synonym Names    Xaa-Pro dipeptidase; X-Pro dipeptidase; Imidodipeptidase; Peptidase D; Proline dipeptidase; Prolidase; PEPD; PRD; 3.4.13.9
 Product Gene Name   

PEPD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2883088 Testing Data    Testing Data PDF
 OMIM    170100
 3D Structure    ModBase 3D Structure for P12955
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 Species Reactivity    Human
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 Specificity    This assay recognizes recombinant and natural human PEPD. No significant cross-reactivity or interference was observed.
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 Samples    Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
 Detection Range    0.78 ng/mL -50.0 ng/mL.
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PEPD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PEPD purchase    MBS2883088 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Xaa-Pro dipeptidase (PEPD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78 ng/mL -50.0 ng/mL in biological research samples containing PEPD. The ELISA analytical biochemical technique of the MBS2883088 kit is based on PEPD antibody-PEPD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PEPD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PEPD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PEPD elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human xaa-pro dipeptidase,PEPD concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PEPD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PEPD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PEPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PEPD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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  Typical Testing Data/Standard Curve (for reference only) of PEPD elisa kit    PEPD elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2883088. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PEPD. It may not necessarily be applicable to this product.
 NCBI GI #    149589008
 NCBI GeneID    5184
 NCBI Accession #    NP_000276.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000285.3 [Other Products]
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 UniProt Primary Accession #    P12955 [Other Products]
 UniProt Secondary Accession #    Q8TBN9; Q9BT75; A8K3Z1; A8K416; A8K696; A8MX47; B4DDB7; B4DGJ1; E9PCE8 [Other Products]
 UniProt Related Accession #    P12955 [Other Products]
 Molecular Weight    47,261 Da
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 NCBI Official Full Name    xaa-Pro dipeptidase isoform 1
 NCBI Official Synonym Full Names    peptidase D
 NCBI Official Symbol    PEPD [Similar Products]
 NCBI Official Synonym Symbols   
PROLIDASE
[Similar Products]
 NCBI Protein Information    xaa-Pro dipeptidase
 UniProt Protein Name    Xaa-Pro dipeptidase
 UniProt Synonym Protein Names   
Imidodipeptidase; Peptidase D; Proline dipeptidase; Prolidase
 Protein Family    Xaa-Pro dipeptidase
 UniProt Gene Name    PEPD [Similar Products]
 UniProt Synonym Gene Names    PRD; X-Pro dipeptidase; Prolidase [Similar Products]
 UniProt Entry Name    PEPD_HUMAN
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 NCBI Summary for PEPD    This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
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 UniProt Comments for PEPD    peptidase D: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait. Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.13.9

Chromosomal Location of Human Ortholog: 19q13.11

Molecular Function: aminopeptidase activity; dipeptidase activity; manganese ion binding; metallocarboxypeptidase activity; protein binding

Biological Process: amino acid metabolic process; collagen catabolic process; proteolysis

Disease: Prolidase Deficiency
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 Research Articles on PEPD    1. Serum prolidase level was significantly higher in schizophrenia patients compared to the controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PEPD elisa kitDiseases associated with PEPD elisa kit
 Reference Product  PubMed Publications
 APOC1 elisa kit  >2 publications with PEPD and APOC1
 ERBB2 elisa kit  >2 publications with PEPD and ERBB2
 PTGS2 elisa kit  >1 publications with PEPD and PTGS2
 GSR elisa kit  >1 publications with PEPD and GSR
 Disease Name  Pubmed Publications
 Prolidase Deficiency Antibodies  >22 publications with PEPD and Prolidase Deficiency
 Vascular Diseases Antibodies  >10 publications with PEPD and Vascular Diseases
 Infection Antibodies  >8 publications with PEPD and Infection
 Splenomegaly Antibodies  >5 publications with PEPD and Splenomegaly
 Respiratory Tract Diseases Antibodies  >4 publications with PEPD and Respiratory Tract Diseases
 Abnormalities, Multiple Antibodies  >4 publications with PEPD and Abnormalities, Multiple
 Hypertrophy Antibodies  >3 publications with PEPD and Hypertrophy
 Skin Ulcer Antibodies  >3 publications with PEPD and Skin Ulcer
 Diabetes Mellitus, Type 2 Antibodies  >3 publications with PEPD and Diabetes Mellitus, Type 2
 Liver Diseases Antibodies  >2 publications with PEPD and Liver Diseases
Organs/Tissues associated with PEPD elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >18 publications with PEPD and Skin
 Blood Antibodies  >18 publications with PEPD and Blood
 Ganglia Antibodies  >8 publications with PEPD and Ganglia
 Muscle Antibodies  >6 publications with PEPD and Muscle
 Kidney Antibodies  >5 publications with PEPD and Kidney
 Intestine Antibodies  >4 publications with PEPD and Intestine
 Lung Antibodies  >3 publications with PEPD and Lung
 Vascular Antibodies  >3 publications with PEPD and Vascular
 Heart Antibodies  >2 publications with PEPD and Heart
 Nerve Antibodies  >2 publications with PEPD and Nerve
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