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YBX1 elisa kit :: Human Y-Box Binding Protein 1 (YBX1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS456117
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $400 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,425 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,710 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Y-Box Binding Protein 1 (YBX1), ELISA Kit

★Popular Item★
 Also Known As   

Human Y-Box Binding Protein 1 (YBX1) ELISA Kit

 Product Gene Name   

YBX1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    M24070 mRNA
 3D Structure    ModBase 3D Structure for P67809
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of YBX1. No significant cross-reactivity or interference between YBX1 and analogues was observed.
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 Samples    Tissue homogenates, cell lysates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.063ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level YBX1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level YBX1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of YBX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for YBX1 purchase    MBS456117 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Y-Box Binding Protein 1 (YBX1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing YBX1, with an estimated sensitivity of 0.063ng/mL. The ELISA analytical biochemical technique of the MBS456117 kit is based on YBX1 antibody-YBX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YBX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YBX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for YBX1 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of YBX1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to YBX1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to YBX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain YBX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of YBX1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of YBX1 elisa kit    YBX1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS456117. Click to request current manual
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NCBI/Uniprot data below describe general gene information for YBX1. It may not necessarily be applicable to this product.
 NCBI GI #    34098946
 NCBI GeneID    4904
 NCBI Accession #    NP_004550.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004559.4 [Other Products]
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 UniProt Primary Accession #    P67809 [Other Products]
 UniProt Secondary Accession #    P16990; P16991; Q14972; Q15325; Q5FVF0 [Other Products]
 UniProt Related Accession #    P67809 [Other Products]
 Molecular Weight    35,924 Da
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 NCBI Official Full Name    nuclease-sensitive element-binding protein 1
 NCBI Official Synonym Full Names    Y-box binding protein 1
 NCBI Official Symbol    YBX1 [Similar Products]
 NCBI Official Synonym Symbols   
YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1
[Similar Products]
 NCBI Protein Information    nuclease-sensitive element-binding protein 1
 UniProt Protein Name    Nuclease-sensitive element-binding protein 1
 UniProt Synonym Protein Names   
CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1
 Protein Family    Nuclease-sensitive element-binding protein
 UniProt Gene Name    YBX1 [Similar Products]
 UniProt Synonym Gene Names    NSEP1; YB1; CBF-A; DBPB; EFI-A; YB-1 [Similar Products]
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 NCBI Summary for YBX1    This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]
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 UniProt Comments for YBX1    Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro.
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 Research Articles on YBX1    1. Study shows that DeltaNp63alpha controls YB-1 protein stability suggesting that DeltaNp63alpha/YB-1 cross talk is relevant for survival of basal keratinocytes in stratified epithelia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with YBX1 elisa kitPathways associated with YBX1 elisa kit
 Reference Product  PubMed Publications
 TP53 elisa kit  >3 publications with YBX1 and TP53
 DHX9 elisa kit  >2 publications with YBX1 and DHX9
 HNRNPA2B1 elisa kit  >2 publications with YBX1 and HNRNPA2B1
 HNRNPD elisa kit  >2 publications with YBX1 and HNRNPD
 UBC elisa kit  >2 publications with YBX1 and UBC
 HNRNPU elisa kit  >1 publications with YBX1 and HNRNPU
 PTPN1 elisa kit  >1 publications with YBX1 and PTPN1
 Products by Pathway  Pathway Diagram
 Brain-Derived Neurotrophic Factor (BDNF) Signaling Pathway antibodies  Brain-Derived Neurotrophic Factor (BDNF) Signaling Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 MECP2 And Associated Rett Syndrome Pathway antibodies  MECP2 And Associated Rett Syndrome Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Signaling Events Mediated By PTP1B Pathway antibodies  Signaling Events Mediated By PTP1B Pathway Diagram
 Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways antibodies  Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
 MRNA Splicing - Minor Pathway antibodies  MRNA Splicing - Minor Pathway Diagram
Diseases associated with YBX1 elisa kitOrgans/Tissues associated with YBX1 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >57 publications with YBX1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >23 publications with YBX1 and Neoplasms, Experimental
 Liver Diseases Antibodies  >18 publications with YBX1 and Liver Diseases
 Lung Diseases Antibodies  >17 publications with YBX1 and Lung Diseases
 Immune System Diseases Antibodies  >13 publications with YBX1 and Immune System Diseases
 Liver Neoplasms Antibodies  >13 publications with YBX1 and Liver Neoplasms
 Disease Models, Animal Antibodies  >11 publications with YBX1 and Disease Models, Animal
 Kidney Diseases Antibodies  >11 publications with YBX1 and Kidney Diseases
 Carcinoma, Squamous Cell Antibodies  >11 publications with YBX1 and Carcinoma, Squamous Cell
 Carcinoma, Hepatocellular Antibodies  >10 publications with YBX1 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >88 publications with YBX1 and Liver
 Brain Antibodies  >42 publications with YBX1 and Brain
 Lung Antibodies  >41 publications with YBX1 and Lung
 Blood Antibodies  >37 publications with YBX1 and Blood
 Kidney Antibodies  >35 publications with YBX1 and Kidney
 Prostate Antibodies  >21 publications with YBX1 and Prostate
 Muscle Antibodies  >20 publications with YBX1 and Muscle
 Vascular Antibodies  >18 publications with YBX1 and Vascular
 Skin Antibodies  >14 publications with YBX1 and Skin
 Thyroid Antibodies  >13 publications with YBX1 and Thyroid
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