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ZBP1 elisa kit :: Human Z DNA binding protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS732908
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Z DNA binding protein (ZBP1), ELISA Kit

 Also Known As   

Human Z DNA binding protein ELISA Kit

 Product Gene Name   

ZBP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606750
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 Species Reactivity    Human
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    250-5000pg/mL
 Sensitivity    1.0 pg/mL
 Highest Standards    5000pg/mL
 Concentration of Standards    Concentration of Standards
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ZBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ZBP1 purchase    MBS732908 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Z DNA binding protein (ZBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 250-5000pg/mL in biological research samples containing ZBP1, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS732908 kit is based on ZBP1 antibody-ZBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ZBP1 elisa kit

   For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Intended Uses: This ZBP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ZBP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ZBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZBP1 antibody and an ZBP1-HRP conjugate. The assay sample and buffer are incubated together with ZBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZBP1 concentration since ZBP1 from samples and ZBP1-HRP conjugate compete for the anti-ZBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ZBP1 from the sample, fewer sites are left to bind ZBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZBP1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ZBP1 elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of ZBP1 elisa kit    ZBP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS732908. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ZBP1. It may not necessarily be applicable to this product.
 NCBI GI #    238550212
 NCBI GeneID    81030
 NCBI Accession #    NP_001153891.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001160419.2 [Other Products]
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 UniProt Secondary Accession #    Q5JY39; Q9BYW4; A2A2F7; B3KVA1; F5GYT1 [Other Products]
 UniProt Related Accession #    Q9H171 [Other Products]
 Molecular Weight    38,220 Da
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 NCBI Official Full Name    Z-DNA-binding protein 1 isoform d
 NCBI Official Synonym Full Names    Z-DNA binding protein 1
 NCBI Official Symbol    ZBP1 [Similar Products]
 NCBI Official Synonym Symbols   
DAI; DLM1; DLM-1; C20orf183
[Similar Products]
 NCBI Protein Information    Z-DNA-binding protein 1; DNA-dependent activator of IRFs; DNA-dependent activator of IFN-regulatory factors; tumor stroma and activated macrophage protein DLM-1; DNA-dependent activator of interferon regulatory factors
 UniProt Protein Name    Z-DNA-binding protein 1
 UniProt Synonym Protein Names   
Tumor stroma and activated macrophage protein DLM-1
 Protein Family    Z-DNA-binding protein
 UniProt Gene Name    ZBP1 [Similar Products]
 UniProt Synonym Gene Names    C20orf183; DLM1 [Similar Products]
 UniProt Entry Name    ZBP1_HUMAN
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 NCBI Summary for ZBP1    This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
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 UniProt Comments for ZBP1    ZBP1: Participates in the detection by the host's innate immune system of DNA from viral, bacterial or even host origin. Plays a role in host defense against tumors and pathogens. Acts as a cytoplasmic DNA sensor which, when activated, induces the recruitment of TBK1 and IRF3 to its C-terminal region and activates the downstream interferon regulatory factor (IRF) and NF-kappa B transcription factors, leading to type-I interferon production. ZBP1-induced NF-kappaB activation probably involves the recruitment of the RHIM containing kinases RIPK1 and RIPK3. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; left-handed Z-DNA binding; RNA binding

Biological Process: metabolic process; positive regulation of interferon type I production; innate immune response
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 Research Articles on ZBP1    1. Our results show that HSV2 is detected by a plethora of PRRs including DAI protein which trigger cytokine secretion to protect the host.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ZBP1 elisa kitPathways associated with ZBP1 elisa kit
 Reference Product  PubMed Publications
 IRF3 elisa kit  >15 publications with ZBP1 and IRF3
 TBK1 elisa kit  >7 publications with ZBP1 and TBK1
 RIPK3 elisa kit  >5 publications with ZBP1 and RIPK3
 IRF7 elisa kit  >4 publications with ZBP1 and IRF7
 TLR3 elisa kit  >3 publications with ZBP1 and TLR3
 RIPK1 elisa kit  >1 publications with ZBP1 and RIPK1
 Products by Pathway  Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 IRF3 Mediated Activation Of Type 1 IFN Pathway antibodies  IRF3 Mediated Activation Of Type 1 IFN Pathway Diagram
 IRF3-mediated Induction Of Type I IFN Pathway antibodies  IRF3-mediated Induction Of Type I IFN Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 RIP-mediated NFkB Activation Via ZBP1 Pathway antibodies  RIP-mediated NFkB Activation Via ZBP1 Pathway Diagram
 Regulation Of Innate Immune Responses To Cytosolic DNA Pathway antibodies  Regulation Of Innate Immune Responses To Cytosolic DNA Pathway Diagram
 STING Mediated Induction Of Host Immune Responses Pathway antibodies  STING Mediated Induction Of Host Immune Responses Pathway Diagram
Diseases associated with ZBP1 elisa kitOrgans/Tissues associated with ZBP1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >11 publications with ZBP1 and Inflammation
 Carcinoma Antibodies  >6 publications with ZBP1 and Carcinoma
 Necrosis Antibodies  >6 publications with ZBP1 and Necrosis
 Neoplasms, Experimental Antibodies  >5 publications with ZBP1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >4 publications with ZBP1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >3 publications with ZBP1 and Adenocarcinoma
 Kidney Diseases Antibodies  >3 publications with ZBP1 and Kidney Diseases
 Carcinoma, Hepatocellular Antibodies  >3 publications with ZBP1 and Carcinoma, Hepatocellular
 Disease Models, Animal Antibodies  >3 publications with ZBP1 and Disease Models, Animal
 Liver Neoplasms Antibodies  >2 publications with ZBP1 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >10 publications with ZBP1 and Blood
 Liver Antibodies  >5 publications with ZBP1 and Liver
 Lymph Node Antibodies  >3 publications with ZBP1 and Lymph Node
 Connective Tissue Antibodies  >1 publications with ZBP1 and Connective Tissue
 Spleen Antibodies  >1 publications with ZBP1 and Spleen
 Muscle Antibodies  >1 publications with ZBP1 and Muscle
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