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ACHE elisa kit :: Human acetylcholinesterase (Yt blood group) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704993
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

acetylcholinesterase (Yt blood group) (ACHE), ELISA Kit

 Also Known As   

Human Acetylcholinesterase, AChE ELISA Kit

 Product Synonym Names    Human Acetylcholinesterase; AChE ELISA Kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; acetylcholinesterase (Yt blood group)
 Product Gene Name   

ACHE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704993 Testing Data    Testing Data PDF
 OMIM    gene+112100
 3D Structure    ModBase 3D Structure for P22303
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ACHE. No significant cross-reactivity or interference between Human ACHE and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.94 mU/ml-60 mU/ml
 Sensitivity    0.24 mU/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Neurobiology
 Protein Biological Process 3    Neurotransmitter degradation
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACHE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACHE purchase    MBS704993 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the acetylcholinesterase (Yt blood group) (ACHE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.94 mU/ml-60 mU/ml in biological research samples containing ACHE, with an estimated sensitivity of 0.24 mU/ml. The ELISA analytical biochemical technique of the MBS704993 kit is based on ACHE antibody-ACHE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACHE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACHE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ACHE elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AChE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AChE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AChE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AChE bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ACHE elisa kit    ACHE elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704993. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ACHE. It may not necessarily be applicable to this product.
 NCBI GI #    4557239
 NCBI GeneID    43
 NCBI Accession #    NP_000656.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000665.3 [Other Products]
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 UniProt Primary Accession #    P22303 [Other Products]
 UniProt Secondary Accession #    Q16169; Q29S23; Q2M324; Q504V3; Q53F46; Q86TM9; Q86YX9; Q9BXP7; A4D2E2; B7ZKZ0; D6W5X7 [Other Products]
 UniProt Related Accession #    P22303 [Other Products]
 Molecular Weight    67,796 Da
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 NCBI Official Full Name    acetylcholinesterase isoform E4-E6
 NCBI Official Synonym Full Names    acetylcholinesterase (Yt blood group)
 NCBI Official Symbol    ACHE [Similar Products]
 NCBI Official Synonym Symbols   
YT; ACEE; ARACHE; N-ACHE
[Similar Products]
 NCBI Protein Information    acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase
 UniProt Protein Name    Acetylcholinesterase
 UniProt Gene Name    ACHE [Similar Products]
 UniProt Synonym Gene Names    AChE [Similar Products]
 UniProt Entry Name    ACES_HUMAN
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 NCBI Summary for ACHE    Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ACHE    Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.4 PublicationsManual assertion based on experiment in:Ref.11

Catalytic activity: Acetylcholine + H2O = choline + acetate.
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 Research Articles on ACHE    1. Synaptic acetylcholinesterase may be a tumor suppressor and is modulated by miR-212 in non-small cell lung cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ACHE elisa kitPathways associated with ACHE elisa kit
 Reference Product  PubMed Publications
 APP elisa kit  >121 publications with ACHE and APP
 COLQ elisa kit  >69 publications with ACHE and COLQ
 PRIMA1 elisa kit  >13 publications with ACHE and PRIMA1
 LAMB1 elisa kit  >1 publications with ACHE and LAMB1
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
Diseases associated with ACHE elisa kitOrgans/Tissues associated with ACHE elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >230881 publications with ACHE and Nervous System Diseases
 Heart Diseases Antibodies  >65755 publications with ACHE and Heart Diseases
 Brain Diseases Antibodies  >29632 publications with ACHE and Brain Diseases
 Inflammation Antibodies  >23034 publications with ACHE and Inflammation
 Necrosis Antibodies  >12315 publications with ACHE and Necrosis
 Hypertension Antibodies  >11393 publications with ACHE and Hypertension
 Kidney Diseases Antibodies  >10709 publications with ACHE and Kidney Diseases
 Edema Antibodies  >10539 publications with ACHE and Edema
 Liver Diseases Antibodies  >8740 publications with ACHE and Liver Diseases
 Weight Loss Antibodies  >6887 publications with ACHE and Weight Loss
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >67350 publications with ACHE and Blood
 Nerve Antibodies  >38396 publications with ACHE and Nerve
 Muscle Antibodies  >34991 publications with ACHE and Muscle
 Heart Antibodies  >34035 publications with ACHE and Heart
 Brain Antibodies  >28854 publications with ACHE and Brain
 Liver Antibodies  >11294 publications with ACHE and Liver
 Kidney Antibodies  >11032 publications with ACHE and Kidney
 Intestine Antibodies  >10435 publications with ACHE and Intestine
 Lung Antibodies  >9267 publications with ACHE and Lung
 Mouth Antibodies  >8595 publications with ACHE and Mouth
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