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AGXT elisa kit :: Human alanine-glyoxylate aminotransferase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS934004
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alanine-glyoxylate aminotransferase (AGXT), ELISA Kit

★Popular Item★
 Also Known As   

Human Serine--pyruvate aminotransferase, AGXT ELISA Kit

 Product Synonym Names    Human Serine--pyruvate aminotransferase (AGXT) ELISA kit; AGT; AGT1; AGXT1; PH1; SPAT; SPT; TLH6; L-alanine: glyoxylate aminotransferase 1; hepatic peroxisomal alanine:glyoxylate aminotransferase; serine-pyruvate aminotransferase; serine:pyruvate aminotransfe; alanine-glyoxylate aminotransferase
 Product Gene Name   

AGXT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS934004 Testing Data    Testing Data PDF
 OMIM    604285
 3D Structure    ModBase 3D Structure for P21549
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human AGXT. No significant cross-reactivity or interference between human AGXT and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    31.25 pg/ml-2000 pg/ml.
 Sensitivity    7.81 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AGXT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AGXT purchase    MBS934004 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alanine-glyoxylate aminotransferase (AGXT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing AGXT, with an estimated sensitivity of 7.81 pg/ml. The ELISA analytical biochemical technique of the MBS934004 kit is based on AGXT antibody-AGXT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGXT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGXT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AGXT elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AGXT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGXT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGXT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGXT bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of AGXT elisa kit    AGXT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS934004. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AGXT. It may not necessarily be applicable to this product.
 NCBI GI #    4557289
 NCBI GeneID    189
 NCBI Accession #    NP_000021.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000030.2 [Other Products]
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 UniProt Primary Accession #    P21549 [Other Products]
 UniProt Secondary Accession #    Q53QU6 [Other Products]
 UniProt Related Accession #    P21549 [Other Products]
 Molecular Weight    43,010 Da
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 NCBI Official Full Name    serine--pyruvate aminotransferase
 NCBI Official Synonym Full Names    alanine-glyoxylate aminotransferase
 NCBI Official Symbol    AGXT [Similar Products]
 NCBI Official Synonym Symbols   
AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1
[Similar Products]
 NCBI Protein Information    serine--pyruvate aminotransferase; serine-pyruvate aminotransferase; serine:pyruvate aminotransferase; alanine--glyoxylate aminotransferase; L-alanine: glyoxylate aminotransferase 1; hepatic peroxisomal alanine:glyoxylate aminotransferase
 UniProt Protein Name    Serine--pyruvate aminotransferase
 UniProt Synonym Protein Names   
Alanine--glyoxylate aminotransferase (EC:2.6.1.44); AGT
 Protein Family    Serine--pyruvate aminotransferase
 UniProt Gene Name    AGXT [Similar Products]
 UniProt Synonym Gene Names    AGT1; SPAT; SPT; AGT [Similar Products]
 UniProt Entry Name    SPYA_HUMAN
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 NCBI Summary for AGXT    This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AGXT    AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Motility/polarity/chemotaxis; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Mitochondrial; Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.51; EC 2.6.1.44

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: peroxisomal matrix; mitochondrial matrix; peroxisome

Molecular Function: amino acid binding; protein binding; protein homodimerization activity; alanine-glyoxylate transaminase activity; serine-pyruvate transaminase activity; transaminase activity; pyridoxal phosphate binding; receptor binding

Biological Process: pyruvate biosynthetic process; response to cAMP; glyoxylate catabolic process; glyoxylate metabolic process; glycine biosynthetic process, by transamination of glyoxylate; response to glucocorticoid stimulus; oxalic acid secretion; L-cysteine catabolic process; L-alanine catabolic process

Disease: Hyperoxaluria, Primary, Type I
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 Research Articles on AGXT    1. data imply that the AGT Pro11Leu polymorphism is not directly responsible for the low incidence of stone formation in black South Africans.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with AGXT elisa kitDiseases associated with AGXT elisa kit
 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glyoxylate And Dicarboxylate Metabolism Pathway antibodies  Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
 Glyoxylate And Dicarboxylate Metabolism Pathway antibodies  Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
 Glyoxylate Metabolism Pathway antibodies  Glyoxylate Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >62 publications with AGXT and Kidney Diseases
 Primary hyperoxaluria type 1 Antibodies  >50 publications with AGXT and Primary hyperoxaluria type 1
 Liver Diseases Antibodies  >6 publications with AGXT and Liver Diseases
 Disease Models, Animal Antibodies  >5 publications with AGXT and Disease Models, Animal
 Neoplasms Antibodies  >3 publications with AGXT and Neoplasms
 Drug Toxicity Antibodies  >2 publications with AGXT and Drug Toxicity
 Hypertension Antibodies  >2 publications with AGXT and Hypertension
 Necrosis Antibodies  >1 publications with AGXT and Necrosis
 Pancreatic Neoplasms Antibodies  >1 publications with AGXT and Pancreatic Neoplasms
 Congenital Abnormalities Antibodies  >1 publications with AGXT and Congenital Abnormalities
Organs/Tissues associated with AGXT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >39 publications with AGXT and Liver
 Kidney Antibodies  >20 publications with AGXT and Kidney
 Blood Antibodies  >9 publications with AGXT and Blood
 Intestine Antibodies  >1 publications with AGXT and Intestine
 Brain Antibodies  >1 publications with AGXT and Brain
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