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ADH1A elisa kit :: Human alcohol dehydrogenase 1A (class I) , alpha polypeptide ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700990
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

alcohol dehydrogenase 1A (class I) , alpha polypeptide (ADH1A), ELISA Kit

 Also Known As   

Human alcohol dehydrogenase, ADH ELISA Kit

 Product Synonym Names    Human alcohol dehydrogenase; ADH ELISA Kit; ADH1; ADH; alpha subunit; alcohol dehydrogenase 1 (class I); alpha polypeptide; aldehyde reductase; class I alcohol dehydrogenase; alpha subunit; alcohol dehydrogenase 1A (class I); alpha polypeptide
 Product Gene Name   

ADH1A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700990 Testing Data    Testing Data PDF
 OMIM    103700
 3D Structure    ModBase 3D Structure for P07327
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ADH1A. No significant cross-reactivity or interference between Human ADH1A and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates
 Detection Range    0.312 mU/ml-20 mU/ml
 Sensitivity    0.078 mU/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADH1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADH1A purchase    MBS700990 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alcohol dehydrogenase 1A (class I), alpha polypeptide (ADH1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 mU/ml-20 mU/ml in biological research samples containing ADH1A, with an estimated sensitivity of 0.078 mU/ml. The ELISA analytical biochemical technique of the MBS700990 kit is based on ADH1A antibody-ADH1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADH1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADH1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADH1A elisa kit

   Introduction: Alcohol dehydrogenase (ADH) is an enzyme discovered in the mid-1960s in Drosophila melanogaster. Since then, there has been extensive research on the enzyme. Alcohol dehydrogenase is a dimer, weighing 80 kDa. Alcohol dehydrogenases are a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. In humans, alcohol is metabolized by a rate of 1 ounce or 7 to 10 g/hour. Alcohol dehydrogenase is responsible for catalyzing oxidation of primary and secondary alcohols to aldehydes and ketones, respectively, and also can effect the reverse reaction. It does not work well with primary alcohols. Instead, it works the best with secondary and cyclic alcohols. In humans, it exists in multiple forms as a dimer and is encoded by at least seven different genes. There are five classes (I-V) of alcohol dehydrogenase, but the hepatic form that is primarily used in 3 humans is class 1. Class 1 consists of A,B, and C subunits that are encoded by the genes ADH1A, ADH1B, and ADH1C. The enzyme is contained in the lining of the stomach and in the liver. It catalyzes the oxidation of ethanol to acetaldehyde. This allows the consumption of alcoholic beverages, but its evolutionary purpose is probably the breakdown of alcohols naturally contained in foods or produced by bacteria in the digestive tract. Alcohol dehydrogenase is also involved in the toxicity of other types of alcohol: for instance, it oxidizes methanol to produce formaldehyde and ethylene glycol to ultimately yield glycolic and oxalic acids. Humans have at least six slightly different alcohol dehydrogenases. All of them are dimers (consist of two polypeptides), with each dimer containing two zinc ions Zn2+. One of those ions is crucial for the operation of the enzyme: it is located at the catalytic site and holds the hydroxyl group of the alcohol in place.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADH and Avidin conjugated to 4 Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ADH1A elisa kit    ADH1A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700990. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADH1A. It may not necessarily be applicable to this product.
 NCBI GI #    4501929
 NCBI GeneID    124
 NCBI Accession #    NP_000658.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000667.3 [Other Products]
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 UniProt Primary Accession #    P07327 [Other Products]
 UniProt Secondary Accession #    Q17R68; A8K3E3 [Other Products]
 UniProt Related Accession #    P07327 [Other Products]
 Molecular Weight    39,859 Da
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 NCBI Official Full Name    alcohol dehydrogenase 1A
 NCBI Official Synonym Full Names    alcohol dehydrogenase 1A (class I), alpha polypeptide
 NCBI Official Symbol    ADH1A [Similar Products]
 NCBI Official Synonym Symbols   
ADH1
[Similar Products]
 NCBI Protein Information    alcohol dehydrogenase 1A; ADH, alpha subunit; aldehyde reductase; alcohol dehydrogenase subunit alpha; alcohol dehydrogenase 1 (class I), alpha polypeptide
 UniProt Protein Name    Alcohol dehydrogenase 1A
 UniProt Synonym Protein Names   
Alcohol dehydrogenase subunit alpha
 UniProt Gene Name    ADH1A [Similar Products]
 UniProt Synonym Gene Names    ADH1 [Similar Products]
 UniProt Entry Name    ADH1A_HUMAN
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 NCBI Summary for ADH1A    This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]
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 UniProt Comments for ADH1A    ADH1A: a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase; Transcription regulation; Xenobiotic Metabolism - metabolism by cytochrome P450; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.1.1.1; Lipid Metabolism - fatty acid; Cofactor and Vitamin Metabolism - retinol; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: protein binding; zinc ion binding; alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent

Biological Process: xenobiotic metabolic process; alcohol metabolic process; ethanol oxidation; drug metabolic process
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 Research Articles on ADH1A    1. Unde-rexpression of the ADH1 gene, which influences the transformation of the extracellular matrix, plays a probable role in the etiology of uterine fibroid.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADH1A elisa kitPathways associated with ADH1A elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with ADH1A and FLNA
 Products by Pathway  Pathway Diagram
 Abacavir Metabolism Pathway antibodies  Abacavir Metabolism Pathway Diagram
 Abacavir Transport And Metabolism Pathway antibodies  Abacavir Transport And Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Ethanol Oxidation Pathway antibodies  Ethanol Oxidation Pathway Diagram
 Fatty Acid Omega Oxidation Pathway antibodies  Fatty Acid Omega Oxidation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
Diseases associated with ADH1A elisa kitOrgans/Tissues associated with ADH1A elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >3 publications with ADH1A and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with ADH1A and Carcinoma, Hepatocellular
 Ovarian Neoplasms Antibodies  >1 publications with ADH1A and Ovarian Neoplasms
 Recurrence Antibodies  >1 publications with ADH1A and Recurrence
 Necrosis Antibodies  >1 publications with ADH1A and Necrosis
 Poisoning Antibodies  >1 publications with ADH1A and Poisoning
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >742 publications with ADH1A and Brain
 Head Antibodies  >170 publications with ADH1A and Head
 Whole Body Antibodies  >26 publications with ADH1A and Whole Body
 Uncharacterized Tissue Antibodies  >2 publications with ADH1A and Uncharacterized Tissue
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