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A2ML1 elisa kit :: Human alpha-2-macroglobulin-like 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS941123
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha-2-macroglobulin-like 1 (A2ML1), ELISA Kit

 Also Known As   

Human Alpha-2-macroglobulin-like protein 1, A2ML1 ELISA Kit

 Product Synonym Names    Human Alpha-2-macroglobulin-like protein 1 (A2ML1) ELISA kit; CPAMD9; DKFZp686C1729; DKFZp686D2011; DKFZp686G1812; DKFZp686L1821; DKFZp686O1010; FLJ16045; FLJ25179; FLJ39129; FLJ41597; FLJ41598; FLJ41607; C3 and PZP-like; alpha-2-macroglobulin domain containin; alpha-2-macroglobulin-like 1
 Product Gene Name   

A2ML1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS941123 Testing Data    Testing Data PDF
 OMIM    610627
 3D Structure    ModBase 3D Structure for A8K2U0
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human A2ML1. No significant cross-reactivity or interference between Human A2ML1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    9.38 ng/ml-600 ng/ml
 Sensitivity    2.34 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of A2ML1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for A2ML1 purchase    MBS941123 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-2-macroglobulin-like 1 (A2ML1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 9.38 ng/ml-600 ng/ml in biological research samples containing A2ML1, with an estimated sensitivity of 2.34 ng/ml. The ELISA analytical biochemical technique of the MBS941123 kit is based on A2ML1 antibody-A2ML1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect A2ML1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, A2ML1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for A2ML1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for A2ML1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any A2ML1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A2ML1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A2ML1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of A2ML1 elisa kit    A2ML1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS941123. Click to request current manual
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NCBI/Uniprot data below describe general gene information for A2ML1. It may not necessarily be applicable to this product.
 NCBI GI #    308153641
 NCBI Accession #    A8K2U0.3 [Other Products]
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 UniProt Primary Accession #    A8K2U0 [Other Products]
 UniProt Secondary Accession #    Q2M224; Q6ZW52; Q6ZW53; Q8N1M4; Q96LQ8; B5MDD1 [Other Products]
 Molecular Weight    161,107 Da
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 NCBI Official Full Name    Alpha-2-macroglobulin-like protein 1
 UniProt Protein Name    Alpha-2-macroglobulin-like protein 1
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9
 Protein Family    Alpha-2-macroglobulin-like protein
 UniProt Gene Name    A2ML1 [Similar Products]
 UniProt Synonym Gene Names    CPAMD9 [Similar Products]
 UniProt Entry Name    A2ML1_HUMAN
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 NCBI Summary for A2ML1    This gene encodes a member of the alpha-macroglobulin superfamily. The encoded protein is thought to be an N-glycosylated monomeric protein that acts as an inhibitor of several proteases. It has been shown to form covalent interactions with proteases, and has been reported as the p170 antigen recognized by autoantibodies in the autoimmune disease paraneoplastic pemphigus (PNP; PMID:20805888). Mutations in these gene have also been associated with some cases of Noonan syndrome (NS; PMID:24939586) as well as some cases of otitis media (PMID:26121085). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
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 UniProt Comments for A2ML1    A2ML1: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity

Biological Process: regulation of endopeptidase activity
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with A2ML1 elisa kitPathways associated with A2ML1 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with A2ML1 and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with A2ML1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >3 publications with A2ML1 and Skin
 Testis Antibodies  >1 publications with A2ML1 and Testis
 Placenta Antibodies  >1 publications with A2ML1 and Placenta
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