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IDUA elisa kit :: Human alpha-L-iduronidase (IDUA) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS266949
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha-L-iduronidase (IDUA), ELISA Kit

★Popular Item★
 Also Known As   

Human alpha-L-iduronidase (IDUA) ELISA Kit

 Product Synonym Names    Human a-L-iduronidase (IDUA)  ELISA Kit
 Product Gene Name   

IDUA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS266949 COA    COA PDF
 OMIM    607016
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IDUA concentration
 Assay Type    Sandwich
 Detection Range    10 ng/ml-0.156 ng/ml
 Sensitivity    0.05 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IDUA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IDUA purchase    MBS266949 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-L-iduronidase (IDUA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156 ng/ml in biological research samples containing IDUA, with an estimated sensitivity of 0.05 ng/ml. The ELISA analytical biochemical technique of the MBS266949 kit is based on IDUA antibody-IDUA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IDUA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IDUA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IDUA concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IDUA elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human IDUA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of IDUA elisa kit    IDUA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS266949. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IDUA. It may not necessarily be applicable to this product.
 NCBI GI #    110611239
 NCBI GeneID    3425
 NCBI Accession #    NP_000194.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000203.4 [Other Products]
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 UniProt Related Accession #    P35475 [Other Products]
 Molecular Weight    72,670 Da
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 NCBI Official Full Name    alpha-L-iduronidase
 NCBI Official Synonym Full Names    iduronidase, alpha-L-
 NCBI Official Symbol    IDUA [Similar Products]
 NCBI Official Synonym Symbols   
IDA; MPS1
[Similar Products]
 NCBI Protein Information    alpha-L-iduronidase
 UniProt Protein Name    Alpha-L-iduronidase
 Protein Family    Alpha-L-iduronidase
 UniProt Gene Name    IDUA [Similar Products]
 UniProt Entry Name    IDUA_HUMAN
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 NCBI Summary for IDUA    This gene encodes an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans. Mutations in this gene that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I). [provided by RefSeq, Jul 2008]
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 UniProt Comments for IDUA    IDUA: Defects in IDUA are the cause of mucopolysaccharidosis type 1H (MPS1H); also known as Hurler syndrome. MPS1H is a severe form of mucopolysaccharidosis type 1, a rare lysosomal storage disease characterized by progressive physical deterioration with urinary excretion of dermatan sulfate and heparan sulfate. Patients with MPS1H usually present, within the first year of life, a combination of hepatosplenomegaly, skeletal deformities, corneal clouding and severe mental retardation. Obstructive airways disease, respiratory infection and cardiac complications usually result in death before 10 years of age. Defects in IDUA are the cause of mucopolysaccharidosis type 1H/S (MPS1H/S); also known as Hurler-Scheie syndrome. MPS1H/S is a form of mucopolysaccharidosis type 1, a rare lysosomal storage disease characterized by progressive physical deterioration with urinary excretion of dermatan sulfate and heparan sulfate. MPS1H/S represents an intermediate phenotype of the MPS1 clinical spectrum. It is characterized by relatively little neurological involvement, but most of the somatic symptoms described for severe MPS1 develop in the early to mid-teens, causing considerable loss of mobility. Defects in IDUA are the cause of mucopolysaccharidosis type 1S (MPS1S); also known as Scheie syndrome. MPS1S is a mild form of mucopolysaccharidosis type 1, a rare lysosomal storage disease characterized by progressive physical deterioration with urinary excretion of dermatan sulfate and heparan sulfate. Patients with MPS1S may have little or no neurological involvement, normal stature and life span, but present development of joints stiffness, mild hepatosplenomegaly, aortic valve disease and corneal clouding. Belongs to the glycosyl hydrolase 39 family.

Protein type: EC 3.2.1.76; Hydrolase; Glycan Metabolism - glycosaminoglycan degradation

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: lysosomal lumen

Molecular Function: L-iduronidase activity; receptor binding

Biological Process: glycosaminoglycan metabolic process; disaccharide metabolic process; dermatan sulfate catabolic process; cell morphogenesis; pathogenesis; limb morphogenesis; chemical homeostasis; chondroitin sulfate metabolic process; glycosaminoglycan catabolic process; lysosome organization and biogenesis; skeletal morphogenesis; chondroitin sulfate catabolic process; carbohydrate metabolic process

Disease: Hurler-scheie Syndrome; Scheie Syndrome; Hurler Syndrome
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 Research Articles on IDUA    1. Amino acid substitutions in alpha-L-iduronidase determine the severity of mucopolysaccharidosis type I.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IDUA elisa kitPathways associated with IDUA elisa kit
 Reference Product  PubMed Publications
 IDS elisa kit  >4 publications with IDUA and IDS
 SGSH elisa kit  >2 publications with IDUA and SGSH
 ARSB elisa kit  >1 publications with IDUA and ARSB
 Products by Pathway  Pathway Diagram
 CS/DS Degradation Pathway antibodies  CS/DS Degradation Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Dermatan Sulfate Degradation Pathway antibodies  Dermatan Sulfate Degradation Pathway Diagram
 Dermatan Sulfate Degradation Pathway antibodies  Dermatan Sulfate Degradation Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 HS-GAG Degradation Pathway antibodies  HS-GAG Degradation Pathway Diagram
 Heparan Sulfate Degradation Pathway antibodies  Heparan Sulfate Degradation Pathway Diagram
Diseases associated with IDUA elisa kitOrgans/Tissues associated with IDUA elisa kit
 Disease Name  Pubmed Publications
 Mucopolysaccharidosis I Antibodies  >120 publications with IDUA and Mucopolysaccharidosis I
 Nervous System Diseases Antibodies  >16 publications with IDUA and Nervous System Diseases
 Brain Diseases Antibodies  >15 publications with IDUA and Brain Diseases
 Heart Diseases Antibodies  >8 publications with IDUA and Heart Diseases
 Aortic Diseases Antibodies  >5 publications with IDUA and Aortic Diseases
 Movement Disorders Antibodies  >5 publications with IDUA and Movement Disorders
 Musculoskeletal Diseases Antibodies  >4 publications with IDUA and Musculoskeletal Diseases
 Kidney Diseases Antibodies  >3 publications with IDUA and Kidney Diseases
 Inflammation Antibodies  >3 publications with IDUA and Inflammation
 Drug Toxicity Antibodies  >3 publications with IDUA and Drug Toxicity
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >45 publications with IDUA and Blood
 Brain Antibodies  >41 publications with IDUA and Brain
 Bone Antibodies  >25 publications with IDUA and Bone
 Heart Antibodies  >18 publications with IDUA and Heart
 Kidney Antibodies  >11 publications with IDUA and Kidney
 Skin Antibodies  >9 publications with IDUA and Skin
 Lung Antibodies  >9 publications with IDUA and Lung
 Ovary Antibodies  >7 publications with IDUA and Ovary
 Eye Antibodies  >5 publications with IDUA and Eye
 Uterus Antibodies  >3 publications with IDUA and Uterus
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