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AMACR elisa kit :: Human alpha-methylacyl-CoA racemase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS901785
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha-methylacyl-CoA racemase (AMACR), ELISA Kit

 Also Known As   

Human Alpha-methylacyl-CoA racemase, AMACR ELISA Kit

 Product Synonym Names    Human Alpha-methylacyl-CoA racemase (AMACR) ELISA kit; CBAS4; RACE; RM; 2-methylacyl-CoA racemase; alpha-methylacyl-CoA racemase
 Product Gene Name   

AMACR elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS901785 Testing Data    Testing Data PDF
 OMIM    gene 614307
 3D Structure    ModBase 3D Structure for Q9UHK6
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human AMACR. No significant cross-reactivity or interference between Human AMACR and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml-2000 pg/ml
 Sensitivity    7.81 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AMACR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AMACR purchase    MBS901785 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-methylacyl-CoA racemase (AMACR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing AMACR, with an estimated sensitivity of 7.81 pg/ml. The ELISA analytical biochemical technique of the MBS901785 kit is based on AMACR antibody-AMACR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMACR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMACR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AMACR elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMACR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMACR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMACR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMACR bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of AMACR elisa kit    AMACR elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS901785. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AMACR. It may not necessarily be applicable to this product.
 NCBI GI #    266456254
 NCBI GeneID    23600
 NCBI Accession #    NP_001161067.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001167595.1 [Other Products]
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 UniProt Primary Accession #    Q9UHK6 [Other Products]
 UniProt Secondary Accession #    O43673; Q3KT79; Q96GH1; Q9Y3Q1; A5YM47; B8Y916; B8Y918; F8W9N1 [Other Products]
 UniProt Related Accession #    Q9UHK6 [Other Products]
 Molecular Weight    42,387 Da
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 NCBI Official Full Name    alpha-methylacyl-CoA racemase isoform 3
 NCBI Official Synonym Full Names    alpha-methylacyl-CoA racemase
 NCBI Official Symbol    AMACR [Similar Products]
 NCBI Official Synonym Symbols   
RM; RACE; CBAS4; AMACRD
[Similar Products]
 NCBI Protein Information    alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase
 UniProt Protein Name    Alpha-methylacyl-CoA racemase
 UniProt Synonym Protein Names   
2-methylacyl-CoA racemase
 Protein Family    Alpha-methylacyl-CoA racemase
 UniProt Gene Name    AMACR [Similar Products]
 UniProt Entry Name    AMACR_HUMAN
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 NCBI Summary for AMACR    This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
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 UniProt Comments for AMACR    AMACR: Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. Belongs to the CaiB/BaiF CoA-transferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.1.99.4; Isomerase; Mitochondrial; Lipid Metabolism - primary bile acid biosynthesis

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: peroxisomal matrix; mitochondrion; cytoplasm; peroxisome

Molecular Function: receptor binding; alpha-methylacyl-CoA racemase activity

Biological Process: bile acid biosynthetic process; fatty acid beta-oxidation using acyl-CoA oxidase; bile acid metabolic process; cellular lipid metabolic process

Disease: Bile Acid Synthesis Defect, Congenital, 4; Alpha-methylacyl-coa Racemase Deficiency
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 Research Articles on AMACR    1. AMACR should be the first-line positive marker for confirmation of a diagnosis of minimal adenocarcinoma of the prostate.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with AMACR elisa kitDiseases associated with AMACR elisa kit
 Products by Pathway  Pathway Diagram
 Beta-oxidation Of Pristanoyl-CoA Pathway antibodies  Beta-oxidation Of Pristanoyl-CoA Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Peroxisomal Lipid Metabolism Pathway antibodies  Peroxisomal Lipid Metabolism Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Primary Bile Acid Biosynthesis Pathway antibodies  Primary Bile Acid Biosynthesis Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >246 publications with AMACR and Neoplasms
 Prostatic Neoplasms Antibodies  >178 publications with AMACR and Prostatic Neoplasms
 Carcinoma Antibodies  >161 publications with AMACR and Carcinoma
 Adenocarcinoma Antibodies  >135 publications with AMACR and Adenocarcinoma
 Kidney Neoplasms Antibodies  >36 publications with AMACR and Kidney Neoplasms
 Kidney Diseases Antibodies  >33 publications with AMACR and Kidney Diseases
 Hyperplasia Antibodies  >30 publications with AMACR and Hyperplasia
 Colorectal Neoplasms Antibodies  >27 publications with AMACR and Colorectal Neoplasms
 Adenoma Antibodies  >26 publications with AMACR and Adenoma
 Recurrence Antibodies  >11 publications with AMACR and Recurrence
Organs/Tissues associated with AMACR elisa kit
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >194 publications with AMACR and Prostate
 Kidney Antibodies  >38 publications with AMACR and Kidney
 Blood Antibodies  >29 publications with AMACR and Blood
 Lung Antibodies  >11 publications with AMACR and Lung
 Liver Antibodies  >10 publications with AMACR and Liver
 Bladder Antibodies  >9 publications with AMACR and Bladder
 Intestine Antibodies  >8 publications with AMACR and Intestine
 Lymph Antibodies  >6 publications with AMACR and Lymph
 Lymph Node Antibodies  >5 publications with AMACR and Lymph Node
 Skin Antibodies  >5 publications with AMACR and Skin
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