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Abeta1-42 elisa kit :: Human amyloid beta peptide 1-42 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS729541
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

amyloid beta peptide 1-42 (Abeta1-42), ELISA Kit

★Popular Item★
 Also Known As   

Human amyloid beta peptide 1-42 ELISA Kit

 Product Synonym Names    Human amyloid beta peptide 1-42 ELISA Kit; amyloid beta peptide 1-42; amyloid beta peptide 1-42 (Human)
 Product Gene Name   

Abeta1-42 elisa kit

[Similar Products]
 Product Synonym Gene Name    Ab1-42; Aβ1-42 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 16; NC_000082.6 (84954436..85173707, complement). Location: 16 C3-qter; 16 46.92 cM
 3D Structure    ModBase 3D Structure for P12023
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ?AP1~42. No significant cross-reactivity or interference between ?AP1~42 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ?AP1~42 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This ?AP1~42 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ?AP1~42. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Abeta1-42 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Abeta1-42 purchase    MBS729541 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the amyloid beta peptide 1-42 (Abeta1-42) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Abeta1-42, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS729541 kit is based on Abeta1-42 antibody-Abeta1-42 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Abeta1-42 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Abeta1-42. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Abeta1-42 elisa kit

   Principle of the assay: ?AP1~42 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-?AP1~42 antibody and an ?AP1~42-HRP conjugate. The assay sample and buffer are incubated together with ?AP1~42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ?AP1~42 concentration since ?AP1~42 from samples and ?AP1~42-HRP conjugate compete for the anti-?AP1~42 antibody binding site. Since the number of sites is limited, as more sites are occupied by ?AP1~42 from the sample, fewer sites are left to bind ?AP1~42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ?AP1~42 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Abeta1-42 elisa kit    Human ELISA Kit
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 Typical Testing Data/Standard Curve (for reference only) of Abeta1-42 elisa kit    Abeta1-42 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS729541. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Abeta1-42. It may not necessarily be applicable to this product.
 NCBI GI #    311893408
 NCBI GeneID    11820
 NCBI Accession #    NP_001185755.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001198826.1 [Other Products]
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 UniProt Primary Accession #    P12023 [Other Products]
 UniProt Secondary Accession #    P97487; P97942; Q99K32 [Other Products]
 UniProt Related Accession #    P12023 [Other Products]
 Molecular Weight    86,722 Da
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 NCBI Official Full Name    amyloid beta A4 protein isoform 6
 NCBI Official Synonym Full Names    amyloid beta (A4) precursor protein
 NCBI Official Symbol    App [Similar Products]
 NCBI Official Synonym Symbols   
Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik
[Similar Products]
 NCBI Protein Information    amyloid beta A4 protein; appican; protease nexin II; amyloidogenic glycoprotein; alzheimer disease amyloid A4 protein homolog
 UniProt Protein Name    Amyloid beta A4 protein
 UniProt Synonym Protein Names   
ABPP; APP; Alzheimer disease amyloid A4 protein homolog; Amyloidogenic glycoprotein; AGCleaved into the following 14 chains:N-APPSoluble APP-alpha; S-APP-alphaSoluble APP-beta; S-APP-betaC99; Alternative name(s):; APP-C99Beta-amyloid protein 42; Alternative name(s):; Beta-APP42Beta-amyloid protein 40; Alternative name(s):; Beta-APP40C83P3(42)P3(40)C80Gamma-secretase C-terminal fragment 59; Alternative name(s):; APP-C59; Amyloid intracellular domain 59; AID(59); Gamma-CTF(59)Gamma-secretase C-terminal fragment 57; Alternative name(s):; APP-C57; Amyloid intracellular domain 57; AID(57); Gamma-CTF(57)Gamma-secretase C-terminal fragment 50; Alternative name(s):; Amyloid intracellular domain 50; AID(50); Gamma-CTF(50)
 UniProt Gene Name    App [Similar Products]
 UniProt Synonym Gene Names    APP; AG; S-APP-alpha; S-APP-beta; AID(59); AID(57); AID(50) [Similar Products]
 UniProt Entry Name    A4_MOUSE
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 UniProt Comments for Abeta1-42    APP: a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation. The Abeta peptide is released from the cell, its extracellular deposition and accumulation form the main components of amyloid plaques in Alzheimer's disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis. Can promote transcription activation through binding to Fe65-Tip60 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(O) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. Induces a RAGE-dependent pathway that activates p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, JIP1, SHC1 and, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Amyloid beta-42 binds nAChRA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. 10 isoforms of the human protein are produced by alternative splicing. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Belongs to the APP family.

Protein type: Apoptosis; Transcription factor; Receptor, misc.; Membrane protein, integral; Cell surface

Cellular Component: Golgi apparatus; extracellular space; cell surface; smooth endoplasmic reticulum; integral to membrane; terminal button; coated pit; intercellular junction; ER to Golgi transport vesicle; lipid raft; ciliary rootlet; membrane; nuclear envelope lumen; perinuclear region of cytoplasm; cytoplasm; intracellular; cytoplasmic vesicle; neuromuscular junction; receptor complex; endosome; neuron projection; intracellular membrane-bound organelle; cell; axon; apical part of cell; plasma membrane; spindle midzone

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; identical protein binding; protein binding; enzyme binding; protease activator activity; DNA binding; transition metal ion binding; protease inhibitor activity; metal ion binding; PTB domain binding; acetylcholine receptor binding; receptor binding

Biological Process: extracellular matrix organization and biogenesis; apoptosis; adult locomotory behavior; negative regulation of peptidase activity; locomotory behavior; mRNA polyadenylation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of translation; synaptic growth at neuromuscular junction; forebrain development; dendrite development; collateral sprouting in the absence of injury; visual learning; cell adhesion; neuromuscular process controlling balance; neurite development; protein homooligomerization; nervous system development; cholesterol metabolic process; Notch signaling pathway; cellular copper ion homeostasis; regulation of epidermal growth factor receptor activity; mating behavior; axon cargo transport; regulation of multicellular organism growth; endocytosis; axon midline choice point recognition; smooth endoplasmic reticulum calcium ion homeostasis; negative regulation of neuron differentiation; neuron apoptosis; axonogenesis; suckling behavior; ionotropic glutamate receptor signaling pathway; regulation of gene expression; regulation of protein binding; regulation of synapse structure and activity; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; neuron remodeling
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 Research Articles on Abeta1-42    1. apoE influences sAbeta metabolism not through direct binding to sAbeta in solution but through its actions with other interacting receptors/transporters and cell surfaces
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Abeta1-42 elisa kitDiseases associated with Abeta1-42 elisa kit
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4683 publications with Abeta1-42 and Nervous System Diseases
 Dementia Antibodies  >4530 publications with Abeta1-42 and Dementia
 Brain Diseases Antibodies  >4470 publications with Abeta1-42 and Brain Diseases
 Alzheimer Disease Antibodies  >4338 publications with Abeta1-42 and Alzheimer Disease
 Neurodegenerative Diseases Antibodies  >4102 publications with Abeta1-42 and Neurodegenerative Diseases
 Disease Models, Animal Antibodies  >1388 publications with Abeta1-42 and Disease Models, Animal
 Plaque, Amyloid Antibodies  >1012 publications with Abeta1-42 and Plaque, Amyloid
 Neoplasms Antibodies  >762 publications with Abeta1-42 and Neoplasms
 Inflammation Antibodies  >512 publications with Abeta1-42 and Inflammation
 Drug Toxicity Antibodies  >366 publications with Abeta1-42 and Drug Toxicity
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