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APOA2 elisa kit :: Human apolipoprotein A-II ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704270
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

apolipoprotein A-II (APOA2), ELISA Kit

 Also Known As   

Human Apolipoprotein A2, apo-A2 ELISA Kit

 Product Synonym Names    Human Apolipoprotein A2; apo-A2 ELISA Kit; apolipoprotein A-II
 Product Gene Name   

APOA2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704270 Testing Data    Testing Data PDF
 OMIM    107670
 3D Structure    ModBase 3D Structure for P02652
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human APOA2. No significant cross-reactivity or interference between Human APOA2 and analogues was observed.
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 Detection Range    6.2 ug/ml-400 ug/ml?
 Sensitivity    1.6 ug/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Immunity
 Protein Biological Process 3    Host-virus interaction
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of APOA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for APOA2 purchase    MBS704270 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein A-II (APOA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.2 ug/ml-400 ug/ml? in biological research samples containing APOA2, with an estimated sensitivity of 1.6 ug/ml. The ELISA analytical biochemical technique of the MBS704270 kit is based on APOA2 antibody-APOA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for APOA2 elisa kit

   Introduction: Apolipoprotein A2 is a 17.5 kDa protein with a pI of 4.9. Lipids, as such areinsoluble in plasma. They combine with apolipoproteins to form lipoproteinsand this process solubilises lipids in plasma, providing a transport systemfor lipids. Apolipoproteins also play an important role in lipoprotein receptorrecognition and regulation of certain enzymes in lipoprotein metabolism.Two main forms of high density lipoprotein exist; both contain APOA1, butone type only contains APO A2.For more information on APO A2 pleasedownload the PDF catalogue below.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to apo-A2. Standards or samples are then added to theappropriate microtiter plate wells with a biotin-conjugated polyclonalantibody preparation specific for apo-A2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well andincubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solutionis added to each well. Only those wells that contain apo-A2,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit achange in color. The enzyme-substrate reaction is terminated by theaddition of a sulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of apo-A2 in the samples is then determined by comparingthe O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of APOA2 elisa kit    APOA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704270. Click to request current manual
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NCBI/Uniprot data below describe general gene information for APOA2. It may not necessarily be applicable to this product.
 NCBI GI #    4502149
 NCBI GeneID    336
 NCBI Accession #    NP_001634.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001643.1 [Other Products]
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 UniProt Primary Accession #    P02652 [Other Products]
 UniProt Secondary Accession #    B2R524 [Other Products]
 UniProt Related Accession #    P02652 [Other Products]
 Molecular Weight    11,175 Da
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 NCBI Official Full Name    apolipoprotein A-II preproprotein
 NCBI Official Synonym Full Names    apolipoprotein A-II
 NCBI Official Symbol    APOA2 [Similar Products]
 NCBI Official Synonym Symbols   
apoAII; Apo-AII; ApoA-II
[Similar Products]
 NCBI Protein Information    apolipoprotein A-II; apolipoprotein A2
 UniProt Protein Name    Apolipoprotein A-II
 UniProt Synonym Protein Names   
Apolipoprotein A2Cleaved into the following 2 chains:Proapolipoprotein A-II; ProapoA-II; Truncated apolipoprotein A-IIAlternative name(s):Apolipoprotein A-II(1-76)
 Protein Family    Apolipoprotein
 UniProt Gene Name    APOA2 [Similar Products]
 UniProt Synonym Gene Names    Apo-AII; ApoA-II; ProapoA-II [Similar Products]
 UniProt Entry Name    APOA2_HUMAN
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 NCBI Summary for APOA2    This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for APOA2    Function: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
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 Research Articles on APOA2    1. Apolipoprotein A-II and the regulation of high density lipoproteins in cardiovascular disease. [Review]
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with APOA2 elisa kitPathways associated with APOA2 elisa kit
 Reference Product  PubMed Publications
 APOA1 elisa kit  >38 publications with APOA2 and APOA1
 APOE elisa kit  >15 publications with APOA2 and APOE
 APOB elisa kit  >15 publications with APOA2 and APOB
 APOC2 elisa kit  >11 publications with APOA2 and APOC2
 APOC3 elisa kit  >9 publications with APOA2 and APOC3
 APOA4 elisa kit  >4 publications with APOA2 and APOA4
 LPL elisa kit  >4 publications with APOA2 and LPL
 APOA5 elisa kit  >4 publications with APOA2 and APOA5
 APOH elisa kit  >2 publications with APOA2 and APOH
 Products by Pathway  Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
Diseases associated with APOA2 elisa kitOrgans/Tissues associated with APOA2 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >20 publications with APOA2 and Cardiovascular Diseases
 Dyslipidemias Antibodies  >16 publications with APOA2 and Dyslipidemias
 Liver Diseases Antibodies  >12 publications with APOA2 and Liver Diseases
 Hyperlipidemias Antibodies  >12 publications with APOA2 and Hyperlipidemias
 Heart Diseases Antibodies  >12 publications with APOA2 and Heart Diseases
 Inflammation Antibodies  >8 publications with APOA2 and Inflammation
 Kidney Diseases Antibodies  >6 publications with APOA2 and Kidney Diseases
 Carcinoma, Hepatocellular Antibodies  >5 publications with APOA2 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >5 publications with APOA2 and Liver Neoplasms
 Hypertriglyceridemia Antibodies  >5 publications with APOA2 and Hypertriglyceridemia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >52 publications with APOA2 and Blood
 Liver Antibodies  >19 publications with APOA2 and Liver
 Heart Antibodies  >13 publications with APOA2 and Heart
 Kidney Antibodies  >5 publications with APOA2 and Kidney
 Intestine Antibodies  >4 publications with APOA2 and Intestine
 Muscle Antibodies  >3 publications with APOA2 and Muscle
 Brain Antibodies  >3 publications with APOA2 and Brain
 Placenta Antibodies  >3 publications with APOA2 and Placenta
 Lung Antibodies  >2 publications with APOA2 and Lung
 Spleen Antibodies  >2 publications with APOA2 and Spleen
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