Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow APOB rightarrow Human APOB  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • CXCR7 Blocking Peptide
 • Aflatoxin M1, B1 Antibody
 • ANGPTL5 Antibody
 • C-type lectin domain family 4 member E (Clec4e) Recombinant Protein
 • G-CSF (CSF3) Antibody
 • Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) Antibody
 • reproductive and respiratory syndrome virus (PRRSV) antibody... ELISA Kit
 • ZNF136 Antibody
 • Bile acid receptor (NR1H4) ELISA Kit
 • Matrix Gla Protein (MGP) ELISA Kit
 • KlenThermase DNA Polymerase Taq DNA Polymerase
 • microtubule-associated protein 1 light chain 3 beta (MAP1LC3... ELISA Kit
 • Actinin, alpha 1 (ACTN1) Antibody
 • RET Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

APOB elisa kit :: Human apolipoprotein B (including Ag (x) antigen) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701268 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $375 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,295 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,330 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

apolipoprotein B (including Ag (x) antigen) (APOB), ELISA Kit

★Popular Item★
 Also Known As   

Human apolipoprotein B100, Apo-B100 ELISA Kit

 Product Synonym Names    Human apolipoprotein B100 (Apo-B100) ELISA Kit; FLDB; LDLCQ4; apoB-100; apoB-48; apolipoprotein B; apolipoprotein B48; Human apolipoprotein B100 (Apo-B100)
 Product Gene Name   

APOB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701268 Testing Data    Testing Data PDF
 OMIM    615558
 3D Structure    ModBase 3D Structure for P04114
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Human APOB. No significant cross-reactivity or interference between Human APOB and analogues was observed.
Section Bar
 Samples    Serum, plasma, cell culture supernates, saliva, urine
 Detection Range    78 ng/ml-5000 ng/ml
 Sensitivity    19.5 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Apolipoprotein
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of APOB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for APOB purchase    MBS701268 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein B (including Ag (x) antigen) (APOB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78 ng/ml-5000 ng/ml in biological research samples containing APOB, with an estimated sensitivity of 19.5 ng/ml. The ELISA analytical biochemical technique of the MBS701268 kit is based on APOB antibody-APOB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, saliva, urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for APOB elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APO-B100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated polyclonal antibody preparation specific for APO-B100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain APO-B100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of APO-B100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of APOB elisa kit    APOB elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS701268. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for APOB. It may not necessarily be applicable to this product.
 NCBI GI #    105990532
 NCBI GeneID    338
 NCBI Accession #    NP_000375.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000384.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P04114 [Other Products]
 UniProt Secondary Accession #    O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8 [Other Products]
 UniProt Related Accession #    P04114 [Other Products]
 Molecular Weight    515,605 Da
Table BarTOPTable Bar
 NCBI Official Full Name    apolipoprotein B-100
 NCBI Official Synonym Full Names    apolipoprotein B
 NCBI Official Symbol    APOB [Similar Products]
 NCBI Official Synonym Symbols   
FLDB; LDLCQ4
[Similar Products]
 NCBI Protein Information    apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
 UniProt Protein Name    Apolipoprotein B-100
 Protein Family    Apolipoprotein
 UniProt Gene Name    APOB [Similar Products]
 UniProt Synonym Gene Names    Apo B-100; Apo B-48 [Similar Products]
 UniProt Entry Name    APOB_HUMAN
Section Bar
 NCBI Summary for APOB    This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for APOB    Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Table BarTOPTable Bar
 Research Articles on APOB    1. XbaI and EcoRI polymorphisms were associated with presence of plaques on carotid arteries. Plasma ApoB level was not independently associated with ultrasonographic phenotypes of carotid atherosclerosis in patients with diabetes mellitus.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with APOB elisa kitPathways associated with APOB elisa kit
 Reference Product  PubMed Publications
 APOA1 elisa kit  >369 publications with APOB and APOA1
 LDLR elisa kit  >322 publications with APOB and LDLR
 LIPC elisa kit  >26 publications with APOB and LIPC
 MTTP elisa kit  >23 publications with APOB and MTTP
 BGN elisa kit  >8 publications with APOB and BGN
 HSPA8 elisa kit  >1 publications with APOB and HSPA8
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 LDL-mediated Lipid Transport Pathway antibodies  LDL-mediated Lipid Transport Pathway Diagram
Diseases associated with APOB elisa kitOrgans/Tissues associated with APOB elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3405 publications with APOB and Cardiovascular Diseases
 Dyslipidemias Antibodies  >2969 publications with APOB and Dyslipidemias
 Atherosclerosis Antibodies  >2534 publications with APOB and Atherosclerosis
 Hyperlipidemias Antibodies  >2452 publications with APOB and Hyperlipidemias
 Coronary Disease Antibodies  >1853 publications with APOB and Coronary Disease
 Heart Diseases Antibodies  >1598 publications with APOB and Heart Diseases
 Hypercholesterolemia Antibodies  >1473 publications with APOB and Hypercholesterolemia
 Hyperlipoproteinemia Type II Antibodies  >718 publications with APOB and Hyperlipoproteinemia Type II
 Hypertriglyceridemia Antibodies  >623 publications with APOB and Hypertriglyceridemia
 Liver Diseases Antibodies  >575 publications with APOB and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7360 publications with APOB and Blood
 Liver Antibodies  >2021 publications with APOB and Liver
 Heart Antibodies  >1258 publications with APOB and Heart
 Intestine Antibodies  >544 publications with APOB and Intestine
 Kidney Antibodies  >374 publications with APOB and Kidney
 Muscle Antibodies  >285 publications with APOB and Muscle
 Brain Antibodies  >122 publications with APOB and Brain
 Lung Antibodies  >105 publications with APOB and Lung
 Embryonic Tissue Antibodies  >52 publications with APOB and Embryonic Tissue
 Spleen Antibodies  >50 publications with APOB and Spleen
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions