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beta-APP elisa kit :: Human beta-Amyloid precursor protein (beta-APP) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705628
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

beta-Amyloid precursor protein (beta-APP), ELISA Kit

★Popular Item★
 Also Known As   

Human beta-Amyloid precursor protein, beta-APP ELISA Kit

 Product Synonym Names    Human beta-Amyloid precursor protein (beta-APP) ELISA kit; beta-Amyloid precursor protein (beta-APP)
 Product Gene Name   

beta-APP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705628 Testing Data    Testing Data PDF
 OMIM    gene 605714
 3D Structure    ModBase 3D Structure for P05067
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human beta-APP. No significant cross-reactivity or interference between human beta-APP and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    47 ng/ml-3000 ng/ml.
 Sensitivity    The minimum detectable dose of human beta-APP is typically less than 11.75 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of beta-APP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for beta-APP purchase    MBS705628 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the beta-Amyloid precursor protein (beta-APP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 47 ng/ml-3000 ng/ml in biological research samples containing beta-APP, with an estimated sensitivity of The minimum detectable dose of human beta-APP is typically less than 11.75 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS705628 kit is based on beta-APP antibody-beta-APP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect beta-APP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, beta-APP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for beta-APP elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-APP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-APP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-APP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-APP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of beta-APP elisa kit    beta-APP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705628. Click to request current manual
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NCBI/Uniprot data below describe general gene information for beta-APP. It may not necessarily be applicable to this product.
 NCBI GI #    8176534
 NCBI GeneID    351
 NCBI Accession #    AAB26265.2 [Other Products]
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 UniProt Primary Accession #    P05067 [Other Products]
 UniProt Secondary Accession #    P09000; P78438; Q13764; Q13778; Q13793; Q16011; B2R5V1; B4DII8; D3DSD1; D3DSD2; D3DSD3 [Other Products]
 UniProt Related Accession #    P05067 [Other Products]
 Molecular Weight    84,521 Da
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 NCBI Official Full Name    beta-amyloid peptide, partial
 NCBI Official Synonym Full Names    amyloid beta (A4) precursor protein
 NCBI Official Symbol    APP [Similar Products]
 NCBI Official Synonym Symbols   
AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
[Similar Products]
 NCBI Protein Information    amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); alzheimer disease amyloid protein; cerebral vascular amyloid peptide
 UniProt Protein Name    Amyloid beta A4 protein
 UniProt Synonym Protein Names   
ABPP; APPI; APP; Alzheimer disease amyloid protein; Cerebral vascular amyloid peptide; CVAP; PreA4; Protease nexin-II; PN-II
 UniProt Gene Name    APP [Similar Products]
 UniProt Synonym Gene Names    A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50) [Similar Products]
 UniProt Entry Name    A4_HUMAN
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 NCBI Summary for beta-APP    This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]
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 UniProt Comments for beta-APP    Function: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu2+ ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.5 PublicationsManual assertion based on experiment in:Ref.37
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 Research Articles on beta-APP    1. Study described hyperlocomotion, reduced anxiety, and an impairment in spatial memory in the cheeseboard task in female APPxPS1 mice
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with beta-APP elisa kitPathways associated with beta-APP elisa kit
 Reference Product  PubMed Publications
 BACE1 elisa kit  >911 publications with beta-APP and BACE1
 PSEN1 elisa kit  >512 publications with beta-APP and PSEN1
 APOE elisa kit  >405 publications with beta-APP and APOE
 PSEN2 elisa kit  >226 publications with beta-APP and PSEN2
 ACHE elisa kit  >122 publications with beta-APP and ACHE
 CASP3 elisa kit  >119 publications with beta-APP and CASP3
 APBB1 elisa kit  >97 publications with beta-APP and APBB1
 BACE2 elisa kit  >84 publications with beta-APP and BACE2
 IDE elisa kit  >33 publications with beta-APP and IDE
 APBA1 elisa kit  >33 publications with beta-APP and APBA1
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
Diseases associated with beta-APP elisa kitOrgans/Tissues associated with beta-APP elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >5167 publications with beta-APP and Nervous System Diseases
 Brain Diseases Antibodies  >4927 publications with beta-APP and Brain Diseases
 Alzheimer Disease Antibodies  >4877 publications with beta-APP and Alzheimer Disease
 Dementia Antibodies  >4553 publications with beta-APP and Dementia
 Neurodegenerative Diseases Antibodies  >4535 publications with beta-APP and Neurodegenerative Diseases
 Disease Models, Animal Antibodies  >1608 publications with beta-APP and Disease Models, Animal
 Plaque, Amyloid Antibodies  >1148 publications with beta-APP and Plaque, Amyloid
 Inflammation Antibodies  >581 publications with beta-APP and Inflammation
 Memory Disorders Antibodies  >325 publications with beta-APP and Memory Disorders
 Necrosis Antibodies  >296 publications with beta-APP and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4187 publications with beta-APP and Brain
 Blood Antibodies  >1393 publications with beta-APP and Blood
 Nerve Antibodies  >952 publications with beta-APP and Nerve
 Vascular Antibodies  >469 publications with beta-APP and Vascular
 Liver Antibodies  >440 publications with beta-APP and Liver
 Kidney Antibodies  >289 publications with beta-APP and Kidney
 Muscle Antibodies  >289 publications with beta-APP and Muscle
 Intestine Antibodies  >204 publications with beta-APP and Intestine
 Heart Antibodies  >167 publications with beta-APP and Heart
 Lung Antibodies  >140 publications with beta-APP and Lung
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