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BHMT elisa kit :: Human betaine-homocysteine methyltransferase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS904226
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

betaine-homocysteine methyltransferase (BHMT), ELISA Kit

★Popular Item★
 Also Known As   

Human Betaine--homocysteine S-methyltransferase 1, BHMT ELISA Kit

 Product Synonym Names    Human Betaine--homocysteine S-methyltransferase 1 (BHMT) ELISA kit; betaine homocysteine methyltransferase; betaine-homocysteine methyltransferase
 Product Gene Name   

BHMT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS904226 Testing Data    Testing Data PDF
 OMIM    602888
 3D Structure    ModBase 3D Structure for Q93088
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human BHMT. No significant cross-reactivity or interference between human BHMT and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of human BHMT is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BHMT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BHMT purchase    MBS904226 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the betaine-homocysteine methyltransferase (BHMT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing BHMT, with an estimated sensitivity of The minimum detectable dose of human BHMT is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS904226 kit is based on BHMT antibody-BHMT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BHMT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BHMT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BHMT elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BHMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BHMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BHMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BHMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of BHMT elisa kit    BHMT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS904226. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BHMT. It may not necessarily be applicable to this product.
 NCBI GI #    157266337
 NCBI GeneID    635
 NCBI Accession #    NP_001704.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001713.2 [Other Products]
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 UniProt Primary Accession #    Q93088 [Other Products]
 UniProt Secondary Accession #    Q9UNI9 [Other Products]
 UniProt Related Accession #    Q93088 [Other Products]
 Molecular Weight    44,998 Da
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 NCBI Official Full Name    betaine--homocysteine S-methyltransferase 1
 NCBI Official Synonym Full Names    betaine--homocysteine S-methyltransferase
 NCBI Official Symbol    BHMT [Similar Products]
 NCBI Official Synonym Symbols   
BHMT1; HEL-S-61p
[Similar Products]
 NCBI Protein Information    betaine--homocysteine S-methyltransferase 1; epididymis secretory sperm binding protein Li 61p
 UniProt Protein Name    Betaine--homocysteine S-methyltransferase 1
 Protein Family    Betaine--homocysteine S-methyltransferase
 UniProt Gene Name    BHMT [Similar Products]
 UniProt Entry Name    BHMT1_HUMAN
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 NCBI Summary for BHMT    This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for BHMT    BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; EC 2.1.1.5

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding

Biological Process: S-methylmethionine cycle; protein amino acid methylation; betaine catabolic process; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; regulation of homocysteine metabolic process; betaine metabolic process
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 Research Articles on BHMT    1. Women harboring the single nucleotide polymorphism BHMT 742G>A have a decreased risk of a Down Syndrome pregnancy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with BHMT elisa kitDiseases associated with BHMT elisa kit
 Products by Pathway  Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 One Carbon Metabolism Pathway antibodies  One Carbon Metabolism Pathway Diagram
 Sulfur Amino Acid Metabolism Pathway antibodies  Sulfur Amino Acid Metabolism Pathway Diagram
 Glycine Betaine Degradation Pathway antibodies  Glycine Betaine Degradation Pathway Diagram
 Methionine Salvage II (mammalia) Pathway antibodies  Methionine Salvage II (mammalia) Pathway Diagram
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >21 publications with BHMT and Liver Diseases
 Neoplasms Antibodies  >17 publications with BHMT and Neoplasms
 Cardiovascular Diseases Antibodies  >17 publications with BHMT and Cardiovascular Diseases
 Carcinoma Antibodies  >14 publications with BHMT and Carcinoma
 Vascular Diseases Antibodies  >12 publications with BHMT and Vascular Diseases
 Fatty Liver Antibodies  >11 publications with BHMT and Fatty Liver
 Nervous System Diseases Antibodies  >10 publications with BHMT and Nervous System Diseases
 Carcinoma, Hepatocellular Antibodies  >7 publications with BHMT and Carcinoma, Hepatocellular
 Adenocarcinoma Antibodies  >7 publications with BHMT and Adenocarcinoma
 Kidney Diseases Antibodies  >7 publications with BHMT and Kidney Diseases
Organs/Tissues associated with BHMT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >98 publications with BHMT and Liver
 Kidney Antibodies  >25 publications with BHMT and Kidney
 Brain Antibodies  >5 publications with BHMT and Brain
 Intestine Antibodies  >3 publications with BHMT and Intestine
 Eye Antibodies  >3 publications with BHMT and Eye
 Embryonic Tissue Antibodies  >1 publications with BHMT and Embryonic Tissue
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