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BTN3A1 elisa kit :: Human butyrophilin, subfamily 3, member A1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS930207
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

butyrophilin, subfamily 3, member A1 (BTN3A1), ELISA Kit

★Popular Item★
 Also Known As   

Human Butyrophilin subfamily 3 member A1, BTN3A1 ELISA Kit

 Product Synonym Names    Human Butyrophilin subfamily 3 member A1 (BTN3A1) ELISA kit; RP1-45P21.5; BT3.1; BTF5; CD277; MGC141880; dJ45P21.3 (butyrophilin; subfamily 3; member A1) ; butyrophilin; subfamily 3; member A1
 Product Gene Name   

BTN3A1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS930207 Testing Data    Testing Data PDF
 OMIM    613593
 3D Structure    ModBase 3D Structure for O00481
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human BTN3A1. No significant cross-reactivity or interference between human BTN3A1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    0.625 ng/ml -40 ng/ml
 Sensitivity    Typically less than 0.156 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BTN3A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BTN3A1 purchase    MBS930207 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the butyrophilin, subfamily 3, member A1 (BTN3A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml -40 ng/ml in biological research samples containing BTN3A1, with an estimated sensitivity of Typically less than 0.156 ng/ml. The ELISA analytical biochemical technique of the MBS930207 kit is based on BTN3A1 antibody-BTN3A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BTN3A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BTN3A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BTN3A1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BTN3A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BTN3A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BTN3A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BTN3A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of BTN3A1 elisa kit    BTN3A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS930207. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BTN3A1. It may not necessarily be applicable to this product.
 NCBI GI #    222418658
 NCBI GeneID    11119
 NCBI Accession #    NP_001138480.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145008.1 [Other Products]
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 UniProt Primary Accession #    O00481 [Other Products]
 UniProt Secondary Accession #    Q0P515; Q147X5; Q53F15; Q99420; Q9HCY1; A2A278; A8K2C8; B4DIQ1; B4DRM2; E9PGB4; E9PHG8 [Other Products]
 UniProt Related Accession #    O00481 [Other Products]
 Molecular Weight    57,677 Da
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 NCBI Official Full Name    butyrophilin subfamily 3 member A1 isoform c
 NCBI Official Synonym Full Names    butyrophilin, subfamily 3, member A1
 NCBI Official Symbol    BTN3A1 [Similar Products]
 NCBI Official Synonym Symbols   
BTF5; BT3.1; CD277; BTN3.1
[Similar Products]
 NCBI Protein Information    butyrophilin subfamily 3 member A1; dJ45P21.3 (butyrophilin, subfamily 3, member A1)
 UniProt Protein Name    Butyrophilin subfamily 3 member A1
 UniProt Synonym Protein Names   
CD_antigen: CD277
 Protein Family    Butyrophilin subfamily
 UniProt Gene Name    BTN3A1 [Similar Products]
 UniProt Synonym Gene Names    BTF5 [Similar Products]
 UniProt Entry Name    BT3A1_HUMAN
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 NCBI Summary for BTN3A1    The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010]
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 UniProt Comments for BTN3A1    BTN3A1: Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate. Belongs to the immunoglobulin superfamily. BTN/MOG family. Homodimer. 3 isoforms of the human protein are produced by alternative splicing

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding

Biological Process: activated T cell proliferation; cytokine secretion; T cell receptor signaling pathway
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 Research Articles on BTN3A1    1. These studies demonstrate that internal sensing of changes in pAg metabolite concentrations by BTN3A1 molecules is a critical step in Vgamma9Vdelta2 T cell detection of infection and tumorigenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BTN3A1 elisa kitDiseases associated with BTN3A1 elisa kit
 Reference Product  PubMed Publications
 BTN3A3 elisa kit  >5 publications with BTN3A1 and BTN3A3
 BTN3A2 elisa kit  >4 publications with BTN3A1 and BTN3A2
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1 publications with BTN3A1 and Inflammation
Organs/Tissues associated with BTN3A1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with BTN3A1 and Blood
 Mammary Gland Antibodies  >1 publications with BTN3A1 and Mammary Gland
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