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PDE5A elisa kit :: Human cGMP-specific 3',5'-cyclic phosphodiesterase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765664
Unit / Price
48-Strip-Wells  /  $295 +1 FREE 8GB USB
96-Strip-Wells  /  $390 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
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 Product Name   

cGMP-specific 3',5'-cyclic phosphodiesterase (PDE5A), ELISA Kit

★Popular Item★
 Also Known As   

Human cGMP-specific 3',5'-cyclic phosphodiesterase ELISA Kit

 Product Synonym Names    PDE5A (cGMP-specific 3',5'-cyclic phosphodiesterase/)/cGMP-binding cGMP-specific phosphodiesterase (CGB-PDE)/PDE5
 Product Gene Name   

PDE5A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS765664 COA    COA PDF
 Species Reactivity    Human
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.156-10ng/ml
 Sensitivity    0.094ng/ml
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PDE5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PDE5A purchase    MBS765664 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cGMP-specific 3',5'-cyclic phosphodiesterase (PDE5A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing PDE5A, with an estimated sensitivity of 0.094ng/ml. The ELISA analytical biochemical technique of the MBS765664 kit is based on PDE5A antibody-PDE5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDE5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDE5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PDE5A elisa kit

   Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PDE5A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PDE5A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PDE5A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PDE5A can be calculated.
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 Typical Testing Data of PDE5A elisa kit    PDE5A elisa kit Typical Testing Data image
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 Typical Standard Curve of PDE5A elisa kit    PDE5A elisa kit Typical Standard Curve image
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Sample Manual Insert of MBS765664. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PDE5A. It may not necessarily be applicable to this product.
 NCBI GI #    118404272
 NCBI GeneID    780273
 NCBI Accession #    NP_001072812.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001079344.1 [Other Products]
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 NCBI Official Full Name    cGMP-specific 3',5'-cyclic phosphodiesterase
 NCBI Official Synonym Full Names    phosphodiesterase 5A
 NCBI Official Symbol    pde5a [Similar Products]
 NCBI Official Synonym Symbols   
cn5a; pde5; pde5a1; cgb-pde
[Similar Products]
 NCBI Protein Information    cGMP-specific 3',5'-cyclic phosphodiesterase
 Protein Family    cGMP-specific 3',5'-cyclic phosphodiesterase
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PDE5A elisa kit
 Products by Pathway  Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
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